Complete Genome Sequence of a Tobacco-Infecting, Tomato-Blistering Mosaic Virus

Melo, Fernando Lucas; Fernandes, J. E. A.; Ribeiro, Bergmann Morais; Ribeiro, S. G.

doi:10.1128/genomea.00701-14

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…ToBMV was isolated from Santa Catarina state, Brazil and tentatively named as tomato blistering mosaic virus (ToBMV) [7]. ToBMV was also able to infect several other plants, including Nicotiana tabacum, N. benthamiana, Capsicum annuum and Solanum violaefolium [8,9]. This virus species was always found in virome studies in tomato and pepper plants using Next-generation sequencing (Tatsuya Nagata, unpublished data) and, therefore it is essential to investigate and understand the effects of this virus in the host plant since it represents a serious threat to tomato and pepper producing areas.…”

Section: Introductionmentioning

confidence: 99%

“…A phylogenetic analysis was also performed and showed that ToBMV clustered with other common tymoviruses that infect solanaceous hosts [6,7]. Recently, the complete genome of ToBMV was sequenced [6,8,9] revealing a size of about 6.3 kb and three ORFs: ORF 1 encodes the RNA replication polyprotein, (cleaved to metyltransferase/protease, helicase, and RNAdependent RNA polymerase), ORF 2 the movement protein, and ORF 3 the coat protein. ToMBV causes the typical cytopathic effects usually seen on tymovirus infected tissue, including double-membrane vesicles on the periphery of the chloroplasts [7].…”

Section: Introductionmentioning

confidence: 99%

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Chloroplast Proteome of Nicotiana benthamiana Infected by Tomato Blistering Mosaic Virus

et al. 2018

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Tymovirus is a genus of plant pathogenic viruses that infects several dicotyledonous plants worldwide, causing serious diseases in economically important crops. The known cytopathic effect on the host cell organelles involves chloroplast membrane deformation and the induction of vesicles in its periphery. These vesicles are known to be the location where tymoviral genomic RNA replication occurs. Tomato blistering mosaic virus (ToBMV) is a tymovirus recently identified in tomato plants in Brazil, which is able to infect several other plants, including tobacco. In this work, we investigated the chloroplast proteomic profile of ToBMV-infected N. benthamiana using bidimensional electrophoresis (2-DE) and mass spectrometry, aiming to study the virus-host interaction related to the virus replication and infection. A total of approximately 200 spots were resolved, out of which 36 were differentially abundant. Differential spots were identified by mass spectrometry including photosynthesis-related and defense proteins. We identified proteins that may be targets of a direct interaction with viral proteins, such as ATP synthase β subunit, RNA polymerase beta-subunit, 50S ribosomal protein L6 and Trigger factor-like protein. The identification of these candidate proteins gives support for future protein-protein interaction studies to confirm their roles in virus replication and disease development.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chloroplast Proteome of Nicotiana benthamiana Infected by Tomato Blistering Mosaic Virus

et al. 2018

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“…Tobacco (Nicotiana tabacum) plants in Brazil have been described in the last decade as hosts for a variety of viruses, and special attention has been paid to potyviral and tymoviral infections [9,12,13]. Three potyviruses have been found infecting solanaceous plants in Brazil: pepper yellow mosaic virus (PepYMV) [4,6], potato virus Y (PVY) [3], and Brugmansia suaveolens mottle virus (BsMoV) [7].…”

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confidence: 99%

Complete genome sequence of tobacco mosqueado virus

et al. 2016

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We describe the genomic characteristics of a new potyvirus isolated from tobacco plants showing mottling ("mosqueado" in Portuguese) in southern Brazil. The complete genomic sequence consists of 9896 nucleotides, without the poly(A) tail, and shares the highest pairwise nucleotide sequence identities of 68.5 % with pepper yellow mosaic virus and 68.2 % with Brugmansia mosaic virus isolate D437. These identity values are below the level of 76.0 % used as a criterion for species demarcation in the genus Potyvirus based on the complete genome sequence. The viral genomic organization and sequence comparison thus suggest that this virus, tentatively named "tobacco mosqueado virus" (TMosqV), represents a new potyvirus species.

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