A flow cytometric cell-cycle assay using methyl green

Murgai, Pooja; Sharma, Prashant; Sachdeva, Man Updesh Singh; Bose, Parveen; Gupta, Mahendra; Das, Reena; Varma, Neelam

doi:10.1016/j.ab.2020.113782

Cited by 3 publications

(2 citation statements)

References 9 publications

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“…The initial dye concentrations varied from 100 to 400 mg L −1 . Simultaneously, standard curve for dyes were plotted by measuring concentration of MG at 633 nm and MO at 465 nm through UV-Vis spectrophotometer (Hitachi U-2900) ( Li et al, 2015 ; Murgai et al, 2020 ). The equilibrium quantity of dye adsorption was calculated as follows:

where Q e (mg g −1 ) is the capacity of dye adsorbed per gram hydrogel, C 0 (mg L −1 ) and C e (mg L −1 ) are the initial and equilibrium concentration of the dye, V (L) is the volume of the dye solution, and m (g) is the weight of dried hydrogel utilized in this study.…”

Section: Methodsmentioning

confidence: 99%

“…The initial dye concentrations varied from 100 to 400 mg L −1 . Simultaneously, standard curve for dyes were plotted by measuring concentration of MG at 633 nm and MO at 465 nm through UV-Vis spectrophotometer (Hitachi U-2900) (Li et al, 2015;Murgai et al, 2020). The equilibrium quantity of dye adsorption was calculated as follows:…”

Section: Adsorption Performance Of Hydrogelmentioning

confidence: 99%

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Green Synthesis of pH-Responsive, Self-Assembled, Novel Polysaccharide Composite Hydrogel and Its Application in Selective Capture of Cationic/Anionic Dyes

Srivastava

Choudhury

2021

Front. Chem.

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Dyes are one of the most hazardous chemicals causing significant environmental pollution and affecting water quality. Majority of the existing methods for dye removal and degradation involve synthetic membranes and use of hazardous chemicals, further resulting in secondary pollution. The present study reports polysaccharide based novel composite hydrogel as biodegradable matrix for pH-responsive selective adsorption of cationic/anionic dyes. This membrane showed pH-responsive adsorption of methyl green (MG) and methyl orange (MO) with similar adsorption equilibrium, i.e., 315 and 276 mg g−1, respectively. Interestingly, selective adsorption at different pH has allowed separation of dye mixtures that holds incredible industrial importance for dyes recovery. The hydrogel matrix was able to completely separate MG, a model cationic dye at neutral pH from the dye mixture whereas, it was possible to remove 60% MO, a model anionic dye at acidic pH. Furthermore, comprehensive isothermal and kinetic studies of adsorption revealed that Freundlich isotherm describing the multilayer coverage and pseudo-second-order kinetics were followed. Thermodynamic studies indicated that the adsorption process was spontaneous and endothermic. In fact, the membrane was reusable for at least ten cycles and exhibited desorption efficiency of 80 and 60% for MO and MG, respectively, which may be further recycled to make the process environmentally sustainable. Overall, this study proposes an inexpensive, simple, biologically safe, and efficient adsorbent material for dye effluent treatment.

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Section: Methodsmentioning

confidence: 99%

“…The initial dye concentrations varied from 100 to 400 mg L −1 . Simultaneously, standard curve for dyes were plotted by measuring concentration of MG at 633 nm and MO at 465 nm through UV-Vis spectrophotometer (Hitachi U-2900) (Li et al, 2015;Murgai et al, 2020). The equilibrium quantity of dye adsorption was calculated as follows:…”

Section: Adsorption Performance Of Hydrogelmentioning

confidence: 99%