A Fluorescence Approach to Assess the Production of Soluble Microbial Products from Aerobic Granular Sludge Under the Stress of 2,4-Dichlorophenol

Abstract

In this study, a fluorescence approach was used to evaluate the production of soluble microbial products (SMP) in aerobic granular sludge system under the stress of 2,4-dichlorophenol (2,4-DCP). A combined use of three-dimension excitation emission matrix fluorescence spectroscopy (3D-EEM), Parallel factor analysis (PARAFAC), synchronous fluorescence and two-dimensional correlation spectroscopy (2D-COS) were explored to respect the SMP formation in the exposure of different doses of 2,4-DCP. Data implied that the presence of 2,4-DCP had an obvious inhibition on biological nitrogen removal. According to EEM-PARAFAC, two fluorescent components were derived and represented to the presence of fulvic-like substances and humic-like substances in Component 1 and protein-like substances in Component 2. It was found from synchronous fluorescence that protein-like peak presented slightly higher intensity than that of fulvic-like peak. 2D-COS further revealed that fluorescence change took place sequentially in the following order: protein-like fraction > fulvic-like fraction. The obtained results could provide a potential application of fluorescence spectra in the released SMP assessment in the exposure of toxic compound during wastewater treatment.

Spectroscopic characteristics of dissolved organic matter from aquaculture wastewater and its interaction mechanism to chlorinated phenol compound

“…For both samples, a strong and broad band approximately around 3421-3424 cm −1 could be attributed to the overlap of OH stretching of phenol, carbohydrate and carboxylic acid compounds, as similarly reported by Abdulla et al [40]. The band at 1625-1628 cm −1 represented the C=O stretching vibration of Amide I from protein [27]. A small band near 1396 cm −1 may be attributed to symmetrical stretching of COO groups associated with amino acids.…”

“…In present study, aromatic protein-like substances showed the maximum fluorescence intensity than those of other substances in raw DOM, which may be attributed to the microbial activity during aquaculture process. Moreover, it has been reported that protein-like substances in the effluent of treated wastewater were more sensitive to the presence of toxic compound in aerobic granular sludge system [27]. Compared with DOM from aquaculture wastewater, the majority of DOM consisted of humic-like substances during the landfill process of MSW, which were much more difficult for the microorganisms to utilize than that of protein-like substances [28].…”

Performance, microbial community and fluorescent characteristic of microbial products in a solid-phase denitrification biofilm reactor for WWTP effluent treatment

“…Component 2 also had two obvious peaks that occurring around Ex/Em of 350/440 and 250/440 nm, which were identified as humic-like and fulvic acid-like substances. Similar two kinds of components from PARAFAC have been also identified from SMP released from aerobic granular sludge under the stress of 2, 4-Dichlorophenol (Wei et al, 2016). Fig.…”

Nitrogen removal via nitrite in a partial nitrification sequencing batch biofilm reactor treating high strength ammonia wastewater and its greenhouse gas emission

“…S3 displays the synchronous fluorescence spectra of two types of EPS from seed sludge and PN-biofilm. The whole wavelength in synchronous fluorescence spectra could be divided into three ranges of 250-300, 300-380 and 380-550 nm, assigning to the presence of protein-like, fulvic-like, and humic-like fluorescence fractions,respectively(Wei et al, 2016). It was obviously observed that the both LB-EPS and TB-EPS in PN-biofilm had higher fluorescent intensities than those of seed sludge.Moreover, a much higher fluorescent intensity was observed at 285 nm than other two kinds of fractions, suggesting that the protein-like fraction was the predominant component in EPS, as similarly with the result of chemical and EEM analysis.…”