A Escola De Enfermagem Da Universidade De São Paulo

Lacaz, Carlos da Silva

doi:10.1590/0080-6234198101500100003

Cited by 10 publications

(26 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This mycosis occurs predominantly in Brazil, which accounts for 295 of a total of 458 cases of the disease 17 , and affects mainly rural workers that live in constant contact with vegetation and soil, such as the rubber tree tappers from the Amazon 12,17 .…”

Section: Introductionmentioning

confidence: 99%

“…The fungus reproduces by single or multiple budding, presenting a chain-like arrangement in which individual cells are interconnected by tube-like structures 12 .…”

Section: Introductionmentioning

confidence: 99%

“…One peculiarity of L. loboi is the fact that the fungus has not been cultured in artificial media so far despite numerous attempts 8,12,18 . However, the development of an experimental model of Jorge Lobo's disease in BALB/c mice, which show histological alterations similar to the human disease with a large number of viable fungi 14 , has contributed to the study of this mycosis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vitro and in situ activation of the complement system by the fungus Lacazia loboi

Vilani-Moreno

Mozer

Sene

et al. 2007

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYSince there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 o C. Next, the tubes were incubated for 2 h with a pool of noninactivated AB + serum, inactivated serum, serum diluted in EGTA-MgCl 2 , and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi.

show abstract

Section: Introductionmentioning

confidence: 99%

“…The fungus reproduces by single or multiple budding, presenting a chain-like arrangement in which individual cells are interconnected by tube-like structures 12 .…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro and in situ activation of the complement system by the fungus Lacazia loboi

Vilani-Moreno

Mozer

Sene

et al. 2007

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

show abstract

“…10 Isso sugere a existência do componente genético, somado aos fatores ambientais e comportamentais, atuando na ocorrência da doença nessa população. Sabe-se que nesta região existe uma predisposição a infecções por fungos causadores de micoses sistêmicas.…”

Section: Discussionunclassified

Distribuição fenotípica e a freqüência genotípica do sistema sangüíneo Duffy em pacientes com a doença de Jorge Lobo

Silva¹,

Rosa²,

Belone³

2006

Rev. Bras. Hematol. Hemoter.

View full text Add to dashboard Cite

incubando-se a 37ºC e aplicando-se o teste da antiglobulina humana. Os resultados mostram que a distribuição fenotípica do sistema Duffy nos pacientes difere significativamente daquela observada nos controles, pela alta freqüência de indivíduos com fenótipo Fy(a-b-). Observase claramente que os pacientes apresentam maior freqüência do alelo FY e menor do alelo FYA do que os controles. O alelo FYB (não inclui os alelos que não são expressos) tem praticamente a mesma freqüência nos dois grupos. Com relação à comparação entre distribuição do grupo sangüíneo Duffy com as formas clínicas, sugere maior número de pacientes com forma localizada da doença de Jorge Lobo apresentando um predomínio do fenótipo Fy(a-b+) seguido do fenótipo Fy(a-b-) e inexistência do fenótipo Fy(a+b-). Por outro lado, temos a forma disseminada com maior incidência de fenótipo Fy(a+b-). A forma multifocal foi menos expressiva. Desse modo, podemos inferir uma provável prevalência de fenótipo Duffy negativo na região por nós estudada e os poucos dados disponíveis até o momento tornam clara a necessidade de elaborar tabelas de freqüências fenotípicas para a população acometida pela doença de JorgeLobo, bem como realizar pesquisas para melhor entendimento da função biológica dos antígenos do sistema Duffy. Rev. bras. hematol. hemoter. 2006;28(4):264-268. Palavras-chave: Fenótipo Duffy; doença de Jorge Lobo; Lacazia loboi.Distribuição fenotípica e a freqüência genotípica do sistema sangüíneo Duffy em pacientes com a doença de Jorge Lobo Phenotypic distribution and genotypic frequency of the Duffy blood group system in Jorge Lobo's disease patients Introdução A doença, descrita por Jorge de Oliveira Lobo 1 como uma micose cutânea-subcutânea de evolução crônica, causada pelo fungo Lacazia loboi (L. loboi) acomete, principalmente, trabalhadores rurais em contato com o solo e a vegetação, como é o caso dos seringueiros da Amazônia.

show abstract

“…The disease has also been described to occur in other Latin American countries 7,8,14,20,21 . A keloid-like lesion on the skin is the foremost sign of the disease 10 and the etiological agent, Lacazia loboi (L. loboi) 1 , is found in large numbers inside macrophages.…”

Section: Introductionmentioning

confidence: 99%

Inoculation of Lacazia loboi into the subcutaneous tissue of the hamster cheek pouch

Opromolla

Nogueira

2000

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYThe subcutaneous tissue of the hamster cheek pouch, a site of immunologic privilege, has been used to investigate the potential infectivity of different types of parasites. It has been demonstrated that the implantation of fragments of lesions induced by the fungus Lacazia loboi, the etiologic agent of Jorge Lobo's disease, into the subcutaneous tissue of the hamster cheek pouch resulted in parasite multiplication and dissemination to satellite lymph nodes 16 . Here we describe the evolution of lesions induced by the inoculation of the isolated fungus into this immunologically privileged site. The morphology of the inflammatory response and fungal viability and proliferation were evaluated. Inoculation of the fungus into the cheek pouch induced histiocytic granulomas with rare lymphocytes. Although fungal cells were detected for a period of up to 180 days in these lesions, the fungi lost viability after the first day of inoculation. In contrast, when the parasite was inoculated into the footpad, non-organized histiocytic lesions were observed. Langhan's giant cells, lymphocytes and fungal particles were observed in these lesions. Fungal viability was observed up to 60 days after inoculation and non-viable parasites were present in the persistent lesions up to 180 days post-inoculation. These data indicate that the subcutaneous tissue of the hamster cheek pouch is not a suitable site for the proliferation of Lacazia loboi when the fungus isolated from human tissues is tested.

show abstract

A Escola De Enfermagem Da Universidade De São Paulo

Cited by 10 publications

References 0 publications

In vitro and in situ activation of the complement system by the fungus Lacazia loboi

In vitro and in situ activation of the complement system by the fungus Lacazia loboi

Distribuição fenotípica e a freqüência genotípica do sistema sangüíneo Duffy em pacientes com a doença de Jorge Lobo

Inoculation of Lacazia loboi into the subcutaneous tissue of the hamster cheek pouch

Contact Info

Product

Resources

About