A dominant negative allele of the Drosophila leucine zipper protein Bunched blocks bunched function during tissue patterning

Ash, David; Hackney, Jennifer F.; Jean-Francois, Michele; Burton, Neal C.; Dobens, Leonard L.

doi:10.1016/j.mod.2007.05.003

Cited by 3 publications

(4 citation statements)

References 61 publications

(68 reference statements)

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“…It has been proposed that slbo/bun/Notch may function as a conserved signaling cassette in regulating cell fate boundaries. In Drosophila , this signaling cassette specifies anterior/posterior identity of follicle cells during oogenesis [ 45 , 76 ]. Bun also functions in restricting Notch activity to the wing margin, producing a notched wing phenotype when mutated [ 76 ].…”

Section: Discussionmentioning

confidence: 99%

“…In Drosophila , this signaling cassette specifies anterior/posterior identity of follicle cells during oogenesis [ 45 , 76 ]. Bun also functions in restricting Notch activity to the wing margin, producing a notched wing phenotype when mutated [ 76 ]. Our finding that bun, slbo and dl were significantly up-regulated during the PO stage suggests this signaling cassette may also be conserved in Lepidoptera, however we did not observe differential expression of Notch.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome analysis of the painted lady butterfly, Vanessa cardui during wing color pattern development

2016

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BackgroundButterfly wing color patterns are an important model system for understanding the evolution and development of morphological diversity and animal pigmentation. Wing color patterns develop from a complex network composed of highly conserved patterning genes and pigmentation pathways. Patterning genes are involved in regulating pigment synthesis however the temporal expression dynamics of these interacting networks is poorly understood. Here, we employ next generation sequencing to examine expression patterns of the gene network underlying wing development in the nymphalid butterfly, Vanessa cardui.ResultsWe identified 9, 376 differentially expressed transcripts during wing color pattern development, including genes involved in patterning, pigmentation and gene regulation. Differential expression of these genes was highest at the pre-ommochrome stage compared to early pupal and late melanin stages. Overall, an increasing number of genes were down-regulated during the progression of wing development. We observed dynamic expression patterns of a large number of pigment genes from the ommochrome, melanin and also pteridine pathways, including contrasting patterns of expression for paralogs of the yellow gene family. Surprisingly, many patterning genes previously associated with butterfly pattern elements were not significantly up-regulated at any time during pupation, although many other transcription factors were differentially expressed. Several genes involved in Notch signaling were significantly up-regulated during the pre-ommochrome stage including slow border cells, bunched and pebbles; the function of these genes in the development of butterfly wings is currently unknown. Many genes involved in ecdysone signaling were also significantly up-regulated during early pupal and late melanin stages and exhibited opposing patterns of expression relative to the ecdysone receptor. Finally, a comparison across four butterfly transcriptomes revealed 28 transcripts common to all four species that have no known homologs in other metazoans.ConclusionsThis study provides a comprehensive list of differentially expressed transcripts during wing development, revealing potential candidate genes that may be involved in regulating butterfly wing patterns. Some differentially expressed genes have no known homologs possibly representing genes unique to butterflies. Results from this study also indicate that development of nymphalid wing patterns may arise not only from melanin and ommochrome pigments but also the pteridine pigment pathway.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2586-5) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis of the painted lady butterfly, Vanessa cardui during wing color pattern development

2016

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“…Human GILZ (hGILZ) codes for a 135-aa protein (39). GILZ has a good homology with other members of the TGF-␤-stimulated clone 22 (TSC-22) family of LZ proteins, which includes at least six additional members (TSC-22, THG-1, KIAA0669, DIP, shc, and Drosophilabunched gene) (40). Mouse GILZ consists of a central LZ (76-97 aa); an N-terminal domain (N-Ter; 1-75 aa), which includes a tuberous sclerosis complex box (TSCbox; 61-75 aa); and a C-terminal, proline, and glutamic acid-rich region (PER; 98-137 aa) ( Fig.…”

Section: Structure and Basic Regulation Of Gilz Expressionmentioning

confidence: 99%

Glucocorticoid‐induced leucine zipper (GILZ): a new important mediator of glucocorticoid action

