A Drosophila Homolog of Cadherin Associated with Armadillo and Essential for Embryonic Cell-Cell Adhesion

Apical and lateral cell protrusions interconnect epithelial cells in live Drosophila wing imaginal discs

“…Stained tissues were observed with a ZEISS Laser Scan-ning Microscope 510 Meta (Carl Zeiss AG, Jena). Primary antibodies used in this study were mouse anti-Fasciclin III 7G10 (1:100, Developmental Studies Hybridoma Bank), rabbit anti-GFP (1:2,000, Clontech 8372-1), and rat anti-DE-Cad (DCAD1 and DCAD2, 1:100; (Oda et al, 1994). Secondary antibodies used were Alexa-488-, Alexa-594-(Molecular Probes, Eugene, OR), and Cy5-(Jackson ImmunoResearch, West Grove, PA) conjugated anti-rabbit, anti-mouse, or anti-rat IgG (1:200).…”

Wingless signaling modulates cadherin-mediated cell adhesion inDrosophilaimaginal disc cells

“…Western immunoblot analysis was performed as described previously (Van Leeuwen et al, 1994). The following primary antibodies were used: rabbit polyclonal anti-mouse E-cadherin (Marrs et al, 1993) diluted 1:10,000; mouse monoclonal anti-Arm N27A1 (Peifer, 1993) diluted 1:1,000; rat monoclonal anti-␣-catenin DCAT-1 (Oda et al, 1993) diluted 1:1,000; rat monoclonal anti-DE-cadherin DCAD-2 (Oda et al, 1994) diluted 1:50; rat polyclonal anti-Dsh region 1 (Yanagawa et al, 1995) diluted 1:1,000. Quantification of western blots treated with ECL reagent (Amersham) was performed by densitometric scanning of exposed films.…”

“…Imaginal discs were fixed in 4% formaldehyde and stained according to standard procedures. The following antibodies were used: rat monoclonal anti-mouse E-cadherin DECMA-1 (Vestweber and Kemler, 1985) diluted 1:20; mouse monoclonal anti-Arm N27A1 (Peifer, 1993) diluted 1:4; rat monoclonal anti-␣-catenin DCAT-1 (Oda et al, 1993), diluted 1:50; rabbit polyclonal anti-Wg ( Van den Heuvel et al, 1989), affinity purified, diluted 1:20; rat monoclonal anti-DE-cadherin DCAD-2 (Oda et al, 1994), diluted 1:20. After several washes in TBSC, cells were incubated with secondary antibodies in TBSC/5% normal donkey serum.…”

“…Electrophoresis, transfer of RNA to nylon filters and radioactive labeling of DNA was performed according to standard procedures. A 415 bp XbaI-EcoRI fragment from the C terminus of the Arm cDNA (Riggleman et al, 1989) was used as a hybridization probe for Arm, and a 4 kb XbaI-BglII fragment of the DE-cadherin cDNA (Oda et al, 1994) was used as a probe for DEcadherin.…”

Phenotypic analysis of null mutants for DE‐cadherin and Armadillo in Drosophila ovaries reveals distinct aspects of their functions in cell adhesion and cytoskeletal organization

“…After rinsing in PBS, the samples were permeabilized with 0.1% Triton X-100 for 30 min on ice, blocked with 1% BSA in PBS (PBS-BSA), and incubated with primary antibodies at an appropriate dilution in PBS-BSA overnight at 4 ЊC. We used a mixture of mAbs, DCAD1 and DCAD2 for DE-cadherin staining (Oda et al 1994a), mAb N2-7A1 for Armadillo (Peifer 1993), and mAb DCAT-1 for Da-catenin (Oda et al 1993), which were diluted 1 : 20, 1 : 200 and 1 : 20, respectively. After a wash in PBS for 30 min, the ovaries were incubated with secondary antibodies conjugated with biotin (Amersham, UK) for 2 h at room temperature, and then, after another 30-min wash, the ovaries were incubated with streptavidin-FITC conjugate (Amersham) for 90 min at room temperature.…”