A Dose Threshold for a Medium Transfer Bystander Effect for a Human Skin Cell Line

Liu, Zhengfeng; Mothersill, Carmel; McNeill, Fiona E.; Lyng, Fiona M.; Byun, S.H.; Seymour, Colin; Prestwich, W.V.

doi:10.1667/rr3580.1

Cited by 126 publications

(81 citation statements)

References 20 publications

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“…In this study, significant cell death was observed in cells that were treated with 0.005, 0.05 and 0.5 Gy ICCM and this is similar to our previous studies (42,44,45). Increased viability was observed in cells that were treated with 0.05 and 0.5 Gy ICCM from which exosomes were removed compared to 0 Gy ICCM-Exo treated cells.…”

Section: Discussionsupporting

confidence: 90%

Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

Jella¹,

et al. 2014

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Section: Discussionsupporting

confidence: 90%

Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

Jella¹,

et al. 2014

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“…These differences may be due to differences in experimental design as in the present study ICCM was transferred to unirradiated cells 1 hour after irradiation, while in the study of Harada et al (2008), irradiated cells were co-cultured with unirradiated cells immediately after irradiation. Liu et al 2006). Reductions of approx 20-30% in clonogenic survival have also been reported previously (Liu et al 2006) for both direct and bystander treatments at the higher doses used in the present study, 0.05Gy and 0.5Gy.…”

Section: Discussionsupporting

confidence: 87%

“…Liu et al 2006). Reductions of approx 20-30% in clonogenic survival have also been reported previously (Liu et al 2006) for both direct and bystander treatments at the higher doses used in the present study, 0.05Gy and 0.5Gy. The human keratinocyte HPV-G cell line shows a similar bystander response to the HaCaT cell line used in the present study.…”

Section: Discussionsupporting

confidence: 87%

“…The human keratinocyte HPV-G cell line shows a similar bystander response to the HaCaT cell line used in the present study. In our previous work (Liu et al 2006), a dose threshold for bystander effects was reported with a reduction in clonogenic survival and calcium fluxes observed above 3mGy. In the present study, cell viability was measured 24…”

Section: Discussionmentioning

confidence: 86%

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Reactive oxygen species-induced release of signalling factors in irradiated cells triggers membrane signalling and calcium influx in bystander cells

Lyng¹,

Howe²,

McClean

2011

International Journal of Radiation Biology

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“…57 The proposal of a dose threshold for triggering a bystander effect agreed with a previous study on γ-ray-induced bystander effect. 58 ■ ASSOCIATED CONTENT…”

Section: Environmental Science and Technologymentioning

confidence: 99%

Bystander Effect between Zebrafish Embryos in Vivo Induced by High-Dose X-rays

Choi

Kobayashi³

et al. 2013

Environ. Sci. Technol.

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ABSTRACT:We employed embryos of the zebrafish, Danio rerio, for our studies on the in vivo bystander effect between embryos irradiated with highdose X-rays and naive unirradiated embryos. The effects on the naive whole embryos were studied through quantification of apoptotic signals at 25 h post fertilization (hpf) through the terminal dUTP transferase-mediated nick endlabeling (TUNEL) assay followed by counting the stained cells under a microscope. We report data showing that embryos at 5 hpf subjected to a 4-Gy X-ray irradiation could release a stress signal into the medium, which could induce a bystander effect in partnered naive embryos sharing the same medium. We further demonstrated that this bystander effect (induced through partnering) could be successfully suppressed through the addition of the nitric oxide (NO) scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) into the medium but not through the addition of the CO liberator tricarbonylchloro(glycinato)ruthenium(II) (CORM-3). This shows that NO was involved in the bystander response between zebrafish embryos induced through X-ray irradiation. We also report data showing that the bystander effect could be successfully induced in naive embryos by introducing them into the irradiated embryo conditioned medium (IECM) alone, i.e., without partnering with the irradiated embryos. The IECM was harvested from the medium that had conditioned the zebrafish embryos irradiated at 5 hpf with 4-Gy X-ray until the irradiated embryos developed into 29 hpf. NO released from the irradiated embryos was unlikely to be involved in the bystander effect induced through the IECM because of the short life of NO. We further revealed that this bystander effect (induced through IECM) was rapidly abolished through diluting the IECM by a factor of 2× or greater, which agreed with the proposal that the bystander effect was an on/off response with a threshold.

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A Dose Threshold for a Medium Transfer Bystander Effect for a Human Skin Cell Line

Cited by 126 publications

References 20 publications

Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

Reactive oxygen species-induced release of signalling factors in irradiated cells triggers membrane signalling and calcium influx in bystander cells

Bystander Effect between Zebrafish Embryos in Vivo Induced by High-Dose X-rays

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