A fluoran-based viscosity probe with high-performance for lysosome-targeted fluorescence imaging

Han, Defang; Yi, Jundan; Chang, L.K.; Liang, Lijuan; Huang, Kun; Jing, Lin‐Hai; Qin, Da‐Bin

doi:10.1016/j.saa.2020.118405

Cited by 31 publications

(7 citation statements)

References 55 publications

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“…As shown in Figure S7 , the fluorescent signal of Chi_FITC increased with the increase in pH, but in the presence of 10C MMT and 0.1C MMT, the pH responsiveness of the dye was completely lost, even at low concentrations, which can be ascribed to the stabilization of the electron distribution of FITC upon interaction with MMT ( Figure S7 ). Such behavior of FITC interacting with MMT will be useful in designing optical hybrid materials for applications where the changes in fluorescence intensity upon pH variation are undesirable, such as in imaging and sensing [ 58 , 59 ].…”

Section: Resultsmentioning

confidence: 99%

Changes in Optical Properties upon Dye–Clay Interaction: Experimental Evaluation and Applications

2021

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The development of hybrid materials with unique optical properties has been a challenge for the creation of high-performance composites. The improved photophysical and photochemical properties observed when fluorophores interact with clay minerals, as well as the accessibility and easy handling of such natural materials, make these nanocomposites attractive for designing novel optical hybrid materials. Here, we present a method of promoting this interaction by conjugating dyes with chitosan. The fluorescent properties of conjugated dye–montmorillonite (MMT) hybrids were similar to those of free dye–MMT hybrids. Moreover, we analyzed the relationship between the changes in optical properties of the dye interacting with clay and its structure and defined the physical and chemical mechanisms that take place upon dye–MMT interactions leading to the optical changes. Conjugation to chitosan additionally ensures stable adsorption on clay nanoplatelets due to the strong electrostatic interaction between chitosan and clay. This work thus provides a method to facilitate the design of solid-state hybrid nanomaterials relevant for potential applications in bioimaging, sensing and optical purposes.

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Section: Resultsmentioning

confidence: 99%

Changes in Optical Properties upon Dye–Clay Interaction: Experimental Evaluation and Applications

2021

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“…As displayed in Figure 18, the Qin group developed a spironolactone fluoran‐based fluorescent probe 38 for imaging of lysosomal viscosity [65] . Probe 38 had low cytotoxicity as well as high sensitivity and selectivity to viscosity under different pH values.…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

108

115

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Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

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“…The changes of pH and viscosity in cells will affect the reaction rate and material and energy transfer rates of cells, which have important biological value for the analysis of various physiological activities of cells (Han et al, 2020;Kim et al, 2020;Ma et al, 2020;Zhi et al, 2020). A novel AIE-active probe 3 based on a styrylquinoline derivative with excellent two-photon performance was designed and synthesized ( Figure 2B) (Dou et al, 2019).…”

Section: Bioimaging Applications Fluorescent Detectionmentioning

confidence: 99%

Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications

Lü

Liu

et al. 2020

Front. Chem.

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Fluorescence imaging has been widely used as a powerful tool for in situ and real-time visualization of important analytes and biological events in live samples with remarkably high selectivity, sensitivity, and spatial resolution. Compared with one-photon fluorescence imaging, two-photon fluorescence imaging exhibits predominant advantages of minimal photodamage to samples, deep tissue penetration, and outstanding resolution. Recently, the aggregation-induced emission (AIE) materials have become a preferred choice in two-photon fluorescence biological imaging because of its unique bright fluorescence in solid and aggregate states and strong resistance to photobleaching. In this review, we will exclusively summarize the applications of AIE-active materials in two-photon fluorescence imaging with some representative examples from four aspects: fluorescence detection, in vitro cell imaging, ex vivo tissue imaging, and in vivo vascular imaging. In addition, the current challenges and future development directions of AIE-active materials for two-photon bioimaging are briefly discussed.

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A fluoran-based viscosity probe with high-performance for lysosome-targeted fluorescence imaging

Cited by 31 publications

References 55 publications

Changes in Optical Properties upon Dye–Clay Interaction: Experimental Evaluation and Applications

Changes in Optical Properties upon Dye–Clay Interaction: Experimental Evaluation and Applications

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Fluorescent AIE-Active Materials for Two-Photon Bioimaging Applications

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