A fiber optic biosensor for specific identification of dead Escherichia coli O157:H7

Liu, Ting; Zhao, Yong; Zhang, Zhonghuan; Zhang, Pingping; Yang, Ruifu; Yang, Changxi; Zhou, Lei

doi:10.1016/j.snb.2014.02.003

Cited by 14 publications

(10 citation statements)

References 37 publications

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“…Then, the fluorescence signal was injected into an avalanche photo-diode (APD, LCSA500-01, Laser Components, Germany) for photo-electronic conversion, and detection in data acquisition (DAQ) system. To enhance signal resolution, digital signals from DAQ system were further processed by a correlation detection system based on phase-locked filtering algorithm, which was performed with LabVIEW (Liu et al, 2014). Finally, the fluorescence signal was displayed on the computer screen.…”

Section: Experimental Principlementioning

confidence: 99%

“…A taper-and-cylinder combination optical fiber was prepared as the fiber probe. It was fabricated with a simple static tube etching method with 40% hydrofluoric acid (HF) as the etchant (Liu et al, 2014;Zhang et al, 2014). The fiber probe (inset in Figure 1A) consisted of a tapered section and a sensing region, whose length were approximately 0.03 and 1 cm, respectively.…”

Section: Preparation Of the Antibody-immobilized Optical Fiber Probes Of The Biosensormentioning

confidence: 99%

“…We developed an optical fiber probe-based immunofluorescence biosensor that combined the advantages of antigen-antibody specificity, fluorescence labeling and transmission of fluorescence signals along the optical fiber. The surface of the probe of the biosensor was immobilized with Escherichia coli-specific antibodies, and the detection sensitivity for E. coli reached 10 3 CFU/ml (CFU, colony forming units, an estimate of viable bacterial numbers usually existing in the form of colony), with high specificity (Liu et al, 2014;Zhang et al, 2014). However, the common fluorescence labelling techniques applied in their biosensor still have disadvantages, such as short fluorescence lifetime and spectral overlap affecting detection accuracy (Pu and Liu, 2009).…”

Section: Introductionmentioning

confidence: 99%

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A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

Cui

Zhou

Liu

et al. 2021

Front. Cell. Infect. Microbiol.

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Staphylococcus aureus (S. aureus) is one of the most common clinical pathogenic bacteria with strong pathogenicity and usually leads to various suppurative infections with high fatality. Traditional bacterial culture for the detection of S. aureus is prone to diagnosis and antimicrobial treatment delays because of its long-time consumption and low sensitivity. In this study, we successfully developed a quantum dots immunofluorescence biosensor for S. aureus detection. The biosensor combined the advantages of biosensors with the high specificity of antigen-antibody immune interactions and the high sensitivity and stability of quantum dots fluorescence. The results demonstrated that the biosensor possessed high specificity and high sensitivity for S. aureus detection. The detection limit of S. aureus reached 1 × 104 CFU/ml or even 1 × 103 CFU/ml, and moreover, the fluorescence intensity had a significant positive linear correlation relationship with the logarithm of the S. aureus concentration in the range of 103–107 CFU/ml (correlation coefficient R2 = 0.9731, P = 0.011). A specificity experiment showed that this biosensor could effectively distinguish S. aureus (1 × 104 CFU/ml and above) from other common pathogenic (non-S. aureus) bacteria in nosocomial infections, such as Klebsiella pneumoniae, Pseudomonas aeruginosa, Acinetobacter baumannii and Escherichia coli. Additionally, the whole detection procedure spent only 2 h. In addition, the biosensor in this study may not be affected by the interference of the biofilm or other secretions since the clinical biological specimens are need to be fully liquefied to digest and dissolve viscous secretions such as biofilms before the detection procedure of the biosensor in this study. In conclusion, the biosensor could meet the need for rapid and accurate S. aureus detection for clinical application.

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Section: Experimental Principlementioning

confidence: 99%

Section: Preparation Of the Antibody-immobilized Optical Fiber Probes Of The Biosensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

Cui

Zhou

Liu

et al. 2021

Front. Cell. Infect. Microbiol.

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“…A useful and commercially attractive sensor systems for accurate and rapid detection of biological and chemical threat agents has to satisfy some important properties [ 27 ]. Various recent studies indicate that plasmonic sensors can be a key platform for monitoring biological and chemical threat agents owing to their combination of different charming properties such as sensitivity, rapid, unlabeled, low cost, real time, and portability [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

Plasmonic Sensors for Monitoring Biological and Chemical Threat Agents

2020

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Sensors are excellent options owing to their ability to figure out a large number of problems and challenges in several areas, including homeland security, defense, medicine, pharmacology, industry, environment, agriculture, food safety, and so on. Plasmonic sensors are used as detection devices that have important properties, such as rapid recognition, real-time analysis, no need labels, sensitive and selective sensing, portability, and, more importantly, simplicity in identifying target analytes. This review summarizes the state-of-art molecular recognition of biological and chemical threat agents. For this purpose, the principle of the plasmonic sensor is briefly explained and then the use of plasmonic sensors in the monitoring of a broad range of biological and chemical threat agents is extensively discussed with different types of threats according to the latest literature. A conclusion and future perspectives are added at the end of the review.

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“…In the last decades, several studies concerning optical fiber sensors have arisen and many optical fiber biosensor techniques are still being published [ 2 , 3 , 4 , 5 ]. Biosensors that employ antibodies as the biological element of recognition, responsible for their specificity, are called immunosensors.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results

Rodrigues

Lopes

Franco³

et al. 2017

Sensors

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Conventional pathogen detection methods require trained personnel, specialized laboratories and can take days to provide a result. Thus, portable biosensors with rapid detection response are vital for the current needs for in-loco quality assays. In this work the authors analyze the characteristics of an immunosensor based on the evanescent field in plastic optical fibers with macro curvature by comparing experimental with simulated results. The work studies different shapes of evanescent-wave based fiber optic sensors, adopting a computational modeling to evaluate the probes with the best sensitivity. The simulation showed that for a U-Shaped sensor, the best results can be achieved with a sensor of 980 µm diameter by 5.0 mm in curvature for refractive index sensing, whereas the meander-shaped sensor with 250 μm in diameter with radius of curvature of 1.5 mm, showed better sensitivity for either bacteria and refractive index (RI) sensing. Then, an immunosensor was developed, firstly to measure refractive index and after that, functionalized to detect Escherichia coli. Based on the results with the simulation, we conducted studies with a real sensor for RI measurements and for Escherichia coli detection aiming to establish the best diameter and curvature radius in order to obtain an optimized sensor. On comparing the experimental results with predictions made from the modelling, good agreements were obtained. The simulations performed allowed the evaluation of new geometric configurations of biosensors that can be easily constructed and that promise improved sensitivity.

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A fiber optic biosensor for specific identification of dead Escherichia coli O157:H7

Cited by 14 publications

References 37 publications

A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

A New Optical Fiber Probe-Based Quantum Dots Immunofluorescence Biosensors in the Detection of Staphylococcus aureus

Plasmonic Sensors for Monitoring Biological and Chemical Threat Agents

Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results

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