A desiccation-related Elip-like gene from the resurrection plant Craterostigma plantagineum is regulated by light and ABA.

Bartels, Dorothea; Hanke, Christian A.; Schneider, Katharina; Michel, Detlef; Salamini, F.

doi:10.1002/j.1460-2075.1992.tb05344.x

Cited by 156 publications

(107 citation statements)

References 49 publications

(39 reference statements)

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“…Dehydration-induced pigment binding ELIP proteins may protect plastids against injury from high-light induced freeradical generation and ultraviolet radiation (Bartels et al 1992) and their induction is a common feature during dehydration of resurrection plants (Gechev et al 2013;Dinakar et al 2012). These proteins are thought to play a role in non-photochemical quenching of light energy and the protection and repair of photosystems during desiccation and appear to be an active participant in the drying induced accumulation of protective pigments in resurrection plants .…”

Section: Desiccation-tolerant Plant Evolution Functional Plant Biologymentioning

confidence: 99%

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

Gaff

Oliver²

2013

Functional Plant Biol.

187

176

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Abstract. In a minute proportion of angiosperm species, rehydrating foliage can revive from airdryness or even from equilibration with air of~0% RH. Such desiccation tolerance is known from vegetative cells of some species of algae and of major groups close to the evolutionary path of the angiosperms. It is also found in the reproductive structures of some algae, moss spores and probably the aerial spores of other terrestrial cryptogamic taxa. The occurrence of desiccation tolerance in the seed plants is overwhelmingly in the aerial reproductive structures; the pollen and seed embryos. Spatially and temporally, pollen and embryos are close ontogenetic derivatives of the angiosperm microspores and megaspores respectively. This suggests that the desiccation tolerance of pollen and embryos derives from the desiccation tolerance of the spores of antecedent taxa and that the basic pollen/embryo mechanism of desiccation tolerance has eventually become expressed also in the vegetative tissue of certain angiosperm species whose drought avoidance is inadequate in microhabitats that suffer extremely xeric episodes. The protective compounds and processes that contribute to desiccation tolerance in angiosperms are found in the modern groups related to the evolutionary path leading to the angiosperms and are also present in the algae and in the cyanobacteria. The mechanism of desiccation tolerance in the angiosperms thus appears to have its origins in algal ancestors and possibly in the endosymbiotic cyanobacteria-related progenitor of chloroplasts and the bacteria-related progenitor of mitochondria. The mechanism may involve the regulation and timing of the accumulation of protective compounds and of other contributing substances and processes.

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Section: Desiccation-tolerant Plant Evolution Functional Plant Biologymentioning

confidence: 99%

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

Gaff

Oliver²

2013

Functional Plant Biol.

187

176

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“…Until recently, few studies dealt with changes in chloroplastic protein synthesis induced by osmotic stress. In Craterostigma plantagineum, a resurrection plant able to tolerate extreme dehydration, three genes preferentially expressed upon desiccation were found to encode chloroplastic desiccation stress proteins, DSP (Bartels et al, 1992;Schneider et al, 1993). Immunological studies revealed that two proteins, DSP 22 and DSP 34, were located in the thylakoids whereas the third DSP 21, weakly homologous to a LEA protein, was found in the stroma.…”

Section: Introductionmentioning

confidence: 99%

A novel thioredoxin‐like protein located in the chloroplast is induced by water deficit in Solanum tuberosum L. plants

et al. 1998

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SummaryBy analysing two-dimensional patterns of chloroplastic proteins from Solanum tuberosum, the authors observed the accumulation of a 32-kDa polypeptide in the stroma of plants subjected to water deficit. N-terminus and internal peptides of the protein, named CDSP 32 for chloroplastic drought-induced stress protein, showed no obvious homology with known sequences. Using a serum raised against the protein N-terminus, a cDNA encoding CDSP 32 was cloned by screening an expression library. The deduced mature CDSP 32 protein is 243 amino acids long and displays typical features of thioredoxins in the C-terminal region (122 residues). In particular, CDSP 32 contains a CGPC motif corresponding to a thioredoxin active site and a number of amino acids conferring thioredoxin-type structure. The CDSP 32 C-terminal region was expressed as a fusion protein in Escherichia coli and was shown to possess thioredoxin activity based on reduction assay of insulin disulfide bridges. RNA blot analysis showed that CDSP 32 transcript does not accumulate upon mild water deficit conditions corresponding to leaf relative water contents (RWC) around 85%, but high levels of CDSP 32 transcripts were observed for more severe stress conditions (RWC around 70%). In vivo labelling and immunoprecipitation revealed a substantial increase in CDSP 32 synthesis upon similar stress conditions. Rewatering of wilted plants caused decreases in both transcript and protein abundances. In tomato wild-type plants and ABAdeficient mutants, a similar accumulation of a CDSP 32-related transcript was observed upon water deficit, most likely indicating no requirement for ABA in the regulation of CDSP 32 synthesis. Based on these results, it is proposed that CDSP 32 plays a role in preservation of the thiol: disulfide redox potential of chloroplastic proteins during water deficit.

