A fluorometric and optical signal dual-readout detection of alkaline phosphatase activity in living cells based on ATP-mediated porphyrin MOFs

Song, Yanxia; Lu, Siyu; Sun, Shasha; Guo, Wenting; Su, Junxia; Meng, Genping; Hai, Jun; Wang, Baodui

doi:10.1016/j.snb.2021.130017

Cited by 12 publications

(3 citation statements)

References 56 publications

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“…18 Thus, a real-time, noninvasive, and highly accurate evaluation of ALP activity is essential for clinical diagnosis. So far, several conventional techniques for ALP determination have been exploited, such as electrochemistry, 19 fluorescence, 20 surface enhancement Raman scattering (SERS), 21 and colorimetric protocols. 22 Among these strategies, fluorescence analysis methods are particularly prominent owing to their inherent merits of excellent sensitivity, easy operability, and cost effectiveness.…”

Section: ■ Introductionmentioning

confidence: 99%

Lysozyme-Functionalized 5-Methyl-2-thiouracil Gold/Silver Nanoclusters for Luminescence Assay of Alkaline Phosphatase

Wang

Zhou

Cheng

2021

ACS Appl. Nano Mater.

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Herein, lysozyme-functionalized 5-methyl-2-thiouracil gold/silver nanoclusters (5-MTU/Lys Au/Ag NCs) with an orange-red fluorescence peak at 600 nm (quantum yield = 33.18%) were obtained through a simple blending route. Owing to the synergistic effect between Au and Ag and the formation of rigid host–guest assemblies between 5-MTU and the guanidine group of lysozymes, the chemical properties of 5-MTU/Lys Au/Ag NCs were significantly improved. Taking advantage of the exceptional optical properties and satisfactory stability of 5-MTU/Lys Au/Ag NCs, a fresh and sensitive fluorescent probe for monitoring alkaline phosphatase (ALP) was strategically constructed by the integration of 5-MTU/Lys Au/Ag NCs and the nanozyme with a graphene structure (Fe-G nanozyme) for the first time. Briefly, the fluorescence of 5-MTU/Lys Au/Ag NCs was initially quenched by indigo carmine (IC) through the inner filter effect (IFE) mechanism, but it was restored upon the introduction of H2O2 and Fe-G nanozyme by generating reactive oxygen species (ROS) (1O2/O2 •–) and promoting the oxidative degradation of IC, whereas the fluorescence was quenched again in the presence of ALP via hydrolyzing l-ascorbic acid-2-phosphate (AAP). By monitoring the changes in signal intensity, the proposed fluorescent sensing platform presented a highly selective and excellent performance toward ALP, exhibiting a good linear relationship from 0.5 to 10 U L–1 and providing a low detection limit of 0.193 U L–1. Furthermore, the proposed biosensor showed satisfactory results for quantifying ALP in human serum.

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Section: ■ Introductionmentioning

confidence: 99%

Lysozyme-Functionalized 5-Methyl-2-thiouracil Gold/Silver Nanoclusters for Luminescence Assay of Alkaline Phosphatase

Wang

Zhou

Cheng

2021

ACS Appl. Nano Mater.

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“…Numerous analytics have been developed for the ALP and IL-1β detection including surface-enhanced Raman spectroscopy, − fluorometric, − colorimetric, − electrochemical, ,− and electrical sensors. − Among them, the colorimetric sensors have proven to be most powerful and effective analytics for early diagnosis with simple and fast quantitative analytical performance based on a reduced instrumentation complexity, enabling them interfaced with a point-of-care (POC) assay. , The colorimetric signaling mechanism of the ALP assay is based on the chromogenic signal difference between the phosphate and the de-phosphated species . Ascorbic acid phosphate (AAP) has been utilized as a specific substrate to initiate the chromogenic signal in the ALP assay. − However, this is fundamental limitation for accurate diagnosis because AAP is being used as therapeutic elements to enhance the osseointegration of dental implants and improve post-surgical periodontal healing, forming baseline interfering signal of the periodontal analytes and prohibiting the accurate ALP quantification. − Therefore, it is critically required to design a signaling pathway that can produce diverse signal readout based on colorimetric responses for achieving multivariate analysis, high-throughput diagnosis, and versatility in dental applications.…”

