A desktop multi-material 3D bio-printing system with open-source hardware and software

Lee, Jaehoo; Kim, Kyu Eon; Bang, Sumi; Noh, Insup; Lee, Chibum

doi:10.1007/s12541-017-0072-x

Cited by 49 publications

(29 citation statements)

References 25 publications

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“…Unlike most of the opensource bioprinters published [54,55], a multi-material 3D bioprinter was also built by installing four units of the printhead without additional modifications ( Figure 3D). This required essentially to print another carriage and some basic components, while other open-source multimaterial 3D bioprinter, like the one designed by Lee et al [56] are far more expensive and complex. We believe our device and the information provided are enough to make users with little expertise in electronics and mechanical systems capable of exchanging a FDM extruder with the new MEBB printhead and create their own 3D bioprinter.…”

Section: Discussionmentioning

confidence: 99%

A Versatile Open-source Printhead for Low-cost 3D Microextrusion-based Bioprinting

Sanz-García¹,

Sodupe-Ortega²,

Pernía‐Espinoza³

et al. 2020

Preprint

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Three-dimensional (3D) bioprinting promises to be essential in tissue engineering for solving the rising demand for organs and tissues. Some bioprinters are commercially available, but their impact on the field of TE is still limited due to their cost or difficulty to tune. Herein, we present a low-cost easy-to-build printhead for microextrusion-based bioprinting (MEBB) that can be installed in many desktop 3D printers to transform them into 3D bioprinters. We can extrude bioinks with precise control of print temperature between 2 - 60 ºC. We validated the versatility of the printhead, by assembling it in three low-cost open-source desktop 3D printers. Multiple units of the printhead can also be easily put together in a single printer carriage for building a multi-material 3D bioprinter. Print resolution was evaluated by creating representative calibration models at different temperatures using natural hydrogels such as gelatin and alginate, and synthetic ones like poloxamer. Using one of the three modified low-cost 3D printers, we successfully printed cell-laden lattice constructs with cell viabilities higher than 90% after 24h post printing. Controlling temperature and pressure according to the rheological properties of the bioinks was essential in achieving optimal printability and great cell viability. The cost per unit of our device, which can be used with syringes of different volume, is less expensive than any other commercially available product. These data demonstrate an affordable open-source printhead with the potential to become a reliable alternative to commercial bioprinters for any laboratory.

show abstract

Section: Discussionmentioning

confidence: 99%

A Versatile Open-source Printhead for Low-cost 3D Microextrusion-based Bioprinting

Sanz-García¹,

Sodupe-Ortega²,

Pernía‐Espinoza³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Unlike most of the open-source bioprinters published [ 61 , 62 ], a multi-material 3D bioprinter was also built by installing four units of the printhead without additional modifications ( Figure 3 D). This required essentially to print another carriage and some basic components, while other open-source multimaterial 3D bioprinter, like the one designed by Lee et al [ 63 ] are far more expensive and complex. We believe our device and the information provided are enough to make users with little expertise in electronics and mechanical systems capable of exchanging an FDM extruder with the new MEBB printhead and create their own 3D bioprinter.…”

Section: Discussionmentioning

confidence: 99%

A Versatile Open-Source Printhead for Low-Cost 3D Microextrusion-Based Bioprinting

et al. 2020

View full text Add to dashboard Cite

Three-dimensional (3D) bioprinting promises to be essential in tissue engineering for solving the rising demand for organs and tissues. Some bioprinters are commercially available, but their impact on the field of Tissue engineering (TE) is still limited due to their cost or difficulty to tune. Herein, we present a low-cost easy-to-build printhead for microextrusion-based bioprinting (MEBB) that can be installed in many desktop 3D printers to transform them into 3D bioprinters. We can extrude bioinks with precise control of print temperature between 2–60 °C. We validated the versatility of the printhead, by assembling it in three low-cost open-source desktop 3D printers. Multiple units of the printhead can also be easily put together in a single printer carriage for building a multi-material 3D bioprinter. Print resolution was evaluated by creating representative calibration models at different temperatures using natural hydrogels such as gelatin and alginate, and synthetic ones like poloxamer. Using one of the three modified low-cost 3D printers, we successfully printed cell-laden lattice constructs with cell viabilities higher than 90% after 24-h post printing. Controlling temperature and pressure according to the rheological properties of the bioinks was essential in achieving optimal printability and great cell viability. The cost per unit of our device, which can be used with syringes of different volume, is less expensive than any other commercially available product. These data demonstrate an affordable open-source printhead with the potential to become a reliable alternative to commercial bioprinters for any laboratory.

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“…Recently, novel strategies in both printing technology and materials have been suggested. A sophisticated and multimodal injection system, the systematic development of a 3D printing module, and a sacrificial mold to create a more stable structure have been suggested [36,[66][67][68][69]. The proper selection of a bioink is among the most important factors ensuring successful 3D bioprinting.…”

Section: Three-dimensional Bioprintingmentioning

confidence: 99%

Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

et al. 2020

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Tissue engineering has recently emerged as a novel strategy for the regeneration of damaged skeletal muscle tissues due to its ability to regenerate tissue. However, tissue engineering is challenging due to the need for state-of-the-art interdisciplinary studies involving material science, biochemistry, and mechanical engineering. For this reason, electrospinning and three-dimensional (3D) printing methods have been widely studied because they can insert embedded muscle cells into an extracellular-matrix-mimicking microenvironment, which helps the growth of seeded or laden cells and cell signals by modulating cell–cell interaction and cell–matrix interaction. In this mini review, the recent research trends in scaffold fabrication for skeletal muscle tissue regeneration using advanced techniques, such as electrospinning and 3D bioprinting, are summarized. In conclusion, the further development of skeletal muscle tissue engineering techniques may provide innovative results with clinical potential for skeletal muscle regeneration.

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A desktop multi-material 3D bio-printing system with open-source hardware and software

Cited by 49 publications

References 25 publications

A Versatile Open-source Printhead for Low-cost 3D Microextrusion-based Bioprinting

A Versatile Open-source Printhead for Low-cost 3D Microextrusion-based Bioprinting

A Versatile Open-Source Printhead for Low-Cost 3D Microextrusion-Based Bioprinting

Advanced Techniques for Skeletal Muscle Tissue Engineering and Regeneration

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