A Dopamine Acrylamide Molecule for Promoting Collagen Biomimetic Mineralization and Regulating Crystal Growth Direction

Abstract

The reconstruction of the intra/interfibrillar mineralized collagen microstructure is extremely important in biomaterial science and regeneration medicine. However, certain problems, such as low efficiency and long period of mineralization, are apparent, and the mechanism of interfibrillar mineralization is often neglected in the present literature. Thus, we propose a novel model of biomimetic collagen mineralization that uses molecules with the dual function of cross-linking collagen and regulating collagen mineralization to construct the intrafibrillar and interfibrillar collagen mineralization of the structure of mineralized collagen hard tissues. In the present study completed in vitro, N-2-(3,4-dihydroxyphenyl) acrylamide (DAA) is used to bind and cross-link collagen molecules and further stabilize the selfassembled collagen fibers. The DAA−collagen complex provides more affinity with calcium and phosphate ions, which can reduce the calcium phosphate/collagen interfacial energy to promote hydroxyapatite (HA) nucleation and accelerate the rate of collagen fiber mineralization. Besides inducing intrafibrillar mineralization, the DAA−collagen complex mineralization template can realize interfibrillar mineralization with the c-axis of the HA crystal on the surface of collagen fibers and between fibers that are parallel to the long axis of collagen fibers. The DAA−collagen complex, as a new type of mineralization template, may provide a new collagen mineralization strategy to produce a mineralized scaffold material for tissue engineering or develop bone-like materials.

The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica

“…To stabilize the initial environment, the specimens were held at 20 °C for 5 min. They were then heated at 5 K·min −1 to 800 °C under a nitrogen atmosphere (20 mL·min −1 ) [ 28 ].…”

Oxidized Dopamine Acrylamide Primer to Achieve Durable Resin–Dentin Bonding

“…When the direction of the collagen fibers’ long axis was parallel to the longitudinal sections, the HA crystals appeared as a line that was bundled together of intrafibrillar and interfibrillar (Fig. 3 D; yellow double-headed arrows indicate the HA crystals arrangement direction) [ 25 ]. When the direction of the collagen fibers was perpendicular or at a certain angle to the longitudinal sections, the HA crystals appeared as dots that were bundled together by the intrafibrillar and interfibrillar (Fig.…”

“…Our previous research has shown that mussel adhesive protein may inhibit MMP activity and facilitate dentin adhesion [ 23 ], and a DOPA derivative of N-2-(3,4-dihydroxylphenyl) acrylamide (DAA) was designed and synthesized (Fig. S1 ) to effectively bind collagen; improve cross-linking degree, thermal stability, resistance to enzymatic hydrolysis, and mechanical properties of collagen; and also induce collagen intra- and interfibrillar mineralization [ 24 , 25 ]. In addition, DAA has an amide (–CO–NH–) group, which differs from the ester group (–CO–O–R–) in traditional acrylate and methacrylate adhesive monomers.…”

Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels

“…Inspired by the adhesion chemistry of marine mussels, various hydrogels formed by derivatives with catechol groups have wide application prospects in tissue engineering scaffolds [ 21 , 22 ]. Studies indicate that catechol groups have a strong affinity for various organic and/or inorganic surfaces and biomacromolecules that can improve hydrogels’ mechanical properties [ 23 , 24 , 25 ]. As catechol and amino acids derivatives, dopamine (DA) has good adhesion and biocompatibility.…”