Inoculation of Lacazia loboi into the subcutaneous tissue of the hamster cheek pouch

Abstract

SUMMARYThe subcutaneous tissue of the hamster cheek pouch, a site of immunologic privilege, has been used to investigate the potential infectivity of different types of parasites. It has been demonstrated that the implantation of fragments of lesions induced by the fungus Lacazia loboi, the etiologic agent of Jorge Lobo's disease, into the subcutaneous tissue of the hamster cheek pouch resulted in parasite multiplication and dissemination to satellite lymph nodes 16 . Here we describe the evolution of lesions induced by the inoculation of the isolated fungus into this immunologically privileged site. The morphology of the inflammatory response and fungal viability and proliferation were evaluated. Inoculation of the fungus into the cheek pouch induced histiocytic granulomas with rare lymphocytes. Although fungal cells were detected for a period of up to 180 days in these lesions, the fungi lost viability after the first day of inoculation. In contrast, when the parasite was inoculated into the footpad, non-organized histiocytic lesions were observed. Langhan's giant cells, lymphocytes and fungal particles were observed in these lesions. Fungal viability was observed up to 60 days after inoculation and non-viable parasites were present in the persistent lesions up to 180 days post-inoculation. These data indicate that the subcutaneous tissue of the hamster cheek pouch is not a suitable site for the proliferation of Lacazia loboi when the fungus isolated from human tissues is tested.

Lacaziose (doença de Jorge Lobo): revisão e atualização

“…Sampaio e Dias 52 inocularam na bolsa jugal de 22 hamsters (M. auratus), produzindo nódulos com resposta histiociticogigantocitária e numerosos fungos, também em tatu (E. sexcinctus), 53 em quelônios, 54 e tentativas de cultura do patógeno, 55 sem sucesso. Experimentações em animais de laboratório foram também efetuadas por Opromolla et al 56,57 e Madeira et al 58…”

Modeling lacaziosis lesion progression in common bottlenose dolphins Tursiops truncatus using long-term photographic records

“…As a result, very little is understood about the conditions under which the pathogen grows and thrives, as well as its source and transmission route (Wiersema & Niemel 1965, Pang et al 2004, Lupi et al 2005, Paniz-Mondolfi et al 2007). Experimental inoculation attempts have been made using guinea pigs (Wiersema & Niemel 1965), hamsters (Wiersema & Niemel 1965, Opromolla & Noguiera 2000, tortoises (Geochelone denticulate, G. carbonaria and Kinosternon scorpioides; Sampaio et al 1971), monkeys (Macacca mulatta, M. nemestrina and M. fascicularis; Caldwell et al 1975), armadillos Euphractus sexcinctus (Sampaio & Braga-Dias 1977) and mice (Wiersema & Niemel 1965, Caldwell et al 1975, Opromolla et al 1999, Madeira et al 2000, 2003, Belone et al 2003, but many of those attempts yielded only short-term data on the development of the disease. Opromolla et al (1999) were able to successfully inoculate a Swiss strain of mice with lacaziosis and follow disease progression up to 18 mo post-inoculation; however, clinical presentation of the disease did not occur, and very few fungal cells were active by the end of the study.…”

Análise comparativa do perfil citocínico Th22 na imunopatogenia da doença de Jorge Lobo e cromomicose

“…Separando a epiderme do infiltrado inflamatório, observamos uma faixa grenz de espessura variável. Na derme há vasos dilatados e infiltrado inflamatório granulomatoso, nodular e difuso, constituído, principalmente, por macrófagos, histiócitos, células gigantes multinucleadas do tipo Langhans e do tipo corpo estranho com citoplasma repleto de parasitos e, em menor quantidade, eosinófilos e plasmócitos Nogueira, 2000).…”