A dot enzyme linked immunosorbent assay (dot ELISA): Comparison with standard fluorescent antibody test (FAT) for the diagnosis of rabies in animals

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

“…Positive reactions were easily visualized as red dots after enzyme degradation of the substrate. Dot-ELISA test for rabies detection was found to be specific, faster and reliable with almost 92% sensitive which is similar to the earlier reports [18,24]. Although, Dot ELISA test did not produce non-specific false-positive results [19], development of colour spot depends on the concentration of virus in antigen used in the test.…”

“…The experimental protocol for Dot-ELISA was adapted from Jayakumar et al [18]. In brief 1 ll of brain suspension was dotted on to 0.2 lm nitrocellulose paper and dried at room temperature for 30 min.…”

Rapid diagnosis of rabies in humans and animals by a dot blot enzyme immunoassay

“…The assay itself is a simple procedure consisting of the application of the brain homogenate (antigen) as a dot on the PVDF membrane, followed by drying and subsequent detection using biotinylated antinucleoprotein antibody and color development with DAB. The earlier work carried out by Jayakumar et al used similar principles but they used antiserum to the whole virus and nitrocellulose membrane for blotting 14 .…”

Characterization and potential diagnostic application of monoclonal antibodies specific to rabies virus

“…ELISA is economical, consumes fewer reagents and fits well with current facilities in many underdeveloped and developing countries where diagnostic methods are less sophisticated. A very small quantity of suspect brain material may be obtained by using a drinking straw or plastic pipette through the occipital or retroorbital route as recommended earlier[28]. This reduces any danger to the laboratory worker who conducts brain removal; in addition, materials can be transported in Eppendorf tubes containing 0.5 mL of formalin on ice.…”