A dialysis-based in vitro drug release assay to study dynamics of the drug-protein transfer of temoporfin liposomes

Wallenwein, Chantal M.; Nova, Mônica Villa; Janas, Christine; Jablonka, Laura; Gao, Ge; Thurn, Manuela; Albrecht, Volker; Wiehe, Arno; Wacker, Matthias G.

doi:10.1016/j.ejpb.2019.08.010

Cited by 33 publications

(28 citation statements)

References 29 publications

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“…Unfortunately, several of the techniques used for this purpose, such as filtration and centrifugation, may degrade the carriers or promote drug release [154]. Dialysis-based methods present the advantage of being much softer and allow online detection, although they are not suitable for rapidly released drugs, as membrane transfer itself may become rate-limiting [155].…”

Section: Evaluation Of Drug Release From Polymeric Nanocarriersmentioning

confidence: 99%

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

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The importance of polymeric nanocarriers in the field of drug delivery is ever-increasing, and the accurate characterization of their properties is paramount to understand and predict their behavior. Asymmetric flow field-flow fractionation (AF4) is a fractionation technique that has gained considerable attention for its gentle separation conditions, broad working range, and versatility. AF4 can be hyphenated to a plurality of concentration and size detectors, thus permitting the analysis of the multifunctionality of nanomaterials. Despite this potential, the practical information that can be retrieved by AF4 and its possible applications are still rather unfamiliar to the pharmaceutical scientist. This review was conceived as a primer that clearly states the “do’s and don’ts” about AF4 applied to the characterization of polymeric nanocarriers. Aside from size characterization, AF4 can be beneficial during formulation optimization, for drug loading and drug release determination and for the study of interactions among biomaterials. It will focus mainly on the advances made in the last 5 years, as well as indicating the problematics on the consensus, which have not been reached yet. Methodological recommendations for several case studies will be also included. Graphical abstract

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Section: Evaluation Of Drug Release From Polymeric Nanocarriersmentioning

confidence: 99%

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Quattrini

Berrecoso

Crecente‐Campo

et al. 2021

Drug Deliv. and Transl. Res.

View full text Add to dashboard Cite

show abstract

“…The DM method is a widely used and versatile method for testing in vitro drug release of particulate formulations including microspheres [ 42 ], nanoparticles [ 43 ], and liposomes [ 44 , 45 ]. This method utilizes an appropriate dialysis membrane with a specific molecular-weight cut-off (MWCO) to physically separate the released drug molecules from microparticles by allowing the drug to pass easily through the membrane into the release medium.…”

Section: In Vitro Drug Release Testing Methodsmentioning

confidence: 99%

“…Therefore, the DM method minimizes the possibility of particle loss during sampling process. Disadvantage of this method is that due to the inherent barrier properties of the dialysis membrane, the equilibrium between the inside and outside of the dialysis bag is slowed, which may limit the accurate analysis of initial drug levels in formulations with high burst release [ 45 ]. Additionally, this method cannot be used if the drug is bound to particles or dialysis membrane.…”

Section: In Vitro Drug Release Testing Methodsmentioning

confidence: 99%

Recent Progress in Drug Release Testing Methods of Biopolymeric Particulate System

et al. 2021

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Biopolymeric microparticles have been widely used for long-term release formulations of short half-life chemicals or synthetic peptides. Characterization of the drug release from microparticles is important to ensure product quality and desired pharmacological effect. However, there is no official method for long-term release parenteral dosage forms. Much work has been done to develop methods for in vitro drug release testing, generally grouped into three major categories: sample and separate, dialysis membrane, and continuous flow (flow-through cell) methods. In vitro drug release testing also plays an important role in providing insight into the in vivo performance of a product. In vitro release test with in vivo relevance can reduce the cost of conducting in vivo studies and accelerate drug product development. Therefore, investigation of the in vitro–in vivo correlation (IVIVC) is increasingly becoming an essential part of particulate formulation development. This review summarizes the principles of the in vitro release testing methods of biopolymeric particulate system with the recent research articles and discusses their characteristics including IVIVC, accelerated release testing methods, and stability of encapsulated drugs.

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“…The dialysis membrane method determined the leakage rate of liposome (Wallenwein et al., 2019 ). Concisely, the liposomes were dialyzed in PBS buffer at 4 °C and 25 °C with continuous stirring for 14 days.…”

Section: Methodsmentioning

confidence: 99%

DEC-205 receptor-mediated long-circling nanoliposome as an antigen and Eucommia ulmoides polysaccharide delivery system enhances the immune response via facilitating dendritic cells maturation

et al. 2020

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A dialysis-based in vitro drug release assay to study dynamics of the drug-protein transfer of temoporfin liposomes

Cited by 33 publications

References 29 publications

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Asymmetric flow field-flow fractionation as a multifunctional technique for the characterization of polymeric nanocarriers

Recent Progress in Drug Release Testing Methods of Biopolymeric Particulate System

DEC-205 receptor-mediated long-circling nanoliposome as an antigen and Eucommia ulmoides polysaccharide delivery system enhances the immune response via facilitating dendritic cells maturation

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