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“…In this case, ABA and light were simultaneously required for ELIP-mRNA induction [14]. Since this protein has sequence similarity with ELIP, the effect of ABA on the expression of ELIP was tested under high-light conditions.…”

Section: The Effect Of Abscisic Acid (Aba) On Inducibility Of Elip Mrnamentioning

confidence: 99%

“…Interestingly, the activation by light stress occurs via blue and ultraviolet-A light quality [8, 91. Although closely related proteins have also been found during desiccation stress in Craterostigma [14] and during light stress in the carotenoid-overproducing Dunaliellu [15], the function of ELIP is as yet unknown.Expression of these proteins during development might help to provide additional information to finally understand ELIP induction. The primary leaf of monocotyledonous species is a well-investigated model system in this respect [16] and has already provided some information on ELIP expression during greening [5].…”

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confidence: 99%

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Effects of light stress on the expression of early light‐inducible proteins in barley

Pötter

Kloppstech

1993

European Journal of Biochemistry

100

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Treatment of six-day-old barley leaves with white light of high intensity, 250-2000 W/m2, leads to a linear increase in the steady-state concentrations of early light-inducible protein (ELIP) mRNA followed by an accumulation of the protein. Accumulation of ELIP mRNA, under light stress, is highest in the basal third of the leaf and declines to approximately 50% of this level in the apical segment. The amount of the accumulated protein decreases more steeply towards the tip than would be expected from mRNA levels. This finding, as well as the fact that during greening a massive accumulation of the protein starts only at a time when the steady-state concentrations of ELIP mRNA have declined to 10% of the maximal value, indicate post-transcriptional control. Accumulation is presumably achieved by stabilization of the protein. ELIP mRNA and protein levels, induced by a 2-h period of high-light stress, are lowest in the afternoon and highest at midnight and during the morning. The inducibility of ELIP by high light is therefore under diurnal control. An increase of light stress, due to application of the carotenoid-biosynthesis inhibitor norfluorazon, results in a considerable induction of ELIP mRNA and protein. The plant hormone abscisic acid exerts only a small effect on the mRNA level. In all cases studied, the light-induced increase in the amount of ELIP mRNA was accompanied by a corresponding decline in the mRNA levels for the apoprotein of the chlorophyll-ah-binding protein. Steady-state concentrations of mRNA for the small subunit of ribulose-l,5-bisphosphate carboxylase were hardly affected under all investigated light intensities.The process of thylakoid formation has been thoroughly investigated and although the principle mechanisms are understood the complete details are not yet known [l]. This is especially true for the various mechanisms which precisely regulate photosynthetic efficiency and which have to take place under natural conditions e.g. adaptation to low-light and high-light intensities and also low and high temperatures. During the process of greening, the coordinated expression of genes has been observed [2]. Among the proteins that appear first during greening are early light-inducible proteins (ELIP). These nuclear-encoded thylakoid-membrane proteins share homology with the apoprotein of the chlorophyll-albbinding protein (LHC 11) [3-51 and with the recently discovered 22-kDa protein of photosystem I1 (psbS) [6, 71. The latter similarity seems important as, in contrast to LHC, the binding of pigments has not been detected for ELIP or psbS.During the last two years, ELIP have been recognized as light-stress proteins [8 -101. These studies, derived from earlier findings, showed that after transport into mature chloroplasts ELIP are found in the vicinity of photosystem I1 and can be crosslinked to the D1 protein [ll]. Since D1 is known to rapidly turn over during high-light stress or photoinhibi- tion [12, 131, the expression of ELIP was studied under conditions of light stress. It was found th...

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A desiccation-related Elip-like gene from the resurrection plant Craterostigma plantagineum is regulated by light and ABA.

Cited by 156 publications

References 49 publications

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

The evolution of desiccation tolerance in angiosperm plants: a rare yet common phenomenon

A novel thioredoxin‐like protein located in the chloroplast is induced by water deficit in Solanum tuberosum L. plants

Effects of light stress on the expression of early light‐inducible proteins in barley

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