A dual-function oligonucleotide-based ratiometric fluorescence sensor for ATP detection

Fan, Yao-Yao; Deng, Xu; Wang, Man; Li, Jun; Zhang, Zhiqi

doi:10.1016/j.talanta.2020.121349

Cited by 25 publications

(8 citation statements)

References 48 publications

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“…The limit of detection of the ECL-RET biosensor was measured with the commonly-used 3σ method ( Yang et al., 2020 ; Fan et al., 2020 ; Liang et al., 2016 ; Cai et al., 2021 ). Briefly, for each condition, the standard deviation of samples with the lowest concentration is calculated and multiply by three to obtain the 3σ limit of detection.…”

Section: Methodsmentioning

confidence: 99%

An electrochemiluminescence resonance energy transfer biosensor for the detection of circulating tumor DNA from blood plasma

et al. 2021

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Summary A liquid biopsy is a noninvasive approach for detecting double-stranded circulating tumor DNA (ctDNA) of 90–320 nucleotides in blood plasma from patients with cancer. Most techniques employed for ctDNA detection are time consuming and require expensive DNA purification kits. Electrochemiluminescence resonance energy transfer (ECL-RET) biosensors exhibit high sensitivity, a wide response range, and are promising for straightforward sensing applications. Until now, ECL-RET biosensors have been designed for sensing short single-stranded oligonucleotides of less than 45 nucleotides. In this work, an ECL-RET biosensor comprising graphitic carbon nitride quantum dots was assessed for the amplification-free detection in the blood plasma of DNA molecules coding for the EGFR L858R mutation, which is associated with non-small-cell lung cancer. Following a low-cost pre-treatment, the highly specific ECL-RET biosensor quantified double-stranded EGFR L858R DNA of 159 nucleotides diluted into the blood within a linear range of 0.01 fM to 1 pM, demonstrating its potential for noninvasive biopsies.

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Section: Methodsmentioning

confidence: 99%

An electrochemiluminescence resonance energy transfer biosensor for the detection of circulating tumor DNA from blood plasma

et al. 2021

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“…However, the reliability of this detection strategy, which relies on a single fluorophore, is easily disturbed by unstable factors such as the light source and complex solution environment [ 32 , 33 ]. To overcome this limitation, ratiometric fluorescence biosensing has received increasing attention due to its capability to counteract the effects of instabilities [ 34 , 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Ratiometric Fluorescence Detection of Colorectal Cancer-Associated Exosomal miR-92a-3p with DSN-Assisted Signal Amplification by a MWCNTs@Au NCs Nanoplatform

Sun

Tong

et al. 2022

Biosensors

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The detection of miRNA shows great promise in disease diagnosis. In this work, a ratiometric fluorescent biosensor based on multi-walled carbon nanotubes@gold nanoclusters (MWCNTs@Au NCs) and duplex-specific nuclease (DSN)-assisted signal amplification was fabricated for miRNA detection. Colorectal cancer (CRC)-associated miR-92a-3p extracted from exosomes was selected as the target. MWCNTs@Au NCs performs the dual functions of fluorescence quencher and internal fluorescence reference. In the absence of miR-92a-3p, an Atto-425-modified single-stranded DNA probe is adsorbed on MWCNTs@Au NCs, resulting in the quenching of Atto-425. In the presence of miR-92a-3p, the duplex is formed by hybridization of the probe and miR-92a-3p and leaves the MWCNTs@Au NCs, resulting in the fluorescence recovery of Atto-425. DSN can cleave the probe and result in the release of miR-92a-3p. The released miR-92a-3p can hybridize with other probes to form a signal amplification cycle. The fluorescence of MWCNTs@Au NCs remains stable and constitutes a ratiometric fluorescence system with that of Atto-425. A detection concentration interval of 0.1–10 pM and a limit of detection of 31 fM was obtained under optimized measurement conditions. In addition, the accuracy of the biosensor was validated by detecting the concentration of miR-92a-3p extracted from clinical exosome samples.

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“…The aptamers can be functional for native conformations of target molecules on live cells, so cell surface transmembrane proteins can be considered as targets [3][4][5][6]. The 3D folded structure permits the formation of stable complexes with various targets, such as proteins, nucleic acids, and small molecules [7][8][9][10][11][12][13]. Compared to antibodies, these aptamers are characterized by numerous advantages such as a wide range of targeted molecules (inorganics, organics, cells, viruses, and bacteria, among others), facile preparation on a massive scale, low molecular weights, high temperature and pH stability, easier modification, and long-term storage stability.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Aptamer Sensors

Shaban

Kim

2021

Sensors

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Recently, aptamers have attracted attention in the biosensing field as signal recognition elements because of their high binding affinity toward specific targets such as proteins, cells, small molecules, and even metal ions, antibodies for which are difficult to obtain. Aptamers are single oligonucleotides generated by in vitro selection mechanisms via the systematic evolution of ligand exponential enrichment (SELEX) process. In addition to their high binding affinity, aptamers can be easily functionalized and engineered, providing several signaling modes such as colorimetric, fluorometric, and electrochemical, in what are known as aptasensors. In this review, recent advances in aptasensors as powerful biosensor probes that could be used in different fields, including environmental monitoring, clinical diagnosis, and drug monitoring, are described. Advances in aptamer-based colorimetric, fluorometric, and electrochemical aptasensing with their advantages and disadvantages are summarized and critically discussed. Additionally, future prospects are pointed out to facilitate the development of aptasensor technology for different targets.

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A dual-function oligonucleotide-based ratiometric fluorescence sensor for ATP detection

Cited by 25 publications

References 48 publications

An electrochemiluminescence resonance energy transfer biosensor for the detection of circulating tumor DNA from blood plasma

An electrochemiluminescence resonance energy transfer biosensor for the detection of circulating tumor DNA from blood plasma

Ratiometric Fluorescence Detection of Colorectal Cancer-Associated Exosomal miR-92a-3p with DSN-Assisted Signal Amplification by a MWCNTs@Au NCs Nanoplatform

Recent Advances in Aptamer Sensors

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