A dual-site fluorescent probe for direct and highly selective detection of cysteine and its application in living cells

Novel dual-site fluorescent probe for monitoring cysteine and sulfite in living cells

“…Based on our previous findings and other reports, 31,34 a mechanism for the response of the LC-1 probe to Cys and SO 3 2− was derived, and is shown in Scheme 2 . The mechanism of the response of LC-1 to Cys was proposed to be based on the nucleophilic addition of the thiol in Cys to the α,β-unsaturated ketone in LC-1 to form a thioether exhibiting blue fluorescence under excitation at 320 nm.…”

“… 26–30 Our group previously designed and synthesized several fluorescent probes for sensitively and selectively detecting Cys over other biothiols in living cells and mice. 31–33 …”

Fluorescent probes for detecting cysteine

“…The double-sites probes have more functions and good fluorescence performances drawing the attention of researchers. Wang et al [78] synthesized probe 52 that shows different fluorescent properties at diverse concentrations of Cys, which depends on the structure of 52 with fast and slow response sites for Cys. 52 contains not only intramolecular hydrogen bond for changing properties but also acrylate (fast site) and α, β-unsaturated ketone (slow site) as response parts leads to turn-on and ratiometric fluorescence (figure 50).…”

Anti-aggregation colorimetric sensing of cysteine using silver nanoparticles in the presence of Pb2+

“…3 Therefore, the detection of cysteine is essential. Various analytical methods for determining cysteine in biological and pharmaceutical samples include biochemical analysis, 4 high-performance liquid chromatography, 5 fluorescence, 6 mass spectrometry, 7 chemiluminescence, 8 and electrochemistry. 9 These assays are widely used to determine cysteine; however, they are accompanied by drawbacks such as heavy consumption of chemicals, use of sophisticated equipment, and complex procedures.…”