A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1

Mir, Ricardo; Aranda, Leslie Z.; Biaocchi, Tiffany; Luo, Anding; Sylvester, Anne W.; Rasmussen, Carolyn G.

doi:10.1104/pp.16.01454

Cited by 14 publications

(13 citation statements)

References 48 publications

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“…3). Analysis of relative auxin levels during tassel and ear development, perhaps using the DII-VENUS reporter (Mir et al, 2017), could be used to validate these findings in planta and provide possible clues to the impact of increased sensitivity on feedback within the auxin system and/or on the dynamics of downstream responses.…”

Section: Discussionmentioning

confidence: 99%

A Synthetic Approach Allows Rapid Characterization of the Maize Nuclear Auxin Response Circuit

et al. 2020

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Auxin plays a key role across all land plants in growth and developmental processes. Although auxin signaling function has diverged and expanded, differences in the molecular functions of signaling components have largely been characterized in Arabidopsis (Arabidopsis thaliana). Here, we used the nuclear Auxin Response Circuit recapitulated in yeast (Saccharomyces cerevisiae) system to functionally annotate maize (Zea mays) auxin signaling components, focusing on genes expressed during the development of ear and tassel inflorescences. All 16 maize auxin/indole-3-acetic acid repressor proteins were degraded in response to auxin with rates that depended on both receptor and repressor identities. When fused to the maize TOPLESS homolog RAMOSA1 ENHANCER LOCUS2, maize auxin/indole-3-acetic acids were able to repress AUXIN RESPONSE FACTOR transcriptional activity. A complete auxin response circuit comprising all maize components, including the ZmAFB2/3 b1 maize AUXIN SIGNALING F-BOX (AFB) receptor, was fully functional. The ZmAFB2/3 b1 auxin receptor was more sensitive to hormone than AtAFB2 and allowed for rapid circuit activation upon auxin addition. These results validate the conserved role of predicted auxin response genes in maize as well as provide evidence that a synthetic approach can facilitate broader comparative studies across the wide range of species with sequenced genomes.

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Section: Discussionmentioning

confidence: 99%

A Synthetic Approach Allows Rapid Characterization of the Maize Nuclear Auxin Response Circuit

et al. 2020

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“…Recent developments in fluorescent marker design in both maize (Wu et al, 2013;Krishnakumar et al, 2015) and rice (Yang et al, 2017), specifically the development of fluorescent auxin biosensors (Mir et al, 2017;Yang et al, 2017), have greatly improved molecular and functional characterization approaches of auxin genes in monocots. Functional studies will further profit from newly developed biochemical inhibitors that, in addition to the currently available auxin transport inhibitors, now enable various aspects of auxin biosynthesis and signaling to be inhibited (Hasegawa et al, 2018;Ma et al, 2018).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Auxin EvoDevo: Conservation and Diversification of Genes Regulating Auxin Biosynthesis, Transport, and Signaling

et al. 2019

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The phytohormone auxin has been shown to be of pivotal importance in growth and development of land plants. The underlying molecular players involved in auxin biosynthesis, transport, and signaling are quite well understood in Arabidopsis. However, functional characterizations of auxin-related genes in economically important crops, specifically maize and rice, are still limited. In this article, we comprehensively review recent functional studies on auxin-related genes in both maize and rice, compared with what is known in Arabidopsis, and highlight conservation and diversification of their functions. Our analysis is illustrated by phylogenetic analysis and publicly available gene expression data for each gene family, which will aid in the identification of auxin-related genes for future research. Current challenges and future directions for auxin research in maize and rice are discussed. Developments in gene editing techniques provide powerful tools for overcoming the issue of redundancy in these gene families and will undoubtedly advance auxin research in crops.

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“…S4), suggested that, in ndl1 IMs, the levels of free auxin may be decreased and thus cause early defects in organogenesis. To test this hypothesis, we crossed ndl1 to DII-VENUS, a marker line based on the degron domain of AUX/IAA proteins, whose degradation is controlled by auxin levels in cells (37,38). In wild type tassels, low expression of DII-VENUS was detected in the IM, indicating relatively high concentration of endogenous auxin.…”

Section: Significancementioning

confidence: 99%

NEEDLE1 encodes a mitochondria localized ATP-dependent metalloprotease required for thermotolerant maize growth

Liu

Galli

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Meristems are highly regulated structures ultimately responsible for the formation of branches, lateral organs, and stems, and thus directly affect plant architecture and crop yield. In meristems, genetic networks, hormones, and signaling molecules are tightly integrated to establish robust systems that can adapt growth to continuous inputs from the environment. Here we characterized needle1 (ndl1), a temperature-sensitive maize mutant that displays severe reproductive defects and strong genetic interactions with known mutants affected in the regulation of the plant hormone auxin. NDL1 encodes a mitochondria-localized ATP-dependent metalloprotease belonging to the FILAMENTATION TEMPERATURE-SENSITIVE H (FTSH) family. Together with the hyperaccumulation of reactive oxygen species (ROS), ndl1 inflorescences show up-regulation of a plethora of stress-response genes. We provide evidence that these conditions alter endogenous auxin levels and disrupt primordia initiation in meristems. These findings connect meristem redox status and auxin in the control of maize growth.

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A DII Domain-Based Auxin Reporter Uncovers Low Auxin Signaling during Telophase and Early G1

Cited by 14 publications

References 48 publications

A Synthetic Approach Allows Rapid Characterization of the Maize Nuclear Auxin Response Circuit

A Synthetic Approach Allows Rapid Characterization of the Maize Nuclear Auxin Response Circuit

Auxin EvoDevo: Conservation and Diversification of Genes Regulating Auxin Biosynthesis, Transport, and Signaling

NEEDLE1 encodes a mitochondria localized ATP-dependent metalloprotease required for thermotolerant maize growth

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