A detrimental effect of platelets on mouse embryo development

Jinno, M.; Iida, E; Iizuka, R

doi:10.1007/bf01555379

Cited by 6 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is based on observations that blastocyst outgrowth, the in-vitro equivalent to nidation, occurs only in the presence of whole serum and not with serum derived from platelet-depleted plasma (O'Neill, 1985a;O'Neill et ai, 1989). This conclusion is not supported by evidence showing that the products of activated platelets are detrimental to the development of ICR mouse embryos in vitro (Jinno et al, 1987).…”

Section: Discussionmentioning

confidence: 79%

Failure to detect platelet-activating factor using the splenectomized mouse bioassay

Scodras¹,

Betteridge²,

Croy³

et al. 1991

Reproduction

View full text Add to dashboard Cite

The ability of purified preparations of platelet-activating factor (PAF), from three different suppliers, to induce thrombocytopaenia in mice after splenectomy and to activate mouse platelets in vitro was examined. Although the PAF preparations were potent activators of horse and cow platelets in vitro, injections of up to 1 microgram PAF failed to elicit thrombocytopaenia responses in either CD1 or Swiss Webster random-bred mice. However, when thrombin was injected into Swiss Webster mice, a dose-dependent decrease in the concentration of platelets was observed. Furthermore, isolated platelets from these strains and from 3 inbred lines (C3H/He, BALB/c, C57BL/6) of mice, were not aggregated by PAF in vitro but were sensitive to adenosine diphosphate and thrombin. No change in circulating platelet concentrations was observed over the initial 7 days of gestation in intact Swiss Webster and C57BL/6 or splenectomized C57BL/6 mice, suggesting either an absence of PAF production during early pregnancy in these strains or insensitivity of their platelets to PAF. These results suggest that many mouse strains are unsuitable for the bioassay of PAF.

show abstract

Section: Discussionmentioning

confidence: 79%

Failure to detect platelet-activating factor using the splenectomized mouse bioassay

Scodras¹,

Betteridge²,

Croy³

et al. 1991

Reproduction

View full text Add to dashboard Cite

show abstract

“…(Dandekar andQuigley, 1984, McDowell et al, 1988). The end point of the MEB is generally the development of mouse embryos to the blastocyst stage, the number of hatching/hatched blastocysts (Dandekar and Quigley, 1984;Gianaroli et al, 1986;Schneider, 1986;Jinno et al, 1987;Ogawa and Marrs, 1987;Ogawa et al, 1987;Ball and Aaker, 1989) blastomere cell numbers (Scott et al, 1993;Gardner et al, 1997), number of developing fetuses (Caro and Trounson, 1984;Arny et al, 1987;Han and Kiessling, 1988) or live pups. (Mahadevan et al, 1986;Whitten and Biggers, 1968).…”

Section: Toxicity Testingmentioning

confidence: 99%

Quality Management Issues in the Assisted Reproduction Laboratory

Boone

Higdon

Johnson

2010

Journal of Reproductive and Stem Cell Biotechnology

View full text Add to dashboard Cite

In the United States, the Clinical Laboratory Improvement Act (CLIA) of 1988 describes requirements and guidelines for implementing a quality control/quality assurance (QC/QA) program for moderate and high complexity laboratories. These requirements and guidelines apply to Assisted Reproductive Technology (ART) laboratories as well. The general topic of QC and QA as it pertains to in vitro fertilization (IVF) and embryo transfer (ET) is extensively reviewed. This review summarizes many of the QC and QA events that contribute to the advancement of knowledge in this biotechnological field. These events include control of the culture environment inside and outside of the incubator, as well as factors that affect culture media. This review also discusses, in considerable detail, the QC and the QA that pertain to equipment used within the laboratory and how to control for potential contaminants, which reside within the laboratory. This review provides evidence to indicate the need for laboratory personnel to monitor quality improvement issues on a continuous basis. Personnel must be willing to change as improvements in technology occur in order to meet the ever-evolving demands of a more difficult patient population. Suggestions for meeting these demands are offered.

show abstract

Optimization and simplification of culture conditions in human in vitro fertilization (IVF) and preembryo replacement by serum-free media

Holst

Bertheussen

Forsdahl

et al. 1990

J Assist Reprod Genet

View full text Add to dashboard Cite

The results of 220 consecutive IVF treatments are presented, comparing the use of culture media supplemented with either patient serum (Group 1; n = 110), or Medi-Cult SSR 2 synthetic serum replacement with pyruvate, and human serum albumin (HSA) (GEA BioTech, Hvidovre, Denmark) (Group 2; n = 110). In both groups the Medi-Cult Hybritest was used for routine quality testing. A significantly (P less than 0.05) increased rate of deliveries/ongoing pregnancies was observed with the Group 2 medium. However, no significant differences in fertilization rate, cleavage rate, or implantation rate were observed. It is concluded that the serum-free culture medium described and the testing for absence of cytotoxicity in a sensitive bioassay (Hybritest) have yielded culture conditions capable of sustaining the development in vitro of human preembryos without impairing the fertilization process or the implantation rate, ultimately resulting in a significantly increased rate of deliveries/ongoing pregnancies and an apparently decreased abortion rate. The potential harmful effects of serum and the need for blood sampling and preparation further increase the advantages of replacing serum with the synthetic serum replacement SSR 2 in an IVF program.

show abstract

A detrimental effect of platelets on mouse embryo development

Cited by 6 publications

References 9 publications

Failure to detect platelet-activating factor using the splenectomized mouse bioassay

Failure to detect platelet-activating factor using the splenectomized mouse bioassay

Quality Management Issues in the Assisted Reproduction Laboratory

Optimization and simplification of culture conditions in human in vitro fertilization (IVF) and preembryo replacement by serum-free media

Contact Info

Product

Resources

About