A droplet-based piezoelectric concave diaphragm biosensor with self-enhancing functionality for label-free detection of protein–ligand interactions

Feng, Guo-Hua; Lin, Zhi-Dian

doi:10.1016/j.snb.2013.03.087

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…In order to increase the sensitivity, a great deal of research effort has been focused on the development of AW sensors with a smaller size/mass, such as MEMS/NEMS (micro/nano‐electro‐mechanical system). For example, a smaller QCM (Kao et al, ), micro/nano‐cantilevers (Ilic et al, ), micro‐diaphragm (Feng and Li, ; Lu et al, ; Yang et al, ), and other micro‐resonators have been investigated as transducers used in the development of high performance biosensors.…”

Section: Introductionmentioning

confidence: 99%

Magnetostrictive particle based biosensors for in situ and real‐time detection of pathogens in water

Zhang

et al. 2014

Biotech & Bioengineering

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Biosensors for in situ detection of pathogenic bacteria in liquid are developed using magnetostrictive particles (MSP) as the sensor platform. The sensing elements used are phage E2 against Salmonella typhimurium, monoclonal antibody against Listeria monocytogenes, polyclonal antibody against Escherichia coli, and polyclonal antibody against Staphylococcus aureus, respectively. These biosensors were characterized in cultures with different populations ranging from 5 × 10(1) to 5 × 10(8) cfu/mL. It is found that the MSP-based biosensors work well in water and have a rapid response with a response time in minutes, which makes the MSP-based sensors suitable for in situ and real-time detection of pathogenic bacteria in liquid. The experimental results show that all MSP-phage and MSP-antibody biosensors in size of 1.0 mm × 0.3 mm × 15 µm exhibit a detection limit better than 100 cfu/mL. Based on the Hill plot, it is concluded that each bacterial cell is bound onto the sensor surface through about four-to-five sites. When the cultures with low population (<10(6) cfu/mL) are tested, both MSP-phage and MSP-antibody sensors exhibit the similar response. However, the phage-MSP sensors exhibit a higher capability in the capture of target bacterial cell.

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Section: Introductionmentioning

confidence: 99%

Magnetostrictive particle based biosensors for in situ and real‐time detection of pathogens in water

Zhang

et al. 2014

Biotech & Bioengineering

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“…Piezoelectric-actuated resonating sensors are a promising and widely used type of sensors for detecting biomolecules or viscous media 1 2 3 4 5 6 . Each sensor has its own resonant frequency 7 .…”

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confidence: 99%

Enhancing the sensitivity of a micro-diaphragm resonating sensor by effectively positioning the mass on the membrane

Kim

Cho

et al. 2015

Sci Rep

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The detection of biomarkers in the liquid phase using mechanical sensors is difficult because of noise caused by the liquid. To reduce and verify the side effects of liquid loading, we performed calculations and experiments to determine the shift in resonant frequency according to the loading conditions. A 2-μm-thick piezoelectric rectangular micro-diaphragm with a 500 × 500 μm membrane was used. These dimensions were determined such that there would be an analogous resonant frequency shift ratio in both (1, 1) and (2, 2) modes. By calculating and measuring the resonant frequency, we verified that the resonant frequency of the sensor would change only through contact with the liquid, even the resonant frequency change by only liquid much higher than the changes caused by the nanoparticles. The real signal constituted only 0.017% of the initial resonant frequency. To enhance the sensitivity by reducing the unexpected surface stress in the liquid, the liquid was dropped onto the surface of the micro-diaphragm. This resulted in an improvement of more than 10 times the sensitivity in both modes. In addition, by controlling the position in the micro-diaphragm resonating sensor, more sensitive positions with large displacements were determined according to each mode.

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A droplet-based piezoelectric concave diaphragm biosensor with self-enhancing functionality for label-free detection of protein–ligand interactions

Cited by 2 publications

References 20 publications

Magnetostrictive particle based biosensors for in situ and real‐time detection of pathogens in water

Magnetostrictive particle based biosensors for in situ and real‐time detection of pathogens in water

Enhancing the sensitivity of a micro-diaphragm resonating sensor by effectively positioning the mass on the membrane

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