A direct competitive nanozyme-linked immunosorbent assay based on MnO₂ nanosheets as a catalytic label for the determination of fumonisin B₁

Abstract

A direct competitive nanozyme-linked immunosorbent assay (dcNLISA) based on MnO2 nanosheets (MnO2 NSs) as a nanozyme label was developed for the highly sensitive determination of fumonisin B1 (FB1).

Establishment of fumonisin B1 detection method for catalytic fluorescence detection of aptamer-regulated carbon dots

“…29 Cai et al developed a direct competitive nano-enzyme-linked immunosorbent assay for the determination of FB 1 with an LOD of 0.63 ng mL −1 . 30 Although the LOD of this method is not comparable to the above methods, it meets the requirements of the EU and FDA, and the method has a wide detectable range of FB 1 , short detection time and low detection cost.…”

A comprehensive exploration of the latest innovations for advancements in enhancing selectivity of nanozymes for theranostic nanoplatforms

“…Additionally, the unique catalytic properties of nanozymes can result in reduced background noise and improved signal-to-noise ratios, enhancing the assay's specificity. 83 The flexibility to choose from various detection methods adds versatility to NLISA, making it adaptable to different experimental setups and sample types. 84,85 For instance, Li et al devised an innovative method named the nanozyme-linked immunosorbent surface plasmon resonance (nano-ELISPR) biosensor, designed for highly sensitive cancer biomarker detection.…”