Preparation and Properties of Double-Crosslinked Hydroxyapatite Composite Hydrogels

Abstract

Natural polymer hydrogels have good mechanical properties and biocompatibility. This study designed hydroxyapatite-enhanced photo-oxidized double-crosslinked hydrogels. Hyaluronic acid (HA) and gelatin (Gel) were modified with methacrylate anhydride. The catechin group was further introduced into the HA chain inspired by the adhesion chemistry of marine mussels. Hence, the double-crosslinked hydrogel (HG) was formed by the photo-crosslinking of double bonds and the oxidative-crosslinking of catechins. Moreover, hydroxyapatite was introduced into HG to form hydroxyapatite-enhanced hydrogels (HGH). The results indicate that, with an increase in crosslinking network density, the stiffness of hydrogels became higher; these hydrogels have more of a compact pore structure, their anti-degradation property is improved, and swelling property is reduced. The introduction of hydroxyapatite greatly improved the mechanical properties of hydrogels, but there is no change in the stability and crosslinking network structure of hydrogels. These inorganic phase-enhanced hydrogels were expected to be applied to tissue engineering scaffolds.

Biocompatible nanocomposite scaffolds based on carrageenan incorporating hydroxyapatite and hesperidin loaded nanoparticles for bone tissue regeneration

“…This could be due to interaction of HA with polymeric chains, resulting in increased physical crosslinking density and decreasing in pore size of hydrogel, which subsequently reduced the ability of hydrogel to water adsorb 37 . In addition, the dense polymer network made the hydrogel structure more stable and the degradation rate lower 38 …”

“…37 In addition, the dense polymer network made the hydrogel structure more stable and the degradation rate lower. 38…”

Injectable hybrid hydrogels enable enhanced combination chemotherapy and roused anti-tumor immunity in the synergistic treatment of pancreatic ductal adenocarcinoma

“…In general, in situ injection of a solution could transude from the injection site and randomly migrate, leading to limited clinical application, whereas in situ injection of hydrogels mitigates this issue [ 40 ]. Chitosan (CS), a most plentiful natural polysaccharide, has valuable characteristics of biocompatibility and biodegradability [ 41 ]. We utilized chitosan as hydrogels material to cross-link with β-sodium glycerophosphate (β-GP).…”

Stimulus-Responsive, Gelatin-Containing Supramolecular Nanofibers as Switchable 3D Microenvironments for Cells

“…Gel permeation chromatography (GPC) was used to determine the number-average molecular weight ( M n ), weight-average molecular weight ( M w ), and molecular weight distribution ( Ð , M w / M n ) [ 31 , 32 ]. Chromatograms were measured at 25 °C using an EcoSEC ® system (HLC-8320, TOSOH, Tokyo, Japan) equipped with a TSKgel guard column (SuperAW-L, TOSOH) and a refractive index (RI) detector.…”