A flexible immunosensor based on the electrochemically rGO with Au SAM using half-antibody for collagen type I sensing

Gwiazda, Marcin; Kaushik, Ajeet; Chlanda, Adrian; Kijeńska‐Gawrońska, Ewa; Jagiełło, Joanna; Kowiorski, Krystian; Lipińska, L.; Święszkowski, Wojciech; Bhardwaj, Sheetal K.

doi:10.1016/j.apsadv.2022.100258

Cited by 16 publications

(9 citation statements)

References 70 publications

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“…The combination of β-CD functioning as complimentary recognition sites in conjunction with MIP, which increases the template imprinting during MIP fabrication . Graphene and its derivatives have been extensively studied for electrochemical biosensing applications, in conjunction with antibodies as immunosensors , and aptamers . Graphene oxide (GO) is an attractive interface material which can be produced inexpensively by wet oxidation and exfoliation of graphite in comparison to traditional electrochemical electrodes.…”

Section: Introductionmentioning

confidence: 99%

Development of a Redox-Label-Doped Molecularly Imprinted Polymer on β-Cyclodextrin/Reduced Graphene Oxide for Electrochemical Detection of a Stress Biomarker

Goyal

Sakata

2022

ACS Omega

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Cortisol is a major stress biomarker involved in the regulation of metabolic and immune responses. Readily accessible assays with sufficient quantitative and temporal resolution can assist in prevention, early diagnosis, and management of chronic diseases. Whereas conventional assays are costly in terms of time, labor, and capital, an electrochemical approach offers the possibility of miniaturization and detection at the point-of-care. Here, we investigate the biosensor application of molecularly imprinted polypyrrole (PPy) doped with hexacyanoferrate (HCF) and coupled to reduced graphene oxide functionalized with β-cyclodextrin (β-CD). β-CD provides an inclusion site for lipophilic cortisol and was electrochemically grafted simultaneous with reduction of GO. Next, PPy was electrochemically deposited in presence of cortisol template with HCF dopant ions serving as intrinsic redox probe. Thus, the sensor response was evaluated via changes of redox peak current in cyclic voltammetry and demonstrated a broad logarithmic detection range (5 pg/mL to 5000 ng/mL, R 2 = 0.995), with a sensitivity of 8.809 μA log –1 (ng/mL) cm –2 and LOD of 19.3 pM. The sensor was shown to be specific toward cortisol in reference to salivary cortisol concentration in saliva over structural analogues. The sensor was exhibited to determine cortisol in artificial saliva at normal and elevated levels. The good performance and facile electrochemical fabrication of this antibody- and external label-free interface are promising for the development of affordable point-of-care biosensors.

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Section: Introductionmentioning

confidence: 99%

Development of a Redox-Label-Doped Molecularly Imprinted Polymer on β-Cyclodextrin/Reduced Graphene Oxide for Electrochemical Detection of a Stress Biomarker

Goyal

Sakata

2022

ACS Omega

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“…The combination of the high specificity of aptamers and their affinity for anchoring onto AuNPs, by covalent SÀ Au bond, with the simplicity of synthetic procedure of the AuNPs/GO. This characteristic represents an alternative in developing portable devices, such as in situ biomarkers and wearable sensors with electrochemical detection, as already reported in the literature [21]. The electrochemical approach also facilitates the development of integrated systems of the so-called Artificial Intelligence (AI) and Internet of Medical Things (IoMT), which is a networked bioanalytical tool with the goal of bringing doctor-patient closer together and simplifying and speeding up the exchange of data and subsequent forms of treatment.…”

