A Detoxification-Free Process for Enhanced Ethanol Production From Corn Fiber Under Semi-Simultaneous Saccharification and Fermentation

Guo, Yingjie; Huang, Jiamin; Xu, Nuo; Jia, Hexue; Li, Xuezhi; Zhao, Jian; Qu, Yinbo

doi:10.3389/fmicb.2022.861918

Cited by 3 publications

(3 citation statements)

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“…The optimized hydrolysate presents a complex composition, containing not only glucose and xylose but also certain inhibitors, this is a drawback associated with dilute acid pretreatment [ 47 ]. According to the literature reports, if the concentration of acetic acid exceeds 5.0 g/L or that of furfural surpasses 0.2 g/L, it may exert adverse effects on the growth and fermentation of S cerevisiae [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…The optimized hydrolysate presents a complex composition, containing not only glucose and xylose but also certain inhibitors, this is a drawback associated with dilute acid pretreatment [47]. According to the literature reports, if the concentration of acetic acid The more red the color, the higher the sugar yield, the more blue the color, the lower the sugar yield.…”

Section: Evaluation Of Yeast Fermentation Of Poplar Biomass Hydrolysatesmentioning

confidence: 99%

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Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Xu,

Sun,

Wang

et al. 2024

Microorganisms

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Cellulosic ethanol is the key technology to alleviate the pressure of energy supply and climate change. However, the ethanol production process, which is close to industrial production and has a high saccharification rate and ethanol yield, still needs to be developed. This study demonstrates the effective conversion of poplar wood waste into fuel-grade ethanol. By employing a two-step pretreatment using sodium chlorite (SC)-dilute sulfuric acid (DSA), the raw material achieved a sugar conversion rate exceeding 85% of the theoretical value. Under optimized conditions, brewing yeast co-utilizing C6/C5 enabled a yield of 35 g/L ethanol from 10% solid loading delignified poplar hydrolysate. We increased the solid loading to enhance the final ethanol concentration and optimized both the hydrolysis and fermentation stages. With 20% solid loading delignified poplar hydrolysate, the final ethanol concentration reached 60 g/L, a 71.4% increase from the 10% solid loading. Our work incorporates the pretreatment, enzymatic hydrolysis, and fermentation stages to establish a simple, crude poplar waste fuel ethanol process, expanding the range of feedstocks for second-generation fuel ethanol production.

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Section: Resultsmentioning

confidence: 99%

“…The optimized hydrolysate presents a complex composition, containing not only glucose and xylose but also certain inhibitors, this is a drawback associated with dilute acid pretreatment [47]. According to the literature reports, if the concentration of acetic acid The more red the color, the higher the sugar yield, the more blue the color, the lower the sugar yield.…”

Section: Evaluation Of Yeast Fermentation Of Poplar Biomass Hydrolysatesmentioning

confidence: 99%

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Xu,

Sun,

Wang

et al. 2024

Microorganisms

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“…Different from this, Zhang et al ( 2021 ) adopted biodetoxification by inoculating Paecilomyces variotii to remove inhibitors produced by citric acid pretreatment, and the ethanol concentration reached 70.2 g/L using semi-SSF at 25% of solid content, and ethanol production reached 0.280 g/g initial corn fiber. Guo et al ( 2022 ) optimized the pretreatment conditions by response surface methodology to obtain high yield fermentable sugars and reduce the formation of inhibitors, the mixture of solid and hydrolysates obtained by dilute acid pretreatment of corn fiber was directly used for semi-simultaneous saccharification and fermentation without detoxification, and the ethanol yield reached about 81% of the theoretical yield.…”

Section: Status and Progress Of Cellulosic Ethanol Production Technol...mentioning

confidence: 99%

Production of cellulosic ethanol and value-added products from corn fiber

Guo

Liu

Ning

et al. 2022

Bioresour. Bioprocess.

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Corn fiber, a by-product from the corn processing industry, mainly composed of residual starch, cellulose, and hemicelluloses, is a promising raw material for producing cellulosic ethanol and value-added products due to its abundant reserves and low costs of collection and transportation. Now, several technologies for the production of cellulosic ethanol from corn fiber have been reported, such as the D3MAX process, Cellerate™ process, etc., and part of the technologies have also been used in industrial production in the United States. The ethanol yields range from 64 to 91% of the theoretical maximum, depending on different production processes. Because of the multicomponent of corn fiber and the complex structures highly substituted by a variety of side chains in hemicelluloses of corn fiber, however, there are many challenges in cellulosic ethanol production from corn fiber, such as the low conversion of hemicelluloses to fermentable sugars in enzymatic hydrolysis, high production of inhibitors during pretreatment, etc. Some technologies, including an effective pretreatment process for minimizing inhibitors production and maximizing fermentable sugars recovery, production of enzyme preparations with suitable protein compositions, and the engineering of microorganisms capable of fermenting hexose and pentose in hydrolysates and inhibitors tolerance, etc., need to be further developed. The process integration of cellulosic ethanol and value-added products also needs to be developed to improve the economic benefits of the whole process. This review summarizes the status and progresses of cellulosic ethanol production and potential value-added products from corn fiber and presents some challenges in this field at present.

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High-Efficient Production of Cellulosic Ethanol from Corn Fiber Based on the Suitable C5/C6 Co-Fermentation Saccharomyces cerevisiae Strain

Zhao

et al. 2023

Fermentation

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As a potential alternative to fossil-based fuels, cellulosic ethanol has attracted much attention due to its great benefit to energy sustainability and environmental friendliness. However, at present, the industrial competitiveness of cellulosic ethanol production is still insufficient compared with fossil-based fuels because of the higher costs. Expanding the range of lignocellulosic biomass may be a promising measure to promote the economical production of cellulosic ethanol. Corn fiber, a byproduct from the corn deep-processing, is an attractive feedstock for cellulosic ethanol production because of its rich carbohydrate content (generally exceeding 65% of dry weight), almost no transportation cost, and low lignin content allow it to be easily handled. This study first optimized the hydrolysis conditions, including the pretreatment and enzymolysis process based on dilute sulfuric acid, to achieve a high sugar yield. Then, the corn fiber hydrolysates obtained under different hydrolysis conditions were suitably fermented by different C5/C6 co-fermentation Saccharomyces cerevisiae, indicating that the hydrolysate at high solid loading (20%) needs to detoxification to a certain extent but not low solid loading (10%) to achieve high ethanol yield. Finally, the fermentation of the 20% solid loading hydrolysates with resin detoxification was performed in a 50 L bioreactor, achieving the sugar (glucose and xylose) metabolic rate of 2.24 g L −1 h −1 and ethanol yield of 92% of the theoretical value, which are the highest reported levels to date. This study provided a potential process route for cellulosic ethanol production from corn fiber from the perspective of the suitability between the upstream hydrolysis process and the downstream fermentation strain.

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A Detoxification-Free Process for Enhanced Ethanol Production From Corn Fiber Under Semi-Simultaneous Saccharification and Fermentation

Cited by 3 publications

References 46 publications

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Highly Efficient Production of Cellulosic Ethanol from Poplar Using an Optimal C6/C5 Co-Fermentation Strain of Saccharomyces cerevisiae

Production of cellulosic ethanol and value-added products from corn fiber

High-Efficient Production of Cellulosic Ethanol from Corn Fiber Based on the Suitable C5/C6 Co-Fermentation Saccharomyces cerevisiae Strain

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