2009

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Glucocorticoids (GCs) represent the mainstay of current anti-inflammatory and immunosuppressive strategies, mediating effects that mostly result in transcriptional regulation of glucocorticoid receptor target genes. A variety of actions are tied together in the response to GC treatment. Dissecting the beneficial from the detrimental actions in GC therapy is a major challenge in basic research, raising the critical issue of whether a single target gene or gene family might eventually be linked to a specific GC function. Glucocorticoid-induced leucine zipper (GILZ) was originally discovered in studies aimed at characterizing genes targeted by dexamethasone. The first suggestion that GILZ plays an important role in GC immunomodulation came from observations of GILZ up-regulation by GCs, mainly in lymphoid organs, and inhibition of anti-CD3-induced activation and apoptosis. The identification of GILZ interaction with and inhibition of NF-kappaB provided a first molecular mechanistic basis for explaining GILZ effects on T cells. Subsequently, other GILZ targets have been identified, including AP-1, Raf-1, and Ras, all involved in GC effects. The finding that GILZ silencing abrogates the antiproliferative activity of dexamethasone and reduces GC inhibition of cytokine-induced COX-2 expression clearly gained GILZ a distinguished reputation within the critical mediators of GC effects. The multiple functions of GILZ and their potential biological relevance are here reviewed.

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“…. Table S5 [37,190,73,80,157,137,132,154,88,61,194,152] [54,8,30,12,4,143,208,125,195,170] Lbp- (7,8,6,5,9) intracellular fatty acid binding protein (iFABP) predicted to function as an intracellular transporter for small hydrophobic molecules such as lipids and steroid hormones [2,151,147] ake-102 FBgn0036318…”

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confidence: 99%

Concordant developmental expression profiles of orthologues in highly divergent Bilateria

Ferretti

Krämer-Eis

Schiffer

2019

Preprint

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Bilateria are the predominant clade of animals on earth. Despite having evolved a large variety of body-plans and developmental modes, they are characterized by common morphological traits. However, it is not clear if clade-specific genes can be linked to these traits, distinguishing bilaterians from non-bilaterians, with their less complex body morphology. Comparing proteomes of bilaterian and non-bilaterian species in an elaborate computational pipeline we aimed to find and define a set of of bilaterian-specific genes. Finding no high-confidence set of such genes, we nevertheless detected an evolutionary signal possibly uniting the highly diverse bilaterian taxa. Using a novel multi-species GO-enrichment method, we determined the functional repertoire of genes that are widely conserved among Bilateria. We found that these genes contribute to morphogenesis, neuronal-system and muscle development, processes that have been described as di↵erent between bilaterians and non-bilaterians. Analyzing gene expression profiles in three very distantly related bilaterina species, we find characteristic peaks at comparable stages of development and a delayed onset of expression in embryos. In particular, the expression of the conserved genes appears to peak at the phylotypic stage of di↵erent bilaterian phyla. In summary, our data underpin ⇤ corresponding authors the orthologue conjecture and illustrate how development connects distantly related Bilateria after millions of years of divergence, pointing to processes potentially separating them from non-bilaterians. 93 filtering criteria. 94 2.2. Orthologues conserved across divergent Bilateria 95 In our analysis, we included the highly divergent bilaterian model or-96 ganisms Caenorhabditis elegans, Drosophila melanogaster, and Danio rerio.97 4These have very well curated and annotated genomes and are therefore help-98 ful to find genes which are also functionally conserved since more than 99 500Myrs of independent evolution. To take advantage of this, we decided 100 to use the intersection of sets M and L (termed L 0 = L \ M ) for further 101 analysis. Compared to set L (125 clusters), this intersection lacks 31 clusters 102 missing a C. elegans ortholog and 9 clusters missing an ortholog from D. 103 melanogaster, resulting in a set of 85 orthogroups. Except for A. californica, 104 the least represented species in these clusters is S. purpuratus (genes of this 105 species occur in 63 of 85 clusters). By construction the model organisms (C. 106 elegans, D. melanogaster, D. rerio) are represented in all clusters in L 0 . On 107 average, a (bilaterian) species is represented in 93.1% of the clusters.108 2.3. Clade-specificity declines with data availability 109 A general drawback of our experimental procedure is its reliance on cor-110 rectly identified and annotated genes. Since the majority of the organisms 111 in our study are non-model organisms, they may su↵er from incomplete or 112 erroneous gene annotation. For example, we omitted the bilaterian species 113 A. californic...

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A dominant negative allele of the Drosophila leucine zipper protein Bunched blocks bunched function during tissue patterning

Cited by 3 publications

References 61 publications

Transcriptome analysis of the painted lady butterfly, Vanessa cardui during wing color pattern development

Transcriptome analysis of the painted lady butterfly, Vanessa cardui during wing color pattern development

Glucocorticoid‐induced leucine zipper (GILZ): a new important mediator of glucocorticoid action

Concordant developmental expression profiles of orthologues in highly divergent Bilateria

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