A DNA barcode for Piroplasmea

Gou, Huitian; Guan, Guiquan; Liu, Aihong; Ma, Miling; Xu, Zongke; Liu, Zhijie; Ren, Qiaoyun; Li, Youquan; Yang, Jifei; Chen, Ze; Yin, Hong; Luo, Jianxun

doi:10.1016/j.actatropica.2012.07.001

Cited by 25 publications

(15 citation statements)

References 39 publications

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“…The gene encoding the 18S rRNA is more conservative than is COI , and has a slower evolutionary rate (Gou et al. ; Nassonova et al. ).…”

Section: Discussionmentioning

confidence: 99%

“…This discrepancy might be because of the difference in evolutionary rate of the two genes. The gene encoding the 18S rRNA is more conservative than is COI, and has a slower evolutionary rate (Gou et al 2012;Nassonova et al 2010). Published divergence time of ticks ranges across two evolutionary epochs, including the Triassic (248-206 Mya) (Balashov 1989(Balashov , 1994 and Cretaceous (146-65 Mya) (Black and Piesman 1994).…”

Section: Discussionmentioning

confidence: 99%

“…MtDNA offers several advantages in comparison to nuclear DNA: rapid evolution, limited exposure to recombination, lack of introns, and high copy number. These characteristics of mtDNA are important for routine amplification by polymerase chain reaction (PCR) and use as a molecular marker for phylogeny (Gou et al 2012). In this study, we used the cytochrome oxidase I (COI) gene to calculate the molecular clock of piroplasmids and hard ticks, and also reconstructed molecular phylogenies for the parasites and their hosts to assess the extent of coevolution in this association.…”

Section: Introductionmentioning

confidence: 99%

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Coevolutionary analyses of the relationships between piroplasmids and their hard tick hosts

Gou

Guan

Liu

et al. 2013

Ecology and Evolution

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Host–parasite coevolution is a key driver of biological diversity. To examine the evolutionary relationships between piroplasmids and their hard tick hosts, we calculated the molecular clock and conducted phylogenetic analyses of both groups. Based on our results, we conclude that the divergence time of piroplasmids (∼56 Mya) is later than divergence time of their hard tick hosts (∼86 Mya). From analyses of the evolution of both piroplasmid and vector lineages and their association, we know that hard ticks transmit piroplasmids with high genus specificity and low species specificity.

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“…The gene encoding the 18S rRNA is more conservative than is COI , and has a slower evolutionary rate (Gou et al. ; Nassonova et al. ).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Coevolutionary analyses of the relationships between piroplasmids and their hard tick hosts

Gou

Guan

Liu

et al. 2013

Ecology and Evolution

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“…For positive samples, modified nested PCR amplifying ~1700 bp fragments of 18S rDNA using the combination of BT1 F, BT outer R primers in first run and BT Inner R [20] and Piro0F2 [21] primers in second run was used. To confirm the determination of piroplasmids and to obtain further phylogenetic characterization, two modified nested PCR protocols amplifying fragments of cytochrome c oxidase subunit 1 ( cox 1 ) gene were performed [21, 22]. Table 1 provides details on the primers used, PCR conditions and obtained fragments for all PCRs.…”

Section: Methodsmentioning

confidence: 99%

“…To confirm the determination of piroplasmids and to obtain further phylogenetic characterization, two modified nested PCR protocols amplifying fragments of cytochrome c oxidase subunit 1 ( cox 1 ) gene were performed [21, 22]. Table 1 provides details on the primers used, PCR conditions and obtained fragments for all PCRs.…”

Section: Methodsmentioning

confidence: 99%

Eurasian golden jackal as host of canine vector-borne protists

et al. 2017

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BackgroundJackals are medium-sized canids from the wolf-like clade, exhibiting a unique combination of ancestral morphotypes, broad trophic niches, and close phylogenetic relationships with the wolf and dog. Thus, they represent a potential host of several pathogens with diverse transmission routes. Recently, populations of the Eurasian golden jackal Canis aureus have expanded into the Western Palaearctic, including most of Europe. The aim of our study was to examine Eurasian golden jackals from Romania, Czech Republic and Austria for a wide spectrum of vector-borne protists and to evaluate the role of this species as a reservoir of disease for domestic dogs and/or humans.ResultsDiagnostic polymerase chain reaction (PCR) DNA amplifications revealed 70% of jackals to be positive for Hepatozoon, 12.5% positive for piroplasms, and one individual positive for Leishmania infantum. Phylogenetic analyses of partial 18S rDNA sequences invariably placed sequenced isolates of Hepatozoon into the H. canis clade. For piroplasms, both the 18S and cox1 sequences obtained confirmed the presence of Babesia canis and “Theileria annae” in 5 and 2 individuals, respectively, providing the first records of these two piroplasmids in Eurasian golden jackals. A single animal from Dolj County (Romania) was PCR-positive for L. infantum, as confirmed also by sequencing of ITS1-5.8S.ConclusionsApparently, expanding populations of jackals can play a significant role in spreading and maintaining new Babesia canis foci in Central Europe. The role of jackals in the epidemiology of “Theileria annae” and H. canis is probably similar to that of red foxes and should be taken into account in further research on these parasites. Also the presence of L. infantum deserves attention. Our study confirms that once established, the populations of Eurasian golden jackals constitute natural reservoirs for many canine vector-borne diseases, analogous to the role of the coyotes in North America.

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DNA barcoding to map the microbial communities: current advances and future directions

Chakraborty

Doss

Patra

et al. 2014

Appl Microbiol Biotechnol

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During the last two decades, the DNA barcode development towards microbial community has increased dramatically. DNA barcode development is related to error-free and quick species identification which aid in understanding the microbial biodiversity, as well as the diseases related to microbial species. Here, we seek to evaluate the so-called barcoding initiatives for the microbial communities and the emerging trends in this field. In this paper, we describe the development of DNA marker-based DNA barcoding system, comparison between routine species identification and DNA barcode, and microbial biodiversity and DNA barcode for microbial communities. Two major topics, such as the molecular diversity of viruses and barcode for viruses have been discussed at the same time. We demonstrate the current status and the maker of DNA barcode for bacteria, algae, fungi, and protozoa. Furthermore, we argue about the promises, limitations, and present and future challenges of microbial barcode development.

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A DNA barcode for Piroplasmea

Cited by 25 publications

References 39 publications

Coevolutionary analyses of the relationships between piroplasmids and their hard tick hosts

Coevolutionary analyses of the relationships between piroplasmids and their hard tick hosts

Eurasian golden jackal as host of canine vector-borne protists

DNA barcoding to map the microbial communities: current advances and future directions

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