Reactive oxygen species-induced release of signalling factors in irradiated cells triggers membrane signalling and calcium influx in bystander cells

Lyng, Fiona M.; Howe, Orla; McClean, Brendan

doi:10.3109/09553002.2010.549533

Cited by 65 publications

(44 citation statements)

References 49 publications

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“…Previous work from our laboratory has shown that bystander signals released into ICCM can trigger membrane signaling and an influx of calcium followed by induction of ROS in unirradiated cells (41). The present study has shown that these bystander signals may be communicated through the secretion of exosomes or microvesicles.…”

supporting

confidence: 51%

“…There have been many studies on membrane signaling in bystander cells (41,(50)(51)(52)(53). Rapid membrane signaling, calcium signaling and ROS induction has been reported previously in unirradiated cells after the addition of ICCM (41).…”

Section: Discussionmentioning

confidence: 88%

“…Cell viability was measured using the alamarBlue (AB) assay as described previously for bystander cell death (41). The alamarBlue assay was performed by using alamarBlue solution (Biosource UK, Nivelles, Belgium).…”

Section: Measurement Of Cell Viability Using the Alamarbluet Assaymentioning

confidence: 99%

See 2 more Smart Citations

Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

Jella¹,

et al. 2014

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supporting

confidence: 51%

Section: Discussionmentioning

confidence: 88%

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Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

Jella¹,

et al. 2014

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“…Calcium fluxes have previously been shown to be induced in HPV-G reporter cells exposed to irradiated cell conditioned media (ICCM) (Lyng et al 2011, Shao et al 2006, implicating Ca 2+ signalling in the bystander signalling and/or response. This calcium response has been associated with a subsequent loss of mitochondrial membrane potential and ultimately increased apoptosis rates (Lyng et al 2002.…”

Section: Camentioning

confidence: 99%

The effect of genetic background and dose on non-targeted effects of radiation

Irons¹,

Serra²,

Bowler³

et al. 2012

International Journal of Radiation Biology

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Purpose: This work investigates the hypothesis that genetic background plays a significant role in the signalling mechanisms underlying induction and perpetuation of genomic instability following radiation exposure. Materials and methods:Bone marrow from two strains of mice (CBA and C57), were exposed to a range of X-ray doses (0, 0.01, 0.1, 1 and 3 Gy). Different cellular signalling endpoints: apoptosis, cytokine levels and calcium flux, were evaluated at 2h, 24h and 7d post-irradiation to evaluate immediate and delayed effects.Results: In CBA (radio-sensitive) elevated apoptosis levels were observed at 24h post X-irradiation, transforming growth factor-β (TGF-β) levels were additionally shown to increase with time and dose. C57 showed a higher background level of apoptosis compared to CBA, which was sustained 7 days after radiation exposure. Levels of tumor necrosis factor-α (TNF-α) were also increased at day 7 for higher X-ray doses. TGF-β levels were higher in CBA, whilst C57 exhibited a greater TNF-α response.Calcium flux was induced in reporter cells on exposure to conditioned media from both strains. Conclusions:These results show genetic and dose specific differences in radiation-induced signalling in the initiation and perpetuation of the instability process, which have potential implications on evaluation of non-targeted effects in radiation risk assessment.3

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“…This assay has been shown to be more sensitive than the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay (Hamid et al 2004). The Alamar blue assay has been used previously by our group (Lyng et al 2011) to measure bystander induced cell death and shows comparable results to the clonogenic assay when the assay is performed at 96 hours after exposure.…”

Section: Introductionmentioning

confidence: 99%

The importance of serum serotonin levels in the measurement of radiation-induced bystander cell death in HaCaT cells

Lyng

Desplanques

Jella

et al. 2012

International Journal of Radiation Biology

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Purpose: The aim of this study was to investigate the importance of serum serotonin levels in the measurement of bystander cell death. The study was undertaken as part of an intercomparison exercise involving seven European laboratories funded under the European Union Sixth Framework Programme (FP6) Non-Targeted Effects (NOTE) integrated project. Conclusions: The data suggest that in our cell system and with our endpoints (clonogenic assay and Alamar blue assay), serum serotonin levels do not play a role in bystander induced cell death.

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Reactive oxygen species-induced release of signalling factors in irradiated cells triggers membrane signalling and calcium influx in bystander cells

Cited by 65 publications

References 49 publications

Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

Exosomes Are Involved in Mediating Radiation Induced Bystander Signaling in Human Keratinocyte Cells

The effect of genetic background and dose on non-targeted effects of radiation

The importance of serum serotonin levels in the measurement of radiation-induced bystander cell death in HaCaT cells

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