A distinct tymovirus infecting Cassia hoffmannseggii in Brazil

Nicolini, Cícero; Pio-Ribeiro, Gilvan; Andrade, Gilcifran Prestes de; Melo, Fernando Lucas; Oliveira, Virgínia Carla de; Guimarães, Felipe Coutinho; Resende, Renato O.; Kitajima, Elliot Watanabe; Rezende, J. A. M.; Nagata, Tatsuya

doi:10.1007/s11262-012-0750-9

Cited by 11 publications

(8 citation statements)

References 19 publications

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“…The sequences and characteristics of all primers used in this study are summarized in Table S1 . To determine the complete genome sequence of BaCV-Luz, first we determined the 5′ and 3′ ends by rapid amplification of cDNA ends (RACE) as previously described [ 6 , 12 , 13 ]. Next, based on the genome sequence of BaCV-GO [ 6 ] and BaCV-Luz leader and trailer sequences, primers were designed to amplify the virus genome by RT-PCR in six fragments overlapping in at least 150 nt.…”

Section: Methodsmentioning

confidence: 99%

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Pinheiro-Lima

Pereira–Carvalho

Alves-Freitas

et al. 2020

Viruses

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The knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown. This information is crucial for the understanding of virus–plant interactions, control strategies, and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plants and are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers, and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papaya cytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil, (ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here, we examine the potential of the Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) to transmit BaCV, its morphological and cytopathological characteristics, and assess the incidence of BaCV across bean producing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimental conditions, by B. tabaci MEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean. Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viral particles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests, identification of the induced symptoms, and the transmission assay. BaCV was detected in five out of the seven states in Brazil included in our study, suggesting that it is widely distributed throughout bean producing areas in the country. This is the first report of a whitefly-transmitted rhabdovirus.

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Section: Methodsmentioning

confidence: 99%

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

Pinheiro-Lima

Pereira–Carvalho

Alves-Freitas

et al. 2020

Viruses

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“…To determine the 5′- and 3′-terminal sequences of BaCV genome, rapid amplification of cDNA ends (RACE) was performed according to the method described previously [18,19]. The 3′ end was confirmed by the addition of a poly-A tail to the total RNA using the Escherichia coli poly(A) polymerase (New England Biolabs, Ipswich, MA, USA); then, the cDNA was synthesized using an oligo(dT) primer with an anchor sequence, M10PacIT50VN (Table S1).…”

Section: Methodsmentioning

confidence: 99%

Double-Stranded RNA High-Throughput Sequencing Reveals a New Cytorhabdovirus in a Bean Golden Mosaic Virus-Resistant Common Bean Transgenic Line

Alves-Freitas

Pinheiro-Lima

Faria

et al. 2019

Viruses

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Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15–39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean.

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“…To date, only five tymoviruses infecting different plant species were reported in Brazil: (i) cassia yellow mosaic-associated virus (2), (ii) eggplant mosaic virus (EMV) (3, 4), (iii) passion fruit yellow mosaic virus (5), (iv) petunia vein banding virus (6), and (v) tomato blistering mosaic virus (ToBMV) (7). Most of these viruses were identified as distinct tymoviruses based on biological, biochemical, and serological properties, and no complete genomic RNA sequences are available for these viruses, except for the recently described ToBMV (7) (GenBank accession no.…”

Section: Genome Announcementmentioning

confidence: 99%