A distinct stratum corneum antigen in psoriasis and its reactions with stratum corneum autoantibodies

Abstract

Qutaishat, S. S., Kumar, V., Beutner, E. H. & Jablonska, S. A distinct stratum corneum antigen in psoriasis and its reactions with stratum corneum autoantibodies. APMIS 100: 341-346, 1992. Stratum corneum antibodies are ubiquitous and can be detected by various immunological methods. Of these, the ones detected by hemagglutination undergo changes in antibody titers and have been implicated in psoriasis. The purpose of our study was to examine if differences exist in the activities of the antigens isolated from psoriatic scales in comparison to normal callus. Stratum corneum antigens were prepared by trypsin-phenol-water extraction. The water phase, which contains the stratum corneum antigen, was used to sensitize the red blood cells in the hernagglutination assay. The antibody activity in human sera was determined before and after absorption with antigens isolated from callus, psoriatic scales, and cell envelopes. We found notable differences in the antigens obtained from callus and psoriatic scales. These include higher antibody titers to the antigens of the scales, the presence of unique antigenic determinants on psoriatic scales and the localization of the antigen on cell envelopes. These immunological differences were corroborated by the marked biochemical differences of certain amino acids, most notably glycine and proline, and these differences were unique to psoriatic scales as they were not shared with other hyperproliferative disorders.

Immune reactivity in psoriatic Munro-Saboureau microabscesses, stratum corneum and blood vessels

“…[58] It has been shown via the mixed agglutination technique on tissue sections that 25 patient's psoriatic lesions contained immunoglobulins of the IgG and IgM classes in the epidermis. The authors estimated the maximum immunoglobulin concentrations were around the dermal-epidermal junction, and above the dermal papillae.…”

Discovery of natural bispecific antibodies: Is psoriasis induced by a toxigenic Corynebacterium simulans and maintained by CIDAMPs as autoantigens?

“…Thus, it is tempting to speculate that a CIDAMP‐based, persisting epithelial defence reaction within the stratum corneum and stratum granulosum towards virulent C. simulans could eventually result in the generation of CIDAMP‐like autoantigens. Since CIDAMPs are generated from cationic IDP(R)s, which are highly abundant in keratinocytes, 37 and are produced within the fully differentiated epidermal layers, it would be plausible to see CIDAMP‐directed immune reactions, such as IgG‐deposits, within the stratum corneum 3,66 and autoreactive T‐cell‐mediated 67,68 responses within the stratum granulosum. Keratinocyte‐derived CIDAMPS as autoantigens would also help to explain the typical gyrate, demarcated lesion morphology of annular psoriasis, 69 where I would propose that the centre is missing CIDAMP sources due to a loss of the stratum granulosum.…”

Do immune responses to inhaled skin flakes modulate the expression of allergic disease?

“…Additionally, in a small study using serum from five 18‐month‐old infants, we found that between ∼30% and 50% of skin‐like particles in their bed dust eluted antigens that bound their IgG (unpublished observations). Indeed, there is a long history of observations in this area [33–36]. Extracts of normal SC have been demonstrated to contain undescribed T cell antigens of high potency but limited structural diversity [37]; these appear to be analogous in effect to superantigens but require MHC Class II presentation.…”