A direct comparison between membrane adsorber and packed column chromatography performance

Boi, Cristiana; Malavasi, A.; Carbonell, Ruben G.; Gilleskie, Gary

doi:10.1016/j.chroma.2019.460629

Cited by 49 publications

(44 citation statements)

References 39 publications

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“…However, many chromatographic cycles were performed for each support, changing the initial BSA concentration and the flow rate; more data can be found in the Supplementary Material, Figures S1 and S2. The results reported here and in the Supplementary Material confirm the characteristic behavior of convective chromatographic materials, in that the performance of convective stationary phases is much less affected by flow rate than packed columns as indicated in the literature [5,6,[31][32][33][34][35][36][37][38][39]. The influence of flow rate on the shape of the breakthrough curves for all stationary phases studied can be observed in Figure 4.…”

Section: Dynamic Binging Capacitysupporting

confidence: 85%

Affinity Membranes and Monoliths for Protein Purification

et al. 2019

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Affinity capture represents an important step in downstream processing of proteins and it is conventionally performed through a chromatographic process. The performance of this step highly depends on the type of matrix employed. In particular, resin beads and convective materials, such as membranes and monoliths, are the commonly available supports. The present work deals with non-competitive binding of bovine serum albumin (BSA) on different chromatographic media functionalized with Cibacron Blue F3GA (CB). The aim is to set up the development of the purification process starting from the lab-scale characterization of a commercially available CB resin, regenerated cellulose membranes and polymeric monoliths, functionalized with CB to identify the best option. The performance of the three different chromatographic media is evaluated in terms of BSA binding capacity and productivity. The experimental investigation shows promising results for regenerated cellulose membranes and monoliths, whose performance are comparable with those of the packed column tested. It was demonstrated that the capacity of convective stationary phases does not depend on flow rate, in the range investigated, and that the productivity that can be achieved with membranes is 10 to 20 times higher depending on the initial BSA concentration value, and with monoliths it is approximately twice that of beads, at the same superficial velocity.

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Section: Dynamic Binging Capacitysupporting

confidence: 85%

Affinity Membranes and Monoliths for Protein Purification

et al. 2019

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“…Figure 8 illustrates the performance of PIP, PIP-PEI, and PIP-Z membranes during the dynamic fouling experiments using both 100 ppm BSA and 100 ppm Lysozyme solutions separately as model protein foulants along with 1000 ppm Na 2 SO 4 at pH 7 for an operational time up to 14 h. As evident from Figure 8, the normalized flux for all the membranes varied in the range of 1.0-0.8 for both the model protein foulants. The isoelectric point (pI) of BSA and lysozyme is 4.7 and 11.0, individually [55]. The positively charged PIP-PEI membrane exhibited lower normalized flux for the negatively charged BSA as compared to the PIP membranes.…”

Section: Antifouling and Antibacterial Behaviormentioning

confidence: 99%

Zwitterion Co-Polymer PEI-SBMA Nanofiltration Membrane Modified by Fast Second Interfacial Polymerization

et al. 2020

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Nanofiltration membranes have evolved as a promising solution to tackle the clean water scarcity and wastewater treatment processes with their low energy requirement and environment friendly operating conditions. Thin film composite nanofiltration membranes with high permeability, and excellent antifouling and antibacterial properties are important component for wastewater treatment and clean drinking water production units. In the scope of this study, thin film composite nanofiltration membranes were fabricated using polyacrylonitrile (PAN) support and fast second interfacial polymerization modification methods by grafting polyethylene amine and zwitterionic sulfobutane methacrylate moieties. Chemical and physical alteration in structure of the membranes were characterized using methods like ATR-FTIR spectroscopy, XPS analysis, FESEM and AFM imaging. The effects of second interfacial polymerization to incorporate polyamide layer and ‘ion pair’ characteristics, in terms of water contact angle and surface charge analysis was investigated in correlation with nanofiltration performance. Furthermore, the membrane characteristics in terms of antifouling properties were evaluated using model protein foulants like bovine serum albumin and lysozyme. Antibacterial properties of the modified membranes were investigated using E. coli as model biofoulant. Overall, the effect of second interfacial polymerization without affecting the selectivity layer of nanofiltration membrane for their potential large-scale application was investigated in detail.

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“…The ion exchangers were initially 3D printed as hollow cylinders ( Figure 1c-e), and adsorption of pure Bovine Serum Albumin (BSA) and lysozyme (LYS) was measured in batch conditions on the AETAC and CEA materials, respectively. Maximum binding capacities of 104.2 ± 10.6 mg of BSA per mL of AETAC-based support, and 108.1 ± 25.9 mg of LYS per mL of CEA-based material were recorded (Figure 2a and 2b), about 5 fold higher than for commercial monoliths (Hahn et al, 2002) and chromatographic membranes (Boi et al, 2020) and in line or above standard chromatographic resins (Staby et al, 2005). Testing in dynamic conditions was carried out using Schoen gyroid columns ( Figure 1f-h) by loading BSA and myoglobin (MYO) onto the AETAC material ( Figure 2c, Simon et al, 2020) and BSA and LYS on the CEA material ( Figure 2d).…”

Section: Introductionmentioning

confidence: 92%

“…Isotherms were fitted with Langmuir model (continuous lines). Dashed lines corresponds to maximum binding capacity of equivalent commercial materials (Boi et al, 2020;Staby et al, 2005). (c) Separation of BSA (16 mg/mL) and myoglobin (MYO, 6 mg/mL) on AETAC-based anion exchangers (1.6 mL column volume (CV), 1.73 mmol/mL ligand density).…”

Section: Acknowledgementsmentioning

confidence: 99%

Flexible material formulations for 3D printing of porous beds with applications in bioprocess engineering

Dimartino

Rodriguez

Simon

et al. 2021

Preprint

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3D printing is revolutionizing many industrial sectors and has the potential to enhance also the biotechnology and bioprocessing fields. Here, we propose a new flexible material formulation to 3D print support matrices with complex, perfectly ordered morphology and with tuneable properties to suit a range of applications in bioprocess engineering. Supports for packed-bed operations were fabricated using functional monomers as the key ingredients, enabling matrices with bespoke chemistry such as charged groups, chemical moieties for further functionalization, and hydrophobic/hydrophilic groups. Other ingredients, e.g. crosslinkers and porogens, provide the opportunity to further tune the mechanical properties of the supports and the morphology of their porous network. Through this approach, we fabricated and demonstrated the operation of Schoen gyroid columns with I) positive and negative charges for ion-exchange chromatography, II) enzyme bioreactors with immobilized trypsin to catalyse hydrolysis, and III) bacterial biofilms bioreactors for fuel desulfurization. We expect this approach will enable simple, cost-effective and flexible fabrication of customized supports in biotechnology and bioengineering.

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A direct comparison between membrane adsorber and packed column chromatography performance

Cited by 49 publications

References 39 publications

Affinity Membranes and Monoliths for Protein Purification

Affinity Membranes and Monoliths for Protein Purification

Zwitterion Co-Polymer PEI-SBMA Nanofiltration Membrane Modified by Fast Second Interfacial Polymerization

Flexible material formulations for 3D printing of porous beds with applications in bioprocess engineering

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