A flow injection biamperometric method for determination of total antioxidant capacity of alcoholic beverages using bienzymatically produced ABTS+

Methods for Total Antioxidant Activity Determination: A Review

“…The linearity of IDE detector was tested in the range 20µM-2000µM and a good sensitivity of 0.165 nA/µM for Trolox solutions was obtained [100]. The interdigitated gold electrodes were used for biamperometric determination of antioxidant capacity of alcoholic beverages (wine and spirits) [100].…”

“…Normalphase HPLC uses a polar stationary phase and a non-polar, nonaqueous mobile phase, and works effectively for separating analytes readily soluble in non-polar solvents. Reversed phase HPLC has a capacity assay is ABTS +• /ABTS [99,100]. The ABTS cation radical was The antioxidant activity using a HPLC system with postcolumn on-line antioxidant detection, based on 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid radical scavenging activity.…”

Electrochemical Methods for Total Antioxidant Capacity and its Main Contributors Determination: A review

“…The confirmed high selectivity of the biamperometric method allows the quantification of redox active analytes present in biological samples (urine, blood), and, also, of the total antioxidant capacity of foodstuffs and beverages [113][114][115][116]. Nevertheless, biamperometry requires very rigorous control of experimental conditions; the method's performances with respect to selectivity relying upon the specificity of the reaction between the oxidized or reduced form of the redox couple and the analyte [113][114][115][116].…”

“…A peroxidase-containing flow system generated the cation radical, aiming at the assessment of antioxidant activity in juices, tea and wine at interdigitated microelectrodes [115], or the cation radical could be obtained by using glucose oxidase and peroxidase, for the analysis of wine and spirits with enhanced sensitivity, 0.165 nA/μM Trolox [116].…”

Biamperometric Applications to Antioxidant Content and Total Antioxidant Capacity Assessment: An Editorial

“…ABTS +. was also obtained by glucose oxidase -peroxidase, for the analysis of wine and spirits with excellent sensitivity, 0.165 nA/μM Trolox [16]. [17].…”