A dsRNA virus with filamentous viral particles

Jia, Hengxia; Dong, Kaili; Zhou, Lingling; Wang, Guoping; Hóng, Ní; Jiāng, Dàohóng; Xú, Wénxìng

doi:10.1038/s41467-017-00237-9

Cited by 77 publications

(114 citation statements)

References 28 publications

(63 reference statements)

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“…DsRNA viruses have a wide host range including animals, plants, protozoa, and fungi [42]. Mycoviruses with dsRNA genomes are mostly encapsidated in isometric particles [43] and are currently classified into eight families: Amalgaviridae (1 genomic segment, 3.5 kbp in length), Chrysoviridae (3-7 genomic segments, 2.4-3.6 kbp in length), Megabirnaviridae (2 genomic segments, 7.0-9.0 kbp in length), Partitiviridae (2-3 genomic segments, 1.4-2.3 kbp in length), Quadriviridae (4 genomic segments, 3.7-4.9 kbp in length), Reoviridae (Spinareovirinae subfamily, 10-12 genomic segments, 0.7-5.0 kbp in length) and Totiviridae (non-segmented, 4.6-7 kbp in length) [42,[44][45][46]. Moreover, a dsRNA virus named Botrytis porri RNA virus 1 (BpRV1) belonging to the genus Botybirnavirus has been described [47].…”

Section: Double-stranded Rna Virusesmentioning

confidence: 99%

“…Mycovirus taxonomy regularly changes with the discovery of novel viruses [48] and additional families, such as Alternaviridae (4 genomic segments 1.4-3.6 kbp in length), have been proposed recently. Generally, dsRNA viruses form spherical and not filamentous virions, the latter being a common characteristic of several (+)ssRNA plant and fungal viruses including Alphaflexiviridae, Betaflexiviridae, Gammaflexiviridae, Potyviridae, and Closteroviridae; nevertheless, a novel dsRNA virus from Colletotrichum camelliae isolated from tea plants in China was found to form flexuous and elongated virions [42,44]. Some dsRNA viruses form no true virions but are associated with and coated by viral proteins, as reported recently for Aspergillus fumigatus tetramycovirus-1 (AfuTmV-1) and Beauveria bassiana polymycovirus-1 (BbPmV-1), from the human pathogen A. fumigatus and the insect pathogen B. bassiana, respectively [49,50].…”

Section: Double-stranded Rna Virusesmentioning

confidence: 99%

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Viruses Infecting the Plant Pathogenic Fungus Rhizoctonia solani

Abdoulaye

Foda

Kotta‐Loizou

2019

Viruses

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The cosmopolitan fungus Rhizoctonia solani has a wide host range and is the causal agent of numerous crop diseases, leading to significant economic losses. To date, no cultivars showing complete resistance to R. solani have been identified and it is imperative to develop a strategy to control the spread of the disease. Fungal viruses, or mycoviruses, are widespread in all major groups of fungi and next-generation sequencing (NGS) is currently the most efficient approach for their identification. An increasing number of novel mycoviruses are being reported, including double-stranded (ds) RNA, circular single-stranded (ss) DNA, negative sense (−)ssRNA, and positive sense (+)ssRNA viruses. The majority of mycovirus infections are cryptic with no obvious symptoms on the hosts; however, some mycoviruses may alter fungal host pathogenicity resulting in hypervirulence or hypovirulence and are therefore potential biological control agents that could be used to combat fungal diseases. R. solani harbors a range of dsRNA and ssRNA viruses, either belonging to established families, such as Endornaviridae, Tymoviridae, Partitiviridae, and Narnaviridae, or unclassified, and some of them have been associated with hypervirulence or hypovirulence. Here we discuss in depth the molecular features of known viruses infecting R. solani and their potential as biological control agents.

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Section: Double-stranded Rna Virusesmentioning

confidence: 99%

Section: Double-stranded Rna Virusesmentioning

confidence: 99%

Viruses Infecting the Plant Pathogenic Fungus Rhizoctonia solani

Abdoulaye

Foda

Kotta‐Loizou

2019

Viruses

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“…Rosellinia necatrix megabirnavirus 1 dari jamur busuk akar putih Rosellinia necatrix juga menunjukkan potensi signifikan sebagai kontrol biologis (Yu et al 2013). CcFV-1 (Colletotrichum camelliae filamentous virus 1) merupakan mikovirus yang ditemukan pada Colletotrichum camelliae yang dapat merusak homeostasis seluler inang, menyebabkan hipovirulensi, dan mempengaruhi morfologi inang seperti produksi dan distribusi pigmen, laju pertumbuhan, dan pembentukan cincin hifa (Jia et al 2017).…”

