A description of the type strain of Pseudomonas maltophilia

Hugh, Rudolph; Leifson, Einar

doi:10.1099/0096266x-13-3-133

Cited by 33 publications

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“…The oxidase reaction is positive for every species that we have examined except Pseudomonas maltophilia, which was also reported by Thibault (1961) and by Hugh & Leifson (1963) to be oxidase negative. The biochemical basis of the differences between aerobic bacteria in their response to this commonly used diagnostic test is not known.…”

Section: The Oxidase Reaction and Its Relation To The Cytochrome Systemmentioning

confidence: 49%

“…Strain 72 is aberrant : it can grow a t the expense of D-xylose, isobutyrate, ethanol, n-propanol and L-aspartate, but not on malonate. The type strain of the species, which was proposed by Hugh & Leifson (1963) Pherw typic comparison betweelk Pseudomonas maktophilia and other species of aerobic pseudomonads. The ideal phenotype of P. maltophilia, defined on the basis of a total of ten selected characters, is shown in Table 59.…”

Section: General Propertiesmentioning

confidence: 99%

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The Aerobic Pseudomonads a Taxonomic Study

Stanier

Palleroni

Doudoroff

1966

Journal of General Microbiology

2,660

1,535

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Section: The Oxidase Reaction and Its Relation To The Cytochrome Systemmentioning

confidence: 49%

Section: General Propertiesmentioning

confidence: 99%

The Aerobic Pseudomonads a Taxonomic Study

Stanier

Palleroni

Doudoroff

1966

Journal of General Microbiology

2,660

1,535

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“…Pseudomonas aeruginosa is an example of such an MDRO that causes respiratory infections in patients, particularly tiantibiotic resistance and provides a comparison of clinical and environmental S. maltophilia isolates. (154); later, rRNA cistron analysis determined that it was more appropriately named Xanthomonas maltophilia (259,260,325). In a large study of Xanthomonas strains, an analysis of 295 phenotypic characteristics resulted in 7 strains being identified as X. maltophilia, with 2 of these 7 being type strains of Pseudomonas betle and Pseudomonas hibiscicola (341).…”

mentioning

confidence: 99%

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

2012

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SUMMARY Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen. The increasing incidence of nosocomial and community-acquired S. maltophilia infections is of particular concern for immunocompromised individuals, as this bacterial pathogen is associated with a significant fatality/case ratio. S. maltophilia is an environmental bacterium found in aqueous habitats, including plant rhizospheres, animals, foods, and water sources. Infections of S. maltophilia can occur in a range of organs and tissues; the organism is commonly found in respiratory tract infections. This review summarizes the current literature and presents S. maltophilia as an organism with various molecular mechanisms used for colonization and infection. S. maltophilia can be recovered from polymicrobial infections, most notably from the respiratory tract of cystic fibrosis patients, as a cocolonizer with Pseudomonas aeruginