Affinity Membranes and Monoliths for Protein Purification

Lalli, Eleonora; Silva, Jouciane S.; Boi, Cristiana; Sarti, Giulio Cesare

doi:10.3390/membranes10010001

Cited by 26 publications

(19 citation statements)

References 57 publications

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“…This technique has been used to immobilize a variety of ligands for AMC. Examples of binding agents that have been coupled by this approach are protein A, protein G, protein L, BSA, HSA, antibodies, amino acids, enzymes, synthetic dyes, boronates, and antibodies [47,50,64,74,132,152,163–172].…”

Section: Immobilization Methods For Affinity Monolithsmentioning

confidence: 99%

Affinity monolith chromatography: A review of general principles and recent developments

2021

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Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix. The target‐specific interaction exhibited by the binding agents makes AMC attractive for the separation or detection of a wide range of compounds. This article will review the basic principles of AMC and recent developments in this field. The supports used in AMC will be discussed, including organic, inorganic, hybrid, carbohydrate, and cryogel monoliths. Schemes for attaching binding agents to these monoliths will be examined as well, such as covalent immobilization, biospecific adsorption, entrapment, molecular imprinting, and coordination methods. An overview will then be given of binding agents that have recently been used in AMC, along with their applications. These applications will include bioaffinity chromatography, immunoaffinity chromatography, immobilized metal‐ion affinity chromatography, and dye‐ligand or biomimetic affinity chromatography. The use of AMC in chiral separations and biointeraction studies will also be discussed.

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Section: Immobilization Methods For Affinity Monolithsmentioning

confidence: 99%

Affinity monolith chromatography: A review of general principles and recent developments

2021

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“…Мембранные хроматографические системы обычно имеют размеры пор в диапазоне микрофильтрации (0,02-10 мкм), поскольку они не разделяются на основе молекулярного размера и могут обеспечивать высокие скорости потока, низкие перепады давления, легкую упаковку колонки, а также низкий уровень засорения, что делает их идеальными для разделения белков в производственных условиях [94,95].…”

Section: нанофильтрация (нф)unclassified

Whey: State of the Art. Part II. Processes and Treatment Methods

Paladii¹,

Vrabie²,

Sprincean³

et al. 2021

EOM

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The current state of the art on studying of whey is considered. The processes and methods of whey processing (thermal, chemical, physicochemical, biotechnological, and electrophysical) are presented. Thermal and isoelectric precipitation of proteins, using reagents and coagulants, as well as the main membrane processing methods (reverse osmosis, diafiltration, microfiltration, ultrafiltration, and nanofiltration) are described. Possibi-lities of effective separation of whey proteins by a combination of membrane and other methods are noted. Chromatographic methods for fractionation of whey proteins (chromatography with imitation of a moving bed, high-gradient chromatography with a magnetic trap, selective adsorption, displacement chromatography, membrane adsorption), which provide a high degree of separation, are described. Highly porous chromatographic materials providing a high flow rate, biotechnological processing methods – biosynthesis of lactulose, enzymatic hydrolysis of lactose and whey proteins, aerobic and anaerobic fermentation are considered. Electrophysical methods of processing whey (electrodialysis, electroactivation), which include electrodialysis and electro-activation, as well as electrochemical activation as a phenomenon and technology, as a new promising processing method that allows to create a waste-free cycle for obtaining valuable components and useful derivatives from whey without the use of reagents are analyzed. It is emphasized that, depending on the regimes used, protein-mineral concentrates with a predetermined protein or mineral composition are obtained with the simultaneous isomerization of lactose into lactulose. It is stated that the efficiency of methods for processing whey is ensured by a significant increase in the efficiency of technological processes, a decrease in labor costs, a reduction in the processing time and materials, and an improvement in the quality and functional properties of the final products.

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“…Several kinds of research have been reported in terms of application of inorganic polymers, such as separation and detection of cation levels in drinking water , protein (Zhao et al, 2012), tryptic digests of bovine serum albumin (BSA) (Zhang et al, 2011), levodopa, carbidopa, benserazide, dopamine, and 3-O-methyldopa in plasma samples (Grecco et al, 2020), polar compounds (Yang et al, 2013) and other macromolecular compounds (Iwasaki et al, 2012). However, time-consuming manufacturing and a limited variety of polymer-forming materials were obtained as disadvantages of inorganic polymer (Zhong et al, 2010, Lalli et al, 2020.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Organic Polymer Monolith Columns Containing Ammonium Quarternary As Initial Study For Capillary Chromatography

Rahayu

Jamilatun

Fajri

et al. 2021

EKW

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The polymerization process with a simple step has become the centre of attention of several researchers. Various polymers have been developed, although in general, they use polymerization with a post-modification method. A quaternary ammonium monolith organic polymer has been prepared using a simple single thermal method in this research. 2-(Methacryloyloxy)-N,N,N-trimethylethanaminium chloride was as the monomer, and ethylene dimethacrylate was as crosslinker. The polymerization proceeded in fused-silica capillary (100 mm, 0.32 mm i.d. x 0.45 mm o.d.) using a one-pot approach method. To achieve the perfect macropores, isopropyl alcohol, PEG 400, and ethanol were used as porogen. Characterization of the surface morphology was carried out using a Scanning Electron Microscope (SEM), and the existence of an amine group was characterized by Fourier Transform Infrared Spectroscopy (FTIR). The distribution size of pores in the polymer was in the range of 1.29 to 3.33 µm.Abstrak: Polimerisasi dengan proses yang sederhana dan simpel menjadi pusat perhatian beberapa peneliti. Berbagai macam polimer telah dikembangkan, akan tetapi pada umumnya menggunakan polimerisasi dengan metode post-modification. Pada penelitian ini, polimer organik yang mengandung amonium kuartener dalam bentuk monolit dengan polimerisasi yang menggunakan suhu tunggal dan sederhana telah dilakukan. 2-(Methacryloyloxy)-N,N,N-trimethylethanaminium chloride digunakan sebagai monomer dan ethylene dimethacrylate sebagai crosslinker. Polimerisasi dilakukan dengan metode one-pot aaproach di dalam kapiler silika (100 mm, 0,32 mm i.d. x 0,45 mm o.d.). Untuk mendapatkan makropori yang sempurna, isopropil alkohol, PEG 400 dan etanol digunakan sebagai porogen. Karakterisasi morfologi permukaan dilakukan dengan menggunakan Scanning Electron Microscope (SEM), dan Fourier Transform Infrared Spectroscopy (FTIR) untuk mengidentifikasi gugus fungsi gugus amina yang terdapat pada polimer. Ukuran distribusi pori pada polimer berkisar antara 1,29 sampai 3,33 µm.

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Affinity Membranes and Monoliths for Protein Purification

Cited by 26 publications

References 57 publications

Affinity monolith chromatography: A review of general principles and recent developments

Affinity monolith chromatography: A review of general principles and recent developments

Whey: State of the Art. Part II. Processes and Treatment Methods

Characterization of Organic Polymer Monolith Columns Containing Ammonium Quarternary As Initial Study For Capillary Chromatography

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