Section: Pendahuluanunclassified

“…Pola pita RNA spesifik selain rRNA dan tRNA juga telah ditemukan pada Fusarium strain C15 dengan ukuran sekitar 1 kbp dan 0.8 kbp yang mengindikasikan keberadaan mikovirus (Supyani dan Widadi 2015). Genom mikovirus pada C. gloeosporioides dilaporkan memiliki ukuran pita dsRNA 2.63-3.397 kbp (Zhong et al 2016) dan 0.99-2.444 kbp dsRNA pada C. camelliae (Jia et al 2017).…”

Section: Pendahuluanunclassified

Keberadaan Mikovirus Berdasarkan Deteksi Berbasis RNA pada Colletotrichum Hipovirulen

et al. 2019

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Colletotrichum is one of phytopathogenic fungi causing large economic losses in more than 3000 plant species in the world. Colletotrichum causes anthracnose disease with sunken and concentric ring-shaped of necrosis symptoms on leaves and fruits. Chilli production has reported to have yield losses 10%-80% and production of Acacia spp. experiencing losses at the nursery stage due to anthracnose. Mycovirus is a biological control agent that has potential to develop through its hypovirulence effect generated on host pathogenic fungi. Mycovirus infect host fungi and replicate rapidly in host cells also can be transmitted through horizontal and vertical transmission. This research was purposed to detect the existence of mycoviruses in hypovirulent Colletotrichum. Stages of this research comprised Colletotrichum isolation, colony morphological observation, virulence assay on apples fruit, and RNA analysis included RNA extraction based on PrestoTM Mini RNA Yeast Kit protocol and RNA visualization on agarose gel using UV transilluminator. The results of RNA visualization showed the existence of specific RNA bands outside rRNA and tRNA bands in D3 and D9 isolates which were considered as RNA bands of mycovirus. The specific RNA bands in D3 isolate sized around 2 kbp and 3 kbp while D9 isolate showed specific RNA bands sized around 0.6 kbp and 3 kbp.

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“…This is not the case for AfuPmV-1 but an uncharacterized virus with an identical electrophoretic profile isolated from A. fumigatus isolate A78 may harbor a fifth dsRNA segment (Readman, 2011 ; Figure 4B ). Interestingly, a recently published related mycovirus from the plant pathogenic fungus Colletotrichum camelliae has four additional dsRNA segments, is also infectious as dsRNA and forms filamentous virions (Jia et al, 2017 ).…”

Section: Molecular Characterisation Of the Aspergillus mentioning

confidence: 99%

Mycoviruses in Aspergilli: A Comprehensive Review

2017

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Fungi, similar to all species, are susceptible to viral infection. Aspergillus is arguably the most well studied fungal genus because of its medical, ecological and economical significance. Mycoviruses were initially detected in Aspergillus species almost 50 years ago and the field continues to be active today with ground-breaking discoveries. The aim of the present review is to cover the scientific progress in all aspects of mycovirology as exemplified by Aspergillus-focused research. Initially an overview of the population studies illustrating the presence of mycoviruses in numerous important Aspergillus species, such as A. niger, A. flavus, and A. fumigatus with be presented. Moreover the intricacies of mycovirus transmission, both inter- and intra-species, will be discussed together with the methodologies used to investigate viral dispersion in a laboratory setting. Subsequently, the genomic features of all molecularly characterized mycoviruses to date will be analyzed in depth. These include members of established viral families, such as Partitiviridae, Chrysoviridae and Totiviridae, but also more recent, novel discoveries that led to the proposal of new viral families, such as Polymycoviridae, Alternaviridae and, in the context of the present review, Exartaviridae. Finally, the major issue of phenotypic effects of mycoviral infection on the host is addressed, including aflatoxin production in A. flavus, together with growth and virulence in A. fumigatus. Although the molecular mechanisms behind these phenomena are yet to be elucidated, recent studies suggest that by implication, RNA silencing may be involved.

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A dsRNA virus with filamentous viral particles

Cited by 77 publications

References 28 publications

Viruses Infecting the Plant Pathogenic Fungus Rhizoctonia solani

Viruses Infecting the Plant Pathogenic Fungus Rhizoctonia solani

Keberadaan Mikovirus Berdasarkan Deteksi Berbasis RNA pada Colletotrichum Hipovirulen

Mycoviruses in Aspergilli: A Comprehensive Review

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