A Fluorescence-Based Assay for Measuring the Redox Potential of 5-Lipoxygenase Inhibitors

Lee, Sang-Chul; Park, Young-Sam; Kim, Junghwan; Han, Sung‐Jun

doi:10.1371/journal.pone.0087708

Cited by 2 publications

(1 citation statement)

References 22 publications

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“…Traditionally, virtually all studies concerned with the synthesis of redox modulators, pro- and antioxidants alike, have also reported some information related to the redox activity and catalytic behaviour of such compounds in vitro, for instance in radical scavenging assays, specific glutathione peroxidase assays, dye-based redox assays or electrochemical studies [ 106 , 107 , 108 , 109 , 110 ]. Nowadays, an increasing number of publications also provides evidence in cell culture, ranging from bacteria, fungi and yeasts to mammalian cells.…”

Section: Targets For Redox Catalysts: a Selected Manymentioning

confidence: 99%

Small Molecule Catalysts with Therapeutic Potential

et al. 2018

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Catalysts are employed in many areas of research and development where they combine high efficiency with often astonishing selectivity for their respective substrates. In biology, biocatalysts are omnipresent. Enzymes facilitate highly controlled, sophisticated cellular processes, such as metabolic conversions, sensing and signalling, and are prominent targets in drug development. In contrast, the therapeutic use of catalysts per se is still rather limited. Recent research has shown that small molecule catalytic agents able to modulate the redox state of the target cell bear considerable promise, particularly in the context of inflammatory and infectious diseases, stroke, ageing and even cancer. Rather than being “active” on their own in a more traditional sense, such agents develop their activity by initiating, promoting, enhancing or redirecting reactions between biomolecules already present in the cell, and their activity therefore depends critically on the predisposition of the target cell itself. Redox catalysts, for instance, preferably target cells with a distinct sensitivity towards changes in an already disturbed redox balance and/or increased levels of reactive oxygen species. Indeed, certain transition metal, chalcogen and quinone agents may activate an antioxidant response in normal cells whilst at the same time triggering apoptosis in cancer cells with a different pre-existing “biochemical redox signature” and closer to the internal redox threshold. In pharmacy, catalysts therefore stand out as promising lead structures, as sensor/effector agents which are highly effective, fairly selective, active in catalytic, i.e., often nanomolar concentrations and also very flexible in their structural design.

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Section: Targets For Redox Catalysts: a Selected Manymentioning

confidence: 99%

Small Molecule Catalysts with Therapeutic Potential

et al. 2018

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Signal Improvement Strategies for Fluorescence Detection of Biomacromolecules

2016

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For analysis of biomacromolecules, a sensitive, specified and reliable method is indispensable. Fluorescent dyes or fluorophores have been widely used as mediums to obtain readout signals in various assays or bioimaging because of their versatilities such as biocompatibility. Those fluorescent dyes based techniques manipulate many molecular interactions for analysis of biomacromolecules including antibody-protein interaction, base complementation, glycan-lectin interaction, etc. The strategies to manipulate those molecular interactions are various and always updating due to the development of biotechnological tools and instruments. In this minireview, we summarize the state of the art of signal improvement techniques for fluorescence detection of biomacromolecules especially proteins and nucleic acids. We focus on the principle and mechanism of those techniques for fluorescence detection of biomacromolecules. We also discuss the future trend of the techniques for fluorescence detection of biomacromolecules.

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A Fluorescence-Based Assay for Measuring the Redox Potential of 5-Lipoxygenase Inhibitors

Cited by 2 publications

References 22 publications

Small Molecule Catalysts with Therapeutic Potential

Small Molecule Catalysts with Therapeutic Potential

Signal Improvement Strategies for Fluorescence Detection of Biomacromolecules

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