A flavanone derivative from the Asian medicinal herb ( Perilla frutescens ) potently suppresses IgE-mediated immediate hypersensitivity reactions

Kamei, Rikiya; Fujimura, Takashi; Matsuda, Masahisa; Kakihara, Kotaro; Hirakawa, Naoya; Baba, Kenji; Ono, Kazuhisa; Arakawa, Kenji; Kawamoto, Seiji

doi:10.1016/j.bbrc.2016.12.083

Cited by 32 publications

(28 citation statements)

References 18 publications

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“…Perilla is a widely used medicinal plant and is grown in South-east Asian countries. It has been applied for the treatment of human diseases including asthma, colds, and allergies 25 . Rosmarinic acid is the main phenolic and phytochemical compound in PLE.…”

Section: Discussionmentioning

confidence: 99%

Preventive effects of polyphenol-rich perilla leaves on oxidative stress and haemolysis

Tipsuwan¹,

Chaiwangyen²

2018

ScienceAsia

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We investigated the effect of perilla leaf extract (PLE) containing polyphenols on various biological functions including antioxidant activity, iron chelation, and red blood cell haemolysis protection. The total phenolic and flavonoid content were determined by colorimetric assay and HPLC analysis. The antioxidant activity was determined by DPPH and ABTS assay. The iron chelating effect was examined by the colorimetric method. Free radical scavenging activity was determined by haemoglobin-induced lipid peroxidation in a linoleic acid model system. Reactive oxygen species (ROS) inhibition was investigated in peripheral blood mononuclear cells. The antihaemolytic activity by AAPHinduced free radicals. The results revealed that PLE contained high levels of total phenolic compounds and total flavonoids (mainly rosmarinic acid). The IC 50 values of PLE by DPPH and ABTS radical scavenging assay were 34.3 ± 3.4 and 9.69 ± 0.14 µg/ml, respectively. The iron-binding effect of PLE was exhibited by complex formation with Fe 3+-NTA. The iron-chelating activity displayed a moderate IC 50 value of 10.1 ± 0.6 mg/ml. The PLE concentration of 200 µg/ml, significantly inhibited intracellular ROS 45%. The scavenging activity of PLE in haemoglobin-induced lipid peroxidation resulted in 27% inhibition. PLE also prevented AAPH-induced RBC haemolysis. In conclusion, PLE containing phenolic compounds plays a vital role in free radical scavenging and iron-chelating activities and subsequently prevents lipid peroxidation resulting from RBC haemolysis due to oxidative stress. Perilla products may be developed as health supplements, which could probably be used in prophylaxis and treatment of non-communicable diseases.

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Section: Discussionmentioning

confidence: 99%

Preventive effects of polyphenol-rich perilla leaves on oxidative stress and haemolysis

Tipsuwan¹,

Chaiwangyen²

2018

ScienceAsia

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“…Mucosal immunotherapy is therefore an ideal choice based on these considerations. The health-promoting functions of some phytochemical constituents are receiving increasing attention with many studies revealing that their daily intake contributes to reducing the number of patients with diseases such as allergy, arteriosclerosis, and cancer [5][6][7]. Some of the immunemodulating effects might be achieved through enhancement of duction of several types of cytokines, including IL-4, IL-17, and IFN-γ, by antigen-stimulated splenocytes.…”

Section: Introductionmentioning

confidence: 99%

Cyclopia Extracts Enhance Th1-, Th2-, and Th17-type T Cell Responses and Induce Foxp3+ Cells in Murine Cell Culture

et al. 2017

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, one of the traditional South African medicinal plants, and other species of the same genus offer noteworthy phenolic profiles, in particular high levels of the anti-allergic xanthone mangiferin. Hot water and 40% ethanol-water (v/v) extracts, prepared from , and were tested for immune-regulating activity using murine splenocytes and mesenteric lymph node cells. The 40% ethanol-water extracts of significantly enhanced production of several types of cytokines, including IL-4, IL-17, and IFN-, by antigen-stimulated splenocytes. A concentration-dependent response was observed, noticeably for IFN- production. The activity of the extracts did not correlate with the content of any of the major phenolic compounds, indicative that other extract constituents also play a role in immunomodulation. Additionally, the increased ratio of CD4CD25Foxp3 Treg cells to total CD4 cells indicated induction of Foxp3 cells when mesenteric lymph node cells were cultured in the presence of these two extracts. This study is the first reporting immunostimulatory activity for , which are widely consumed as the herbal tea known as honeybush, underpinning further investigations into the potential use of its extracts as adjuvants for mucosal immunotherapy.

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“…The elevation of Ca 2+ is a critical process for MC secretory granule translocation [ 33 ]. As a calcium ionophore, A23187 is often used to study the status of intracellular Ca 2+ influx [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Viridicatol Isolated from Deep-Sea Penicillium Griseofulvum Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation

et al. 2020

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Viridicatol is a quinoline alkaloid isolated from the deep-sea-derived fungus Penicillium griseofulvum. The structure of viridicatol was unambiguously established by X-ray diffraction analysis. In this study, a mouse model of ovalbumin-induced food allergy and the rat basophil leukemia (RBL)-2H3 cell model were established to explore the anti-allergic properties of viridicatol. On the basis of the mouse model, we found viridicatol to alleviate the allergy symptoms; decrease the levels of specific immunoglobulin E, mast cell protease-1, histamine, and tumor necrosis factor-α; and promote the production of interleukin-10 in the serum. The treatment of viridicatol also downregulated the population of B cells and mast cells (MCs), as well as upregulated the population of regulatory T cells in the spleen. Moreover, viridicatol alleviated intestinal villi injury and inhibited the degranulation of intestinal MCs to promote intestinal barrier repair in mice. Furthermore, the accumulation of Ca2+ in RBL-2H3 cells was significantly suppressed by viridicatol, which could block the activation of MCs. Taken together, these data indicated that deep-sea viridicatol may represent a novel therapeutic for allergic diseases.

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A flavanone derivative from the Asian medicinal herb ( Perilla frutescens ) potently suppresses IgE-mediated immediate hypersensitivity reactions

Cited by 32 publications

References 18 publications

Preventive effects of polyphenol-rich perilla leaves on oxidative stress and haemolysis

Preventive effects of polyphenol-rich perilla leaves on oxidative stress and haemolysis

Cyclopia Extracts Enhance Th1-, Th2-, and Th17-type T Cell Responses and Induce Foxp3+ Cells in Murine Cell Culture

Viridicatol Isolated from Deep-Sea Penicillium Griseofulvum Alleviates Anaphylaxis and Repairs the Intestinal Barrier in Mice by Suppressing Mast Cell Activation

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