A dual-enzyme, micro-band array biosensor based on the electrodeposition of carbon nanotubes embedded in chitosan and nanostructured Au-foams on microfabricated gold band electrodes

Abstract

We report the development of a dual-enzyme electrochemical biosensor based on microfabricated gold band array electrodes which were first modified by gold foam (Au-foam) in order to dramatically increase the active surface area.

Recent Advancement in Biofluid-Based Glucose Sensors Using Invasive, Minimally Invasive, and Non-Invasive Technologies: A Review

“…The shelf life of these sensors was reported to be 45 days. Chronoamperometry was used to detect glucose in the linear range of 0.05 mM to 1.1 mM with an LOD of 0.025 mM [ 157 ].…”

A Critical Review of Electrochemical Glucose Sensing: Evolution of Biosensor Platforms Based on Advanced Nanosystems

“…However, as mentioned earlier, part of the challenge with these systems arises from the molecular structure of the enzyme itself. Many attempts have been made to try to create a successful matrix for the transfer of electrons between the FAD and the electrode by integration of conductive polymers and emerging nanomaterials such as carbon-based nanomaterials (carbon nanotubes [10][11][12][13][14], graphene [15][16][17], carbon/graphene quantum dots [18][19][20], etc. ), metal nanoparticles [21][22][23][24][25] (gold, silver, copper, etc.…”

“…The electrodeposition of chitosan films is one of the common methods now used in biosensor development [ 77 , 78 , 79 ]. For example, very recently, Juska et al [ 14 ] have reported a biosensor towards glucose based on a two-step electrodeposition process, Figure 6 c. Firstly, these workers achieved the deposition of gold foam on to a microfabricated gold electrode array in acidic environment at high negative applied voltage. Then, an organic functional layer was electrodeposited on to the gold foam surface which consisted of chitosan and multiwalled carbon nanotubes.…”

“…( b ) Schematic displaying the surface modification of Pd@Pt NCs using chitosan solution (CS) biopolymer and the covalent immobilization of GOx to the CS by reacting with GA to cross-link the amino group of CS and the FAD site of GOx [ 30 ]. ( c ) SEM images showing the Au-foam band array after CS-multi-walled carbon nanotubes (MWCNT) electrodeposition (i), the Au-foam/CS-MWCNT pores (ii) and SEM images of the CS-MWCNT nanocomposite film electrodeposited nanostructures with higher magnification (iii,iv), and construction of the Au-foam/CS-MWCNT/HRP-GOx microbiosensor (v) [ 14 ].…”

A Novel Amperometric Glucose Biosensor Based on Fe3O4‐Chitosan‐β‐Cyclodextrin/MWCNTs Nanobiocomposite

“…Multi-walled carbon nanotubes (MWCNTs) is one of the most widely used carbon materials but their applications are normally limited due to their hydrophobic nature. MWCNTs can be functionalized with biomolecules and metal oxide nanoparticles, surfactants and polymers to reinforce the surface properties for challenging the limits [19]. Therefore, a novel electrochemical biosensor based on Fe 3 O 4 À CSÀ CD/MWCNTs/GOx was fabricated for eletrodetection of glucose in this work.…”