A focus on Ca2+ supply in microbial induced carbonate precipitation and its effect on recycled aggregate

Ouyang, Jinzhi; Liu, Kaiwei; Sun, Dongbai; Xu, Wanyu; Wang, Aiguo; Ma, Rui

doi:10.1016/j.jobe.2022.104334

Cited by 15 publications

(7 citation statements)

References 40 publications

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“…Recently, some researchers have tried to apply MICP to improve RA properties. Several scientists (Lu et al, 2022;Ouyang et al, 2022) investigated RA modification by applying MICP, and their results confirmed the effectiveness and feasibility of microbial modification. Wang et al (2017) applied MICP and found that water absorption of RAC for 6-8 mm aggregate range was improved by 14.5%, while a 13.9% increase was observed for the 12-16 mm aggregate range.…”

Section: Introductionmentioning

confidence: 80%

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Bio-deposition approaches for sustainable execution of recycled aggregates in concretes

Liu¹,

Li²,

Adeel³

et al. 2023

Front. Mater.

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Our study offers a quantitative framework for microbial-induced calcium carbonate precipitation (MICP) to uplift the properties of recycled aggregate concrete (RAC). In this regard, a marine alkalophilic bacterium Bacillus sp. B6 was employed, and its growth and mineralization efficiency under seawater conditions was investigated. Optimization of MICP was achieved with different nutrient sources and bacterial introduction methods (dip and spray). The efficiency of treated recycled aggregates (RA) was determined by using scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) in combination with basic technologies of density improvement and water absorption. The results show that the optimal nutrient sources of carbon and nitrogen are glucose (7 g L−1) and tryptone (5 gL−1), respectively. Spray treated recycled aggregate (RA) is reported to be efficient with a reduction in water absorption (∼19%), and improvement in bulk (∼2.4%) and apparent density (∼1.7%). The effectiveness of the MICP treatment process is confirmed with SEM and EDX observations, indicating the filling of microcracks with calcium carbonates. As such, the mechanical properties of RAC with spray treated RA significantly increase by up to ∼24% in compressive strength as compared to the control treatment. Current findings will help revitalize the construction industry by utilizing the MICP-treated RA and minimizing the load on natural aggregates.

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Section: Introductionmentioning

confidence: 80%

“…Recent research found that RAC can be strengthened costeffectively and environmentally friendly manner without the removal of AM (Wang et al, 2017;Zeng et al, 2019;Ouyang et al, 2022). Strengthening AM fills the micro-cracks and pores of RAC, which leads to improvements in mechanical properties and less water absorption (Kaur et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Bio-deposition approaches for sustainable execution of recycled aggregates in concretes

Liu¹,

Li²,

Adeel³

et al. 2023

Front. Mater.

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“…Therefore, measurements are taken at this specific wavelength to quantify the density or cell concentration of cyanobacteria [47]. Excessive levels of Ca 2+ concentration can impede bacterial activity [48]. In this study, the cell concentration of Synechocystis sp.…”

Section: Xps Analyses Of Biominerals Induced By Synechocystis Sp Pcc ...mentioning

confidence: 99%

Effect of Magnesium and Ferric Ions on the Biomineralization of Calcium Carbonate Induced by Synechocystis sp. PCC 6803

Zhao,

Han,

Liang

et al. 2023

Minerals

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The discovery of cyanobacteria fossils in microbialite prompts the investigation of carbonate biomineralization using cyanobacteria. However, the impact of coexisting magnesium and iron in microbialite on carbonate biomineralization has been overlooked. Here, Synechocystis sp. PCC 6803 was used to induce calcium carbonate in the presence of coexisting magnesium and ferric ions. The findings demonstrate that cell concentration, pH, carbonic anhydrase activity, and carbonate and bicarbonate concentrations decreased with increasing concentrations of magnesium and calcium ions. Ferric ions yielded a contrasting effect. The levels of deoxyribonucleic acid, protein, polysaccharides, and humic substances in extracellular polymeric substances increased in the presence of separated or coexisting calcium, magnesium, and ferric ions. Magnesium ions inhibited calcium ion precipitation, whereas ferric ions exhibited the opposite effect. Protein secondary structures became more abundant and O-C=O and N-C=O contents increased with increasing ion concentrations by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses. Scanning electron microscopy revealed that ferric ions lead to rougher surfaces and incomplete rhombohedral structures of calcite, whereas magnesium ions promoted greater diversity in morphology. Magnesium ions enhanced the incorporation of ferric ions. This work aims to further understand the effect of magnesium and ferric ions on calcium carbonate biomineralization induced by cyanobacteria.

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“…Optimal calcification protocols often involve a calcifying solution with a concentration of calcium chloride at or exceeding 0.25 M (Hirsch et al, 2023;Ouyang et al, 2022). Very few environments on Earth experience calcium salt concentrations in this range, and in these kinds of extreme environments, S. pasteurii is not an active community member, and thus is likely to experience important levels of stress when it is involved in anthropogenic microbially induced calcite precipitation (MICP) processes (Marion et al, 2003).…”

Section: Bacteria Inducing Biocalcificationmentioning

confidence: 99%

Paper waste grown as a biocalcified foam: perspectives from a bacterial and design viewpoint

Mosse,

Rennie,

Poudoulec

et al. 2024

Res. dir. Biotechnol. des.

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Biocalcification is a naturally occurring mineralisation phenomenon resulting from the urease produced by microorganisms inhabiting soil environments. This process, often referred to as microbially induced calcite precipitation (MICP), is primarily exploited in an engineering context for soil stabilization and the repair of concrete structures. MICP represents an emerging area of research in architecture and design. In this paper, we discuss the appropriation of MICP on Papier Plume, a foam made of paper waste used in the context of ImpressioVivo: a design-led research project exploring the conception and fabrication of 3D-printed and bacterially-induced bio-sourced materials for a circular design framework. In the light of a previous study based on two strategies of calcification: (1) direct inoculation (2) spraying, we - a team of two designers and a microbiologist- discuss the relevance of an immersion strategy applied to the dry paper foam substrate. By doing so, we reflect on the relevance of MICP as a material design process underpinned by sustainable and circularity concerns, from a design perspective, but also into an attempt to embrace the perspective of the bacteria supporting these experiments; namely Sporosarcina pasteurii.

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A focus on Ca2+ supply in microbial induced carbonate precipitation and its effect on recycled aggregate

Cited by 15 publications

References 40 publications

Bio-deposition approaches for sustainable execution of recycled aggregates in concretes

Bio-deposition approaches for sustainable execution of recycled aggregates in concretes

Effect of Magnesium and Ferric Ions on the Biomineralization of Calcium Carbonate Induced by Synechocystis sp. PCC 6803

Paper waste grown as a biocalcified foam: perspectives from a bacterial and design viewpoint

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