A Double Staining Method for Histone H3 mRNA and p53 Protein in Oral Tumors Using In Situ Hybridization and Immunohistochemistry.

Nishikawa, Tetsunari; Arai, Shoichi; Uobe, Kenichi; Wato, Masahiro; Tominaga, Kazuhiro; Masuno, Kazuya; Mori, Masahiko; Yoshida, Seiko; Kobayashi, Hideki; Tanaka, Akio

doi:10.1267/ahc.32.327

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…These sections were visualized with 3-amino-9ethylcarborate-HCl (AEC, red color, Dako) for 10 min to detect the p53 protein, and with 5-bromo-4-chloro-3-indoxyl phosphate/nitro blue tetrazolium (BCIP/NBT, blue violet color, Dako) for 90 min to detect histone H3 mRNA. These sections were rinsed with distilled water and mounted in glycerol water mounting medium (14).…”

Section: Double Staining For Histone H3 Mrna and P53 Proteinmentioning

confidence: 99%

Expression of histone H3, p53, cyclin D1, and cyclin B1 in oral mucosal lesions

2009

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The purpose of the present study was to clarify kinetic aberrations and premalignant and malignant lesions of oral mucosa. Epithelial hyperplasia (EH), mild and moderate epithelial dysplasia (ED), carcinoma in situ (CIS), and squamous cell carcinoma (SCC) were evaluated for histone H3 mRNA, p53 protein (P53), cyclin D1, and cyclin B1. A few cases were positive for P53 and histone H3, but rarely coexpressed both in EH and ED. In CIS and SCC, increased expressions of P53 and histone H3 were seen, and there were also many cases showing extensive co-expression of both. There were many cyclin D1-positive cases of EH and ED. In CIS and SCC, increased numbers of cyclin D1-positive and cyclin B1-positive cells were apparent, and these cells were distributed widely. In cases of CIS with co-expression for histone H3 and P53, there was increased and wide distribution of cyclin D1-and cyclin B1-positive cells. These features of CIS may indicate that cell proliferation is induced, and that zonal differentiation of stratified squamous epithelium is irregular as seen in SCC.

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Section: Double Staining For Histone H3 Mrna and P53 Proteinmentioning

confidence: 99%

Expression of histone H3, p53, cyclin D1, and cyclin B1 in oral mucosal lesions

2009

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“…The procedure of the double staining for histone H3 mRNA and p53 protein has been reported earlier [19]. The deparaffinized sections were first hybridized with an FITClabeled single-strand DNA probe for human histone H3 mRNA (histone H3 probe, Dako, USA), and incubated with alkaline phosphatase-conjugated anti-FITC rabbit antibody (Dako, Denmark).…”

Section: Double Staining For Histone H3 Mrna and P53 Proteinmentioning

confidence: 99%

“…Histone H3 mRNA is thus used to detect cell proliferation by in situ hybridization and utilized as a marker of proliferating cells [10]. The double staining both in in situ hybridization for histone H3 mRNA and immunohistochemical staining for p53 protein has previously been described as an effective method for simultaneous identification of the proliferative and aberrant cells in tumor tissues [19].…”

Section: Introductionmentioning

confidence: 99%

Aberrant Expression Patterns of Histone H3 mRNA, p53, Cyclin D1, and Cyclin B1 in Oral Neoplastic Epithelial Lesions

Nishikawa

Hara

Itô

et al. 2005

Acta Histochem. Cytochem

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In this paper, tumor growth was studied using oncogenic and kinetic analysis. In verrucous carcinoma (VC, n=22), squamous papilloma (SP, n=18), and hyperkeratotic lesion (HK, n=12), cellular proliferative potentials were evaluated and compared. Co-expression of histone H3 mRNA and p53 protein was not found in HK. In 6 cases (33.3%) of SP, co-expression was detected within the basal and parabasal layers. However, in 17 cases (77.2%) of VC, co-expression of histone H3 mRNA and p53 protein was found extensively from basal to spinous layers. Cyclin D1 was detected in 5 cases (41.7%) of HK and in 14 cases (77.8%) of SP at parabasal layer. While in 20 cases (90.9%) of VC, cyclin D1 was detected from basal to spinous layers, and positive (2+ and 3+) cases (45.4%) increased.Cyclin B1 was detected in 9 cases (75.0%) of HK and in 16 cases (88.9%) of SP at basal or parabasal layer, and in all 22 cases of VC from basal to spinous layers, and positive (2+ and 3+) cases (68.1%) increased. In the intra-cellular distribution of cyclin B1, there were many cases of cytoplasmic types (66.7%) in HK, of cytoplasmic dominant types (50.0%) in SP, and of cytoplasmic dominant types (72.7%) in VC. There were also some cases of nuclear dominant types (27.3%) in VC. The present study suggests that increase of p53 protein, increase of cell proliferation, wide epithelial distribution of cyclins D1 and B1, and increase of positive cells in the nucleus with cyclin B1 were indicators of malignant potential.

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