Section: Introductionmentioning

confidence: 99%

Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

et al. 2023

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Alkaline phosphatase (ALP) and interleukin-1beta (IL-1β) are crucial salivary biomarkers for the diagnosis of periodontal disease that harms the periodontal tissue along with tooth loss. However, there has been no way of sensitive and portable detection of both biomarkers in saliva with multivariate signal readout. In this work, we design the multicolorimetric ALP and IL-1β sensing platform based on geometrical transformation of silver nanoplate transducer. By utilizing enzymatic activity of ALP that dephosphorylates p-aminophenol phosphate (p-APP) to p-aminophenol (p-AP), localized surface plasmon resonance properties of silver nanoplate vary with ALP and show a distinct color change from blue to yellow based on a controlled seed transformation from triangular to hexagonal, rounded pentagonal, and spherical shape. The multicolor sensor shows an ALP detection range of 0–25 U/L with a limit of detection (LOD) of 0.0011 U/L, which is the lowest range of LOD demonstrated to date for state-of-the-art ALP sensor. Furthermore, we integrate the sensor with the conventional ELISA to detect IL-1β for multicolor signaling and it exhibits a linear detection range of 0–250 pg/mL and an LOD of 0.066 pg/mL, which is 2 orders of magnitude lower than the monochromic conventional ELISA (LOD of 3.8 pg/mL). The ALP multicolor sensor shows high selectivity with a recovery of 100.9% in real human saliva proving its reliability and suitability for the readily accessible periodontal diagnosis with multivariate signal readout.

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“…Traditional detection methods for sensing carbaryl mainly include gas chromatography, liquid chromatography-mass spectrometry, electroanalytical measurement, and colorimetry. − Some disadvantages such as complex sample pretreatment, cumbersome instrument operation, and high cost limit the practical application of the abovementioned methods. , Therefore, it is urgent to find a convenient and cheap method for sensing carbaryl. According to some literature studies previously reported, − the researchers generally consider that fluorescence sensing is one of the ideal methods for the detection of carbaryl due to its simple operation, low cost, and visual characteristics.…”

Section: Introductionmentioning

confidence: 99%

Porous MB@Cd-MOF Obtained by Post-Modification: Self-Calibrated Fluorescent Turn-on Sensor for Highly Sensitive Detection of Carbaryl

Zhang

Gao

2022

Crystal Growth & Design

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Abuse of pesticides has caused great threat to the environment, so it is urgent to find a fast and sensitive method to detect pesticide residues in agricultural products and water. Herein, a 3D Cd-MOF was designed and synthesized by the reaction of 3,5-di(2′,5′-dicarboxylphenyl)pyridine (H 4 DDPP) and Cd(NO 3 ) 2 under solvothermal conditions. Meanwhile, a stable MB@Cd-MOF composite with dual-emitting characteristic was constructed by the in situ encapsulation of methylene blue (MB) into the channel of Cd-MOF. Compared with Cd-MOF, MB@Cd-MOF exhibits sensitive sensing for carbaryl with the detection limit of 6.7 ng•mL −1 and high accuracy, which are attributed to the fluorescence enhancement effect and dual-emitting characteristics

show abstract

A fluorometric and optical signal dual-readout detection of alkaline phosphatase activity in living cells based on ATP-mediated porphyrin MOFs

Cited by 12 publications

References 56 publications

Lysozyme-Functionalized 5-Methyl-2-thiouracil Gold/Silver Nanoclusters for Luminescence Assay of Alkaline Phosphatase

Lysozyme-Functionalized 5-Methyl-2-thiouracil Gold/Silver Nanoclusters for Luminescence Assay of Alkaline Phosphatase

Detection of Periodontal Disease Marker with Geometrical Transformation of Ag Nanoplates

Porous MB@Cd-MOF Obtained by Post-Modification: Self-Calibrated Fluorescent Turn-on Sensor for Highly Sensitive Detection of Carbaryl

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