A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets

Arnaldi, Pietro; Lisa, Donatella Di; Maddalena, Lorenza; Carosio, Federico; Fina, Alberto; Pastorino, Laura; Monticelli, Orietta

doi:10.1016/j.carbpol.2021.118420

Cited by 16 publications

(12 citation statements)

References 33 publications

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“…For the design of ECM-based scaffolds, numerous substrates have been successfully developed, including biopolymers [93,264], agarose [265], collagen [88,266], chitosan [267,268], silk proteins [269] and gel-like substances [267]. The majority of them are biocompatible polymer gels or solid porous matrices, that can be further coated with specific ECM components to support cell development, and guide axons-dendrites outgrowth [270].…”

Section: D Rodent Neuronal Cultures On Measmentioning

confidence: 99%

The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research

2023

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In vitro neuronal models have become an important tool to study healthy and diseased neuronal circuits. The growing interest of neuroscientists to explore the dynamics of neuronal systems and the increasing need to observe, measure and manipulate not only single neurons but populations of cells pushed for technological advancement. In this sense, Micro-Electrode Arrays (MEAs) emerged as a promising technique, made of cell culture dishes with embedded micro-electrodes allowing non-invasive and relatively simple measurement of the activity of neuronal cultures at the network level.In the past decade, MEAs popularity has rapidly grown. MEAs devices have been extensively used to measure the activity of neuronal cultures mainly derived from rodents. Rodent neuronal cultures on MEAs have been employed to investigate physiological mechanisms, study the effect of chemicals in neurotoxicity screenings, and model the electrophysiological phenotype of neuronal networks in different pathological conditions. With the advancements in human induced pluripotent stem cells (hiPSCs) technology, the differentiation of human neurons from the cells of adult donors became possible. hiPSCs-derived neuronal networks on MEAs have been employed to develop patient-specific in vitro platforms to characterize the pathophysiological phenotype and to test drugs, paving the way towards personalized medicine.In this review, we first describe MEAs technology and the information that can be obtained from MEAs recordings. Then, we give an overview of studies in which MEAs have been used in combination with different neuronal systems (i.e., rodent 2D and 3D neuronal cultures, organotypic brain slices, hiPSCs-derived 2D and 3D neuronal cultures, and brain organoids) for biomedical research, including physiology studies, neurotoxicity screenings, disease modeling, and drug testing. We end by discussing potential, challenges and future perspectives of MEAs technology, and providing some guidance for the choice of theneuronal model and MEAs device, experimental design, data analysis and reporting for scientific publications.

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Section: D Rodent Neuronal Cultures On Measmentioning

confidence: 99%

The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research

2023

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“…Different methodologies to build 3D models have been sought with the aim to investigate neuronal functions in a more in-vivo-like condition [ 41 , 42 , 43 ]. Most of the strategies for 3D tissue construction are scaffold-based (i.e., ECM- [ 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 ] or microbeads-based [ 52 , 53 , 54 , 55 , 56 ]) in which the used materials allow the spontaneous formation of 3D networks with arborizations in the 3D space. Human iPSCs are also able to self-assemble without the use of 3D scaffolds into brain organoids, which are 3D spheroidal aggregates composed by the heterogeneous population of cells with a cytoarchitecture resembling the embryonic human brain [ 57 , 58 , 59 , 60 , 61 ].…”

Section: Introductionmentioning

confidence: 99%

Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests

et al. 2023

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With the advent of human-induced pluripotent stem cells (hiPSCs) and differentiation protocols, methods to create in-vitro human-derived neuronal networks have been proposed. Although monolayer cultures represent a valid model, adding three-dimensionality (3D) would make them more representative of an in-vivo environment. Thus, human-derived 3D structures are becoming increasingly used for in-vitro disease modeling. Achieving control over the final cell composition and investigating the exhibited electrophysiological activity is still a challenge. Thence, methodologies to create 3D structures with controlled cellular density and composition and platforms capable of measuring and characterizing the functional aspects of these samples are needed. Here, we propose a method to rapidly generate neurospheroids of human origin with control over cell composition that can be used for functional investigations. We show a characterization of the electrophysiological activity exhibited by the neurospheroids by using micro-electrode arrays (MEAs) with different types (i.e., passive, C-MOS, and 3D) and number of electrodes. Neurospheroids grown in free culture and transferred on MEAs exhibited functional activity that can be chemically and electrically modulated. Our results indicate that this model holds great potential for an in-depth study of signal transmission to drug screening and disease modeling and offers a platform for in-vitro functional testing.

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“…To develop bone substitute material with high biocompatibility and excellent osteogenic inducing capability, chitosan, a copolymer derived from chitin’s deacetylation, has been widely used as a bone scaffold because of its excellent biocompatibility and low toxicity [ 9 , 10 , 11 , 12 ]. However, the mechanical properties of these polymer scaffolds do not provide sufficient structural support [ 13 , 14 ], especially in the area needed for implant placement. To enhance the mechanical properties of chitosan for bone tissue engineering applications, several studies have tried to incorporate bio-ceramic into the chitosan scaffolds [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Epigenetic Modulation Chitosan-Based Scaffold as a Promising Bone Regenerative Material

Sukpaita

Chirachanchai

Chanamuangkon

et al. 2022

Cells

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Bone tissue engineering is a complicated field requiring concerted participation of cells, scaffolds, and osteoactive molecules to replace damaged bone. This study synthesized a chitosan-based (CS) scaffold incorporated with trichostatin A (TSA), an epigenetic modifier molecule, to achieve promising bone regeneration potential. The scaffolds with various biphasic calcium phosphate (BCP) proportions: 0%, 10%, 20%, and 40% were fabricated. The addition of BCP improved the scaffolds’ mechanical properties and delayed the degradation rate, whereas 20% BCP scaffold matched the appropriate scaffold requirements. The proper concentration of TSA was also validated. Our developed scaffold released TSA and sustained them for up to three days. The scaffold with 800 nM of TSA showed excellent biocompatibility and induced robust osteoblast-related gene expression in the primary human periodontal ligament cells (hPDLCs). To evaluate in vivo bone regeneration potential, the scaffolds were implanted in the mice calvarial defect model. The excellent bone regeneration ability was further demonstrated in the micro-CT and histology sections compared to both negative control and commercial bone graft product. New bone formed in the CS/BCP/TSA group revealed a trabeculae-liked characteristic of the mature bone as early as six weeks. The CS/BCP/TSA scaffold is an up-and-coming candidate for the bone tissue engineering scaffold.

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A facile approach for the development of high mechanical strength 3D neuronal network scaffold based on chitosan and graphite nanoplatelets

Cited by 16 publications

References 33 publications

The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research

The potential of in vitro neuronal networks cultured on micro electrode arrays for biomedical research

Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests

Novel Epigenetic Modulation Chitosan-Based Scaffold as a Promising Bone Regenerative Material

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