A Fluorometric Assay for the Determination of 1-Deoxy--xylulose 5-Phosphate Synthase Activity

Querol, Jordi; Besumbes, Óscar; Lois, L. María; Boronat, Albert; Imperial, Santıago

doi:10.1006/abio.2001.5234

Cited by 28 publications

(16 citation statements)

References 21 publications

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“…We found some ambiguities in early results because of potential effects of the tested metabolites on DXR. A fluorometric assay for DXS was developed using a fluorescent derivative of the product DXP (49). This assay suffered from a lack of selectivity.…”

Section: Discussionmentioning

confidence: 99%

Feedback Inhibition of Deoxy-d-xylulose-5-phosphate Synthase Regulates the Methylerythritol 4-Phosphate Pathway

Banerjee

et al. 2013

Journal of Biological Chemistry

154

186

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Background:The methylerythritol phosphate (MEP) pathway is required for the biosynthesis of plastid-derived isoprenoids from plants. Results: Deoxyxylulose-5-phosphate synthase (DXS) was cloned from Populus trichocarpa, and metabolic regulation was tested. Conclusion: Both isopentenyl diphosphate and dimethylallyl diphosphate inhibit DXS by competing with thiamine pyrophosphate. Significance: Prediction of isoprene emission from trees and bioengineering of MEP pathway will be aided by these results.

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Section: Discussionmentioning

confidence: 99%

Feedback Inhibition of Deoxy-d-xylulose-5-phosphate Synthase Regulates the Methylerythritol 4-Phosphate Pathway

Banerjee

et al. 2013

Journal of Biological Chemistry

154

186

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“…The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) was determined by the method of Toroser and Huber (1998). The activity of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) was determined by the fluorometric method according to Querol et al (2001).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Effects of abscisic acid on content and biosynthesis of terpenoids in Cannabis sativa at vegetative stage

Mansouri

Asrar

2012

Biologia plant.

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The influence of abscisic acid (ABA) on plastidial and cytosolic terpenoids and on two key enzymes for terpenoid biosynthesis was determined in vegetative stage of Cannabis sativa L. Low concentration of ABA (1 µM) increased 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity in treated plants in comparison to control plants. The amounts of chlorophyll a and carotenoids increased in response to ABA treatment but chlorophyll b content declined. The accumulation of α-tocopherol was stimulated only by 10 µM ABA. The ABA-treated plants showed a decline in 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) activity which was followed by a decrease in squalene and phytosterol content. ABA also decreased tetrahydrocannabinol (THC) and cannabidiol (CBD) contents. The essential oil had higher ratios of monoterpenes to sesquiterpenes as ABA-treated plants had less numbers of sesquiterpenes in comparison with control plants. Influence of ABA on the amounts of sesquiterpenes was different, some of them showed decrease of content and others increase of content.

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“…DXS activity was performed according to Querol et al (2001) and PAL activity was measured according to Zucker (1969), with some modifications. The reaction mixture contained 0.1 mM borate buffer (pH 8.8) and 0.05 mM L-phenylalanine.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Increasing anthraquinone production by overexpression of 1-deoxy-d-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia

et al. 2010

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A Morinda citrifolia cell line was obtained by overexpresion of 1-deoxy-D: -xylulose 5-phosphate synthase (DXS) from Catharanthus roseus, a key enzyme of the metabolic pathway of anthraquinones (AQs). This cell line increased AQs production by about 24% compared to the control cell line. This transgenic cell line which carries dxs cDNA isolated from Catharanthus roseus, was achieved by direct transformation of cell suspension cultures of M. citrifolia using a hypervirulent Agrobacterium tumefaciens strain. The effects of the overexpression of the dxs gene also resulted in increased levels of dxs mRNA transcripts and DXS activity compared to the control cell line. In addition, total phenolics and phenylalanine ammonia-lyase activity were evaluated and were significantly higher in the transgenic line than in controls.

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A Fluorometric Assay for the Determination of 1-Deoxy--xylulose 5-Phosphate Synthase Activity

Cited by 28 publications

References 21 publications

Feedback Inhibition of Deoxy-d-xylulose-5-phosphate Synthase Regulates the Methylerythritol 4-Phosphate Pathway

Feedback Inhibition of Deoxy-d-xylulose-5-phosphate Synthase Regulates the Methylerythritol 4-Phosphate Pathway

Effects of abscisic acid on content and biosynthesis of terpenoids in Cannabis sativa at vegetative stage

Increasing anthraquinone production by overexpression of 1-deoxy-d-xylulose-5-phosphate synthase in transgenic cell suspension cultures of Morinda citrifolia

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