Optimization and simplification of culture conditions in human in vitro fertilization (IVF) and preembryo replacement by serum-free media

Holst, N.; Bertheussen, Kjell; Forsdahl, F; Håkonsen, Mona Berger; Hansen, Lars Jul; Nielsen, Hans Ingolf

doi:10.1007/bf01133884

Cited by 35 publications

(26 citation statements)

References 17 publications

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“…Conflicting results in the literature about sex ratio after long term culture gave the stimulus to analyse the results from our clinic, which has several years of experience (since 1994) with long term culture in human IVF, always using sequential culture media [6,7,14,15]. We performed embryo transfer between day 2 and day 5.…”

Section: Discussionmentioning

confidence: 99%

Blastocyst transfer and gender: IVF versus ICSI

Hentemann

Briskemyr

Bertheussen

2009

J Assist Reprod Genet

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Purpose To investigate the difference in sex ratio in our centre after day 5 embryo transfer among neonates delivered after fresh in vitro fertilization (IVF), fresh intra cytoplasmatic sperminjection (ICSI) and frozen embryo replacement (FER) compared to expected sex ratio in normal population. Methods Retrospective data after IVF/ICSI/FER therapy from 1995 -2007 and data from the literature on similar subjects. Results Among a total of 420 births after day 5 transfer, there were 225 male and 195 female babies. However, after ICSI alone 30 were female and 21 male. Adding all available data from the literature on gender ratio after long term culture, the ratio of male births to total births (0.545) was significantly (p=0.004) different from the expected ratio (0.515) Conclusions It appears that significantly more males are born than expected after day 5 transfer in IVF. However, a subgroup analysis in our data comparing IVF, ICSI and FER revealed more females born after ICSI and FER, suggesting that further research is needed in this area.

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Section: Discussionmentioning

confidence: 99%

Blastocyst transfer and gender: IVF versus ICSI

Hentemann

Briskemyr

Bertheussen

2009

J Assist Reprod Genet

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“…Despite the very early recognition and adaptation of this concept in clinical work (Holst et al, 1990), only recently has wider application been allowed in emerging areas of stem cell research (Amit et al, 2004;Li et al, 2005), and MSC research has not been unscatched by this principle (Tao et al, 2005).…”

Section: Us Food and Drug Administrationmentioning

confidence: 99%

From bone marrow to therapeutic applications: different behaviour and genetic/epigenetic stability during mesenchymal stem cell expansion in autologous and foetal bovine sera?

Tonti¹,

Mannello²

2008

Int. J. Dev. Biol.

107

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Bone marrow-derived mesenchymal stem cells are a multipotent adult cellular population endowed with broad differentiation potential. Their regeneration capability, ease to undergo gene modifications, and immuno-suppressive capacity makes them optimal tools for tissue engineering, gene-and immuno-therapy. Due to the ever-increasing number of studies on the clinical applications of mesenchymal stem cells in regenerative medicine, these cells have become attractive targets in clinical transplantation. However, the identification and definition of mesenchymal stem cell culture media for their clinical application in cell therapy is currently a matter of strong discussion. Up to now, clinical studies have been conducted with mesenchymal stem cells cultured in foetal calf serum, and the chance of contamination or immunological reaction towards xenogeneic compounds must be taken into consideration. On the other hand, a serum-free medium without the addition of growth factors is not able to expand these cells in vitro; so the evaluation of which is best, among foetal calf serum, human serum (whether autologous or allogeneic) and platelet-rich plasma, is a "hot topic" urgently needing further research efforts. The need for the establishment of standardized protocols for mesenchymal stem cell preparations, in order not to interfere with their self-renewal and differentiation processes, assuring durable engraftment and long-term therapeutic effects, is evidently crucial. Therefore, the search for optimal culture conditions for the effective clinical-scale production of vast numbers of mesenchymal stem cells for cellular therapy is of paramount importance and the need for a robust passage from basic to translational research is fundamental. KEY WORDS: AB serum, autologous serum, mesenchymal stem cell, platelet-rich medium The starting pointThe first isolation of fibroblast-like colonies from bone marrow falls back to 1970, when Friedenstein reported the isolation of marrow-derived fibroblast-like cells, able to adhere to the plastic substrate of the cell culture plate (Friedenstein et al., 1970). On this basis, many approaches have been investigated and several protocols have been established to prepare primary cultures of bone marrow-derived mesenchymal stem cells (MSC) with more homogeneous cell population (Pittenger et al., 1999;Jiang et al., 2002). These MSCs are defined as self-renewable, multipotent progenitor cells with the capacity to differentiate into lineagespecific cells that form bone, cartilage, fat, tendon and muscle Int. J. Dev. Biol. 52: 1023-1032 (2008) tissue; in addition to the capacity to differentiate into their natural derivatives, MSCs have the capability to differentiate into other types of cells, giving rise to hepatic, renal, cardiac and neural cells (Song and Tuan, 2004;Pansky et al., 2007). Moreover, MSCs have been isolated not only from bone marrow, but also from other tissues such as adipose tissue, skeletal muscle, trabecular bone, liver, brain, placenta, deciduous teeth, pancrea...

