A DNA tetrahedron-based molecular beacon for tumor-related mRNA detection in living cells

Xie, Nuli; Huang, Jin; Yang, Xiaohai; Yang, Yanjing; Quan, Ke; He, Wei; Ying, Le; Ou, Min; Wang, Kemin

doi:10.1039/c5cc09980c

Cited by 94 publications

(72 citation statements)

References 41 publications

(3 reference statements)

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“…29–32 In fact, a few DNA tetrahedron-based probes have been assembled for sensing intracellular RNA. 29,33,34 Nevertheless, all of them are single-intensity-based sensing probes, which may lead to high false-positive signals from nuclease digestion, protein binding, or thermodynamic fluctuations. 21 Moreover, such sensing is compromised by the local distribution of probes and by drifts of light sources and detectors.…”

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confidence: 99%

Fluorescence Resonance Energy Transfer-Based DNA Tetrahedron Nanotweezer for Highly Reliable Detection of Tumor-Related mRNA in Living Cells

et al. 2017

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Accurate detection and imaging of tumor-related mRNA in living cells hold great promise for early cancer detection. However, currently, most probes designed to image intracellular mRNA confront intrinsic interferences arising from complex biological matrices and resulting in inevitable false-positive signals. To circumvent this problem, an intracellular DNA nanoprobe, termed DNA tetrahedron nanotweezer (DTNT), was developed to reliably image tumor-related mRNA in living cells based on the FRET (fluorescence resonance energy transfer) “off” to “on” signal readout mode. DTNT was self-assembled from four single-stranded DNAs. In the absence of target mRNA, the respectively labeled donor and acceptor fluorophores are separated, thus inducing low FRET efficiency. However, in the presence of target mRNA, DTNT alters its structure from the open to closed state, thus bringing the dual fluorophores into close proximity for high FRET efficiency. The DTNT exhibited high cellular permeability, fast response and excellent biocompatibility. Moreover, intracellular imaging experiments showed that DTNT could effectively distinguish cancer cells from normal cells and, moreover, distinguish among changes of mRNA expression levels in living cells. The DTNT nanoprobe also exhibits minimal effect of probe concentration, distribution and laser power as other ratiometric probe. More importantly, as a result of the FRET “off” to “on” signal readout mode, the DTNT nanoprobe almost entirely avoids false-positive signals due to intrinsic interferences, such as nuclease digestion, protein binding and thermodynamic fluctuations in complex biological matrices. This design blueprint can be applied to the development of powerful DNA nanomachines for biomedical research and clinical early diagnosis.

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confidence: 99%

Fluorescence Resonance Energy Transfer-Based DNA Tetrahedron Nanotweezer for Highly Reliable Detection of Tumor-Related mRNA in Living Cells

et al. 2017

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“…Xie et al, on the other hand, described a DNA tetrahedron that incorporated a flurophore and quencher at the end of one of the strands making up the structure, thus putting the two moieties in close proximity when the tetrahedron is in the intact form . Target binding induces a structural change that separates the fluorophore and quencher, turning on fluorescence.…”

Section: Hybridization‐based Probesmentioning

confidence: 99%

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

2019

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The chemical composition of cells at the molecular level determines their growth, differentiation, structure, and function. Probing this composition is powerful because it provides invaluable insight into chemical processes inside cells and in certain cases allows disease diagnosis based on molecular profiles. However, many techniques analyze fixed cells or lysates of bulk populations, in which information about dynamics and cellular heterogeneity is lost. Recently, nucleic‐acid‐based probes have emerged as a promising platform for the detection of a wide variety of intracellular analytes in live cells with single‐cell resolution. Recent advances in this field are described and common strategies for probe design, types of targets that can be identified, current limitations, and future directions are discussed.

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“…Given its low cytotoxicity, high resistance to enzymatic degradation, and cellular permeability, Xie el al. designed a FNA‐based molecular beacon for mRNA detection in living cells . Inspired by “protective‐yet‐accessible” exoskeletons, Leong group developed termed nano‐snail‐inspired nucleic acid locator (nano‐SNEL) as a mRNA nanosensor (Figure a), which is composed of a sensory molecular beacon module for detecting mRNA and a DNA nanoshell component for protecting the sensory component from nuclease degradation.…”

Section: Intracellular Sensingmentioning

confidence: 99%

“…designed a FNA-based molecular beacon for mRNA detection in living cells. [69] Inspired by "protective-yet-accessible" exoskeletons, Leong group developed termed nano-snail-inspired nucleic acid locator (nano-SNEL) as a mRNA nanosensor (Figure 8a), [70] which is composed of a sensory molecular beacon module for detecting mRNA and a DNA nanoshell component for protecting the sensory component from nuclease degradation. They demonstrated that this nano-SNEL as an intracellular probe enables accurate and sensitive visualization of mRNA.…”

Section: Intracellular Sensingmentioning

confidence: 99%

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

Liu

et al. 2019

ChemPlusChem

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With the advent of DNA nanotechnology, nucleic acids have been used building blocks for constructing various DNA nanostructures. As classical and simple DNA nanostructures, framework nucleic acids (FNAs) have attracted enormous attention in the field of biosensing. The sequence‐specific self‐assembly properties and high programmability of nucleic acids allow FNAs to be incorporated in advanced probe design. In addition, FNAs enable the engineering of surfaces for biosensing (e. g., probe orientation, probe spacing), thereby improving the accessibility of target molecules to the probes arranged on heterogeneous surfaces. Moreover, FNAs offer a universal and promising platform for sensing cellular molecules because of their prominent biocompatibility and cellular permeability. Herein recent advances in FNA‐based biosensors are summarized, including electrochemical detection, optical detection, and intracellular sensing. It is hoped that this review will provide guidelines for the design and construction of FNA scaffold‐based biosensors.

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A DNA tetrahedron-based molecular beacon for tumor-related mRNA detection in living cells

Cited by 94 publications

References 41 publications

Fluorescence Resonance Energy Transfer-Based DNA Tetrahedron Nanotweezer for Highly Reliable Detection of Tumor-Related mRNA in Living Cells

Fluorescence Resonance Energy Transfer-Based DNA Tetrahedron Nanotweezer for Highly Reliable Detection of Tumor-Related mRNA in Living Cells

Nucleic‐Acid Structures as Intracellular Probes for Live Cells

Rational Design of Framework Nucleic Acids for Bioanalytical Applications

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