Rapid diagnosis of rabies in humans and animals by a dot blot enzyme immunoassay

Madhusudana, Shampur Narayan; Paul, Joel; Abhilash, Venugopal Karavattu; Suja, M. S.

doi:10.1016/j.ijid.2004.02.006

Cited by 30 publications

(13 citation statements)

References 8 publications

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“…Positive reactions were easily visualized as red dots after enzyme degradation of the substrate. Dot-ELISA test for rabies detection was found to be specific, faster and reliable with almost 92% sensitive which is similar to the earlier reports [18,24]. Although, Dot ELISA test did not produce non-specific false-positive results [19], development of colour spot depends on the concentration of virus in antigen used in the test.…”

supporting

confidence: 83%

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Kashte

Sherikar

Pingale³

2011

Indian J. Virol.

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Present study was undertaken to compare various routine laboratory diagnostic tests for rabies detection. Seller's staining, mouse inoculation test (MIT), Dot-ELISA, Agar gel precipitation test (AGPT) and counter immunoelectrophoresis test (CIET) were the main basic tests performed in the laboratory for the rabies diagnosis. Out of 200 brain specimens, Negri bodies were observed in 52 brain samples by Seller's staining. Rabies virus was isolated in 56 samples by intra-cerebral inoculation in newborn Swiss-albino mice. Dot-ELISA and AGPT could detect rabies antigen in 55 and 57 samples respectively. Comparative analysis revealed that the CIET is the most sensitive and rapid test among performed diagnostic tests.

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supporting

confidence: 83%

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Kashte

Sherikar

Pingale³

2011

Indian J. Virol.

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“…The bacterial suspension was blotted directly onto nitrocellulose membranes using a 96-multiwell microfiltration blotter (BioRad Laboratories, Hercules, Calif., U.S.A.) (1,6,12,14). After blocking with 10% skimmilk in PBS for 30 min, the membranes were exposed to 1 mg/ml of PlyGB-GST for 60 min and then 200 ng/ml horseradish peroxidase (HRP)-conjugated mouse anti-GST mAb (GST (B-14) HRP; Santa Cruz, Calif., U.S.A.) for 60 min.…”

Section: Methodsmentioning

confidence: 99%

Sensitive Detection of Bacillus anthracis Using a Binding Protein Originating from γ‐Phage

Fujinami

Hirai

Sakai

et al. 2007

Microbiology and Immunology

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Detection of biological weapons is a primary concern in force protection, treaty verification, and safeguarding civilian populations against domestic terrorism. One great concern is the detection of Bacillus anthracis, the causative agent of anthrax. Therefore, there is a pressing need to develop novel methods for rapid, simple, and precise detection of B. anthracis. Here, we report that the C‐terminal region of γ‐phage lysin protein (PlyG) binds specifically to the cell wall of B. anthracis and the recombinant protein corresponding to this region (positions, 156–233), PlyGB, is available as a bioprobe for detection of B. anthracis. Our detection method, based on a membrane direct blot assay using recombinant PlyGB, was more rapid and sensitive than the γ‐phage test and was simpler and more inexpensive than genetic methods such as PCR, or immunological methods using specific antibodies. Furthermore, its specificity was comparable to the γ‐phage test. PlyGB is applicable in conventional methods instead of antibodies and could be a potent tool for detection of B. anthracis.

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“…Other tests for rabies viral antigen detection include a dot-blot immunoassay for brain tissues [53] and an enzyme immunoassay for rapid diagnosis of rabies in humans and animals [54]. …”

Section: Newer Diagnostic Tests For Rabiesmentioning

confidence: 99%

Laboratory Diagnosis of Human Rabies: Recent Advances

Mani

Madhusudana

2013

The Scientific World Journal

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Rabies, an acute progressive, fatal encephalomyelitis, transmitted most commonly through the bite of a rabid animal, is responsible for an estimated 61,000 human deaths worldwide. The true disease burden and public health impact due to rabies remain underestimated due to lack of sensitive laboratory diagnostic methods. Rapid diagnosis of rabies can help initiate prompt infection control and public health measures, obviate the need for unnecessary treatment/medical tests, and assist in timely administration of pre- or postexposure prophylactic vaccination to family members and medical staff. Antemortem diagnosis of human rabies provides an impetus for clinicians to attempt experimental therapeutic approaches in some patients, especially after the reported survival of a few cases of human rabies. Traditional methods for antemortem and postmortem rabies diagnosis have several limitations. Recent advances in technology have led to the improvement or development of several diagnostic assays which include methods for rabies viral antigen and antibody detection and assays for viral nucleic acid detection and identification of specific biomarkers. These assays which complement traditional methods have the potential to revolutionize rabies diagnosis in future.

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Rapid diagnosis of rabies in humans and animals by a dot blot enzyme immunoassay

Abstract: The dot blot enzyme immunoassay (DIA) test described here is a sensitive, specific and rapid test for the post-mortem diagnosis of rabies in animals and humans. The utility of this test for the ante-mortem diagnosis of rabies needs to be further evaluated.

Cited by 30 publications

References 8 publications

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Comparative Analysis of Routine Laboratory Diagnostic Tests for Rabies

Sensitive Detection of Bacillus anthracis Using a Binding Protein Originating from γ‐Phage

Laboratory Diagnosis of Human Rabies: Recent Advances

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