A dual-response fluorescent probe for discriminative sensing of hydrazine and bisulfite as well as intracellular imaging with different emission

Yang, Yu; Qing, Min; Luo, Xiao Ye; Xie, Juan; Zhang, Li Na

doi:10.1016/j.saa.2021.120795

Cited by 15 publications

(6 citation statements)

References 33 publications

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“…To detect toxic analytes, the 1,1,2-trimethyl-1 H -benzo(e)indole moiety was mainly used to create a higher wavelength-emitting fluorescent probe. When the benzo-hemicyanine dye was coupled with an electron-donating system, it yields active CN + and CC bonds in conjugation due to the electron-deficient character of the alkylated nitrogen atom. , The active CC double bond is expected to be disconnected (loss of conjugation) by the addition of HS – , resulting in a noticeable blue shift; − however, a nonfluorescence emission was observed due to the nucleophilic addition of cyanide to the electron-deficient imine bond on the benzo-hemicyanine moiety. − Based on this, we chose the 1,1,2-trimethyl-1 H -benzo(e)indole moiety with N -alkylation by the propyl group to avoid interactions of common ions and fused with 4-(methylthio)benzaldehyde. The presence of the electron-rich sulfur atom in 4-(methylthio)benzaldehyde and electron-deficient nature of benzo-hemicyanine facilitate donor-π-acceptor (push–pull) interactions between polar CC double bonds.…”

Section: Resultsmentioning

confidence: 99%

Dual-Responsive Benzo-Hemicyanine-Based Fluorescent Probe for Detection of Cyanide and Hydrogen Sulfide: Real-Time Application in Identification of Food Spoilage

Magesh

Vijay

et al. 2023

J. Agric. Food Chem.

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Colorimetric and fluorescent probes have received a lot of attention for detecting lethal analytes in realistic systems and in living things. Herein, a dual-approachable Benzo-hemicyaninebased red-emitting fluorescent probe PBiSMe, for distinct and instantaneous detection of CN– and HS– was synthesized. The PBiSMe emitted red fluorescence (570 nm) can switch to turn-off (570 nm) and blue fluorescence (465 nm) in response to CN– and HS–, respectively. Other nucleophilic reagents, such as reactive sulfur species (RSS) and anions, have no contact or interference with the probe; instead, a unique approach is undertaken to exclusively interact with CN– and HS– over a wide pH range. The measured detection limits for CN– (0.43 μM) and HS– (0.22 μM) ions are lower than the World Health Organization’s (WHO) recommended levels in drinking water. We confirmed 1:1 stoichiometry ratio using Job’s plot and observed good quantum yield for both analytes. The probe-coated paper strips were used to detect the H2S gas produced by food spoilage (such as eggs, raw meat, and fish) via an eye-catching visual response. Moreover, fluorescence bioimaging studies of living cells was done to confirm the probe’s potential by monitoring the presence of CN– and HS– in a living system.

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Section: Resultsmentioning

confidence: 99%

Dual-Responsive Benzo-Hemicyanine-Based Fluorescent Probe for Detection of Cyanide and Hydrogen Sulfide: Real-Time Application in Identification of Food Spoilage

Magesh

Vijay

et al. 2023

J. Agric. Food Chem.

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“…Moreover, these fluorochromes can flow between adjacent membranes, leading to increased fluorescence and false-positive results during evaluation. Dual or discriminative immunofluorescence allows determination of the location where the fluorescence signal is occurring, whether in the cytoplasm, endosome, or another organelle ( Yang et al, 2022 ). However, this approach uses statistical methods to quantify a region observed through the microscope.…”

Section: Discussionmentioning

confidence: 99%

Exploring the secrets of virus entry: the first respiratory syncytial virus carrying beta lactamase

De Ávila-Arias,

Villarreal-Camacho,

Cadena-Cruz

et al. 2024

Front. Microbiol.

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BackgroundRespiratory Syncytial Virus (RSV) presents a significant health threat, especially to young children. In-depth understanding of RSV entry mechanisms is essential for effective antiviral development. This study introduces an innovative RSV variant, featuring the fusion of the beta-lactamase (BlaM) enzyme with the RSV-P phosphoprotein, providing a versatile tool for dissecting viral entry dynamics.MethodsUsing the AlphaFold2 algorithm, we modeled the tertiary structure of the P-BlaM chimera, revealing structural similarities with both RSV-P and BlaM. Functional assessments, utilizing flow cytometry, quantified beta-lactamase activity and GFP expression in infected bronchial epithelial cells. Western blot analysis confirmed the integrity of P-BlaM within virions.ResultsThe modeled P-BlaM chimera exhibited structural parallels with RSV-P and BlaM. Functional assays demonstrated robust beta-lactamase activity in recombinant virions, confirming successful P-BlaM incorporation as a structural protein. Quercetin, known for its antiviral properties, impeded viral entry by affecting virion fusion. Additionally, Ulixertinib, an ERK-1/2 inhibitor, significantly curtailed viral entry, implicating ERK-1/2 pathway signaling.ConclusionsOur engineered RSV-P-BlaM chimera emerges as a valuable tool, illuminating RSV entry mechanisms. Structural and functional analyses unveil potential therapeutic targets. Quercetin and Ulixertinib, identified as distinct stage inhibitors, show promise for targeted antiviral strategies. Time-of-addition assays pinpoint quercetin’s specific interference stage, advancing our comprehension of RSV entry and guiding future antiviral developments.

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“…7 In order to selectively detect analytes that are important to both ecology and physiology, so far, many research groups have reported ESIPT-based fluorophores. [8][9][10] In the recent past, some examples were reported in judicially designed molecules where two-way double proton transfer sites were incorporated to understand the competitiveness of PT processes. [11][12][13] Some research groups have started to investigate the molecular behaviours and photophysical properties of ESIPT chromophores with an extra competitive proton acceptor site both in the solution and solid phases.…”

Section: Introductionmentioning

confidence: 99%

Switching of photoinduced proton transfer from one six-membered hydrogen-bonded ring to other: a molecule of hydrazine and pH sensor

Bhakta,

Guchhait

2024

Phys. Chem. Chem. Phys.

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A dual-response fluorescent probe for discriminative sensing of hydrazine and bisulfite as well as intracellular imaging with different emission

Cited by 15 publications

References 33 publications

Dual-Responsive Benzo-Hemicyanine-Based Fluorescent Probe for Detection of Cyanide and Hydrogen Sulfide: Real-Time Application in Identification of Food Spoilage

Dual-Responsive Benzo-Hemicyanine-Based Fluorescent Probe for Detection of Cyanide and Hydrogen Sulfide: Real-Time Application in Identification of Food Spoilage

Exploring the secrets of virus entry: the first respiratory syncytial virus carrying beta lactamase

Switching of photoinduced proton transfer from one six-membered hydrogen-bonded ring to other: a molecule of hydrazine and pH sensor

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