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“…The women received 150 IU human menopausal gonadotropin (hMG, Organon, Oss, The Netherlands) from day three of the menstrual cycle until appropriate response, evaluated by daily serum oestradiol measurements and ultrasound examinations. Ovulation was induced by an injection of 9000IU of human chorionic gonadotropin (hCG) (Physex, Leo, Copenhagen, Denmark), and oocyte aspiration performed approximately 35 h later [12].…”

Section: Methodsmentioning

confidence: 99%

Effects of ovarian stimulation on blood pressure and plasma catecholamine levels

Tollan

Øian

Kjeldsen

et al. 1993

Scandinavian Journal of Clinical and Laboratory Investigation

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Effects of ovarian stimulation for in vitro fertilization on blood pressure and plasma catecholamine levels were studied in 10 women. The examinations were carried out before hormonal treatment with human menopausal gonadotropin (day three of the menstrual cycle, mean serum oestradiol concentration 0.2 nmol l-1, and on the day after ovulation induction with human chorionic gonadotropin (cycle days 10-12, mean serum oestradiol concentration 7.4 nmol l-1). Systolic and diastolic blood pressures (mean +/- SD) decreased 6.7 +/- 8.6 mm Hg, p = 0.049, and 5.3 +/- 4.7 mm Hg, p = 0.009, respectively), and venous plasma noradrenaline increased (42 +/- 44 pg ml-1, p = 0.02) during ovarian stimulation. No significant change was observed in either arterial noradrenaline, arterial adrenaline or venous adrenaline. After stimulation a positive correlation was observed between systolic blood pressure and arterial adrenaline (r = 0.73, p = 0.027), and between systolic blood pressure and the arterial-venous difference for adrenaline (r = 0.81, p = 0.007). The increased venous noradrenaline levels may be a reflex-mediated activation of the sympathetic nervous tone due to a decrease in blood pressure, or may indicate reduced neuronal re-uptake of released noradrenaline. The mechanisms behind the strong correlation between adrenaline and blood pressure are unclear, but may be induced by the supraphysiological oestradiol levels. Thus, adrenaline seems to be more important for blood pressure control in this particular setting.

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Optimization and simplification of culture conditions in human in vitro fertilization (IVF) and preembryo replacement by serum-free media

Cited by 35 publications

References 17 publications

Blastocyst transfer and gender: IVF versus ICSI

Blastocyst transfer and gender: IVF versus ICSI

From bone marrow to therapeutic applications: different behaviour and genetic/epigenetic stability during mesenchymal stem cell expansion in autologous and foetal bovine sera?

Effects of ovarian stimulation on blood pressure and plasma catecholamine levels

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