Section: Introductionmentioning

confidence: 74%

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

2023

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Cortisol is a steroid hormone naturally produced by the adrenal glands. It participates in and controls several processes in the body and is considered an important physiological biomarker. Due to its very low concentrations in body fluids, its detection requires high sensitivity and specificity. Here, we present a simple electrochemical biosensor based on reduced graphene oxide (rGO) modified with gold nanoparticles (AuNPs) for the immobilization of the cortisol‐specific aptamer (Ap) (MCH‐Ap‐AurGO/GCE). Important analytical parameters for identifying the target analyte were optimized, such as conditions and amount of immobilized Ap, the influence of the concentration and nature of the supporting electrolyte, pH of the medium, and incubation time. The optimized conditions for the aptasensor were: concentration of Ap 1.0 × 10−6 mol L−1, support electrolyte Tris/HCl 50 mmol L−1, MgCl2 10 mmol L−1, and NaCl 10 mmol L−1, at pH 5.0 and incubation time of 15 min. A linear response range was obtained from 1 × 10−18 up to 1 × 10−11 mol L−1 of cortisol with a detection limit (LOD) of 1.0 × 10−18 mol L−1. A curve adjusted for operational purposes in the saliva sample was fitted for the concentration range between 0.5 × 10−14 and 1 × 10−11 mol L−1, with a linear regression equation ΔRtc/Rtc1 = 2.70 + 0.17 × log([Cortisol]). The aptasensor demonstrated a great potential for detecting cortisol in a simple, fast, and highly sensitive way, opening its path for application in real samples, which present levels below the concentration in which cortisol is commonly found in body fluids.

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“…After antibody conjugation, a new band of Amide III (C–N stretching) appears at 1220–1230 cm −1 (Fig. 4 b) 41 . Other bands related to the MoS 2 structure are also present in the antibody conjugated L-Cysteine/MoS 2 sample.…”

Section: Resultsmentioning

confidence: 98%

L-cysteine/MoS2 modified robust surface plasmon resonance optical fiber sensor for sensing of Ferritin and IgG

Thawany

Khanna

Tiwari

et al. 2023

Sci Rep

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L-cysteine conjugated molybdenum disulphide (MoS2) nanosheets have been covalently attached to a gold coated surface plasmon resonance (SPR) optical fiber to prepare a robust and stable sensor. Owing to the multifunctionality of the deposited nanosheet conjugate, the antibodies are also covalently conjugated in the subsequent step to realize the design of a SPR optical fiber biosensor for the two important bioanalytes namely, Ferritin and Immunoglobin G (IgG). The different stages of the biosensor preparation have been characterized and verified with microscopic and spectroscopic techniques. A uniform and stable deposition of the L-cysteine/MoS2 nanosheets has allowed the biosensor to be reused for multiple times. Unlike the peeling-off of the MoS2 coatings from the gold layer reported previously in the case of physically adsorbed nanomaterial, the herein adopted strategy addresses this critical concern. It has also been possible to use the single SPR fiber for both Ferritin and IgG bioassay experiments by regenerating the sensor and immobilizing two different antibodies in separate steps. For ferritin, the biosensor has delivered a linear sensor response (SPR wavelength shifts) in the concentration range of 50–400 ng/mL, while IgG has been successfully sensed from 50 to 250 µg/mL. The limit of detection for Ferritin and IgG analysis have been estimated to be 12 ng/mL and 7.2 µg/mL, respectively. The biosensors have also been verified for their specificity for the targeted molecule only. A uniform and stable deposition of the nanomaterial conjugate, reproducibility, regeneration capacity, a good sensitivity, and the specificity can be highlighted as some of key features of the L-cysteine/MoS2 optical fiber biosensor. The system can be advocated as a useful biosensor setup for the sensitive biosensing of Ferritin and IgG.

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A flexible immunosensor based on the electrochemically rGO with Au SAM using half-antibody for collagen type I sensing

Cited by 16 publications

References 70 publications

Development of a Redox-Label-Doped Molecularly Imprinted Polymer on β-Cyclodextrin/Reduced Graphene Oxide for Electrochemical Detection of a Stress Biomarker

Development of a Redox-Label-Doped Molecularly Imprinted Polymer on β-Cyclodextrin/Reduced Graphene Oxide for Electrochemical Detection of a Stress Biomarker

A Simple, sensitive, and selective electrochemical aptasensor for cortisol based on rGO‐AuNPs

L-cysteine/MoS2 modified robust surface plasmon resonance optical fiber sensor for sensing of Ferritin and IgG

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