A fluorescent aptasensor for sensitive detection of isocarbophos based on AT-rich three-way junctions DNA templated copper nanoparticles and Fe3O4@GO

Fan, Kaimei; Yang, Ruifu; Zhao, Yunwei; Zang, Chuanyao; Miao, Xiangshui; Qu, Baihua; Lu, Lihua

doi:10.1016/j.snb.2020.128515

Cited by 44 publications

(13 citation statements)

References 47 publications

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“…Polydiacetylene (PDA) liposomes were immobilized with an aptamer-tagged fluorophore (Cy3–Apt) for assaying mucin 1 (MUC1), as indicated in the proposed scheme shown in Figure 6 H, with a LOD of 0.8 nM [ 133 ], based on the spectral overlap of Cy3’s fluorescence emission and the absorbance of PDA–Apt liposomes [ 133 ]. Fan et al synthesized Trich three-way junction (AT-TWJ) DNA-stabilized CuNPs as a fluorescent transducer and magnetic Fe 3 O 4 @GO as a single-stranded DNA (ssDNA) adsorbent for the detection of isocarbophos [ 134 ] ( Figure 6 I). The dsDNA is hybridized by the isocarbophos aptamer, while the complementary DNA plays a role as a recognition unit.…”

Section: Fluorescence Aptasensormentioning

confidence: 99%

“…( H ) Schematic showing the fabrication of PDA–Apt liposomes and fluorescence imaging of plasma membrane glycoprotein MUC1, reproduced from [ 133 ]. ( I ) Schematic description of isocarbophos assay via fluorometric aptsensor based on AT-rich three-way junction DNA template copper nanoparticles and Fe 3 O 4 @GO, reproduced from [ 134 ].…”

Section: Fluorescence Aptasensormentioning

confidence: 99%

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Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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Section: Fluorescence Aptasensormentioning

confidence: 99%

Section: Fluorescence Aptasensormentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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“…As molecular recognition elements, aptamers are artificially synthesized single-stranded nucleotide sequences, screened through systematic evolution of ligands by exponential enrichment (SELEX) techniques, and could specifically bind the target analyte (12). Aptamers have attracted significant attention because of their advantages of easy synthesis, low cost, and high affinity (13). Unlike antibodies, aptamers are relatively stable under extreme temperature conditions for a short time (14).…”

Section: Introductionmentioning

confidence: 99%

A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

Zhai

Liu

et al. 2022

Front. Nutr.

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Carbendazim (CBZ), a systemic, broad-spectrum benzimidazole fungicide, is widely used to control fungal diseases in agricultural products. Its residues might pose risks to human health and the environment. Therefore, it is warranted to establish a rapid and reliable method for its residual quantification. Herein, we proposed a competitive assay that combined aptamer (DNA) specific recognition and bimetallic nanozyme gold@platinum (Au@Pt) catalysis to trace the CBZ residue. The DNA was labeled onto bimetallic nanozyme Au@Pt surface to produce Au@Pt probes (Au@Pt-DNA). The magnetic Fe3O4 was functionalized with a complementary strand of DNA (C-DNA) to form Fe3O4 probes (Fe3O4-C-DNA). Subsequently, the CBZ and the Fe3O4 probes competitively react with Au@Pt probes to form two Au@Pt-DNA biosensors (Au@Pt-ssDNA-CBZ and Au@Pt-dsDNA-Fe3O4). The Au@Pt-ssDNA-CBZ biosensor was designed for qualitative analysis through a naked-eye visualization strategy in the presence of CBZ. Meanwhile, Au@Pt-dsDNA-Fe3O4 biosensor was developed to quantitatively analyze CBZ using a multifunctional microplate reader. A competitive assay based on the dual-mode Au@Pt-DNA biosensors was established for onsite sensitive determination of CBZ. The limit of detection (LOD) and recoveries of the developed assay were 0.038 ng/mg and 71.88-110.11%, with relative standard deviations (RSDs) ranging between 3.15 and 10.91%. The assay demonstrated a good correlation with data acquired from liquid chromatography coupled with mass spectrometry/mass spectrometry analysis. In summary, the proposed competitive assay based on dual-mode Au@Pt-DNA biosensors might have a great potential for onsite sensitive detection of pesticides in agro-products.

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“…T junctions (also called three-way junctions) that formed of three strands of DNA rely on the Watson–Crick complementary base pairing principle and are a type of stable architecture with demonstrated versatility in the biosensing field. , Cheng et al reported a kind of defective T junction that contained an artificial vacuole at the junction site and was more conformationally stable than the traditional T junction . To overcome the shortcomings of the normal SDA strategy, herein, through the ingenious design of a pair of dual-function probes, a new SDA method in which the defective T junction structure is first used to support SDA (dT-SDA) is proposed.…”

Section: Introductionmentioning

confidence: 99%

Study on Defective T Junction-Mediated Strand Displacement Amplification and Its Application in Microchip Electrophoretic Detection of Longer Bacterial 16S rDNA

et al. 2021

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Current strand displacement amplification (SDA)-based nucleic acid sensing methods generally rely on a ssDNA template that involves complementary bases to the endonuclease recognition sequence, which has the limitation of detecting only short nucleic acids. Herein, a new SDA method in which the defective T junction structure is first used to support SDA (dT-SDA) was proposed and applied in longer DNA detection. In dT-SDA, an auxiliary probe and a primer were designed to specifically identify the target gene, following the formation of a stable defective T junction structure through proximity hybridization, and the formation of defective T junctions could further trigger cascade SDA cycling to produce numerous ssDNA products. The quantity of these ssDNA products was detected through microchip electrophoresis (MCE) and could be transformed to the concentration of the target gene. Moreover, the applicability of this developed strategy in detecting long genomic DNA was verified by detecting bacterial 16S rDNA. This proposed dT-SDA strategy consumes less time and has satisfactory sensitivity, which has great potential for effective bacterial screening and infection diagnosis.

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A fluorescent aptasensor for sensitive detection of isocarbophos based on AT-rich three-way junctions DNA templated copper nanoparticles and Fe3O4@GO

Cited by 44 publications

References 47 publications

Recent Advances in Aptamer Sensors

Recent Advances in Aptamer Sensors

A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

Study on Defective T Junction-Mediated Strand Displacement Amplification and Its Application in Microchip Electrophoretic Detection of Longer Bacterial 16S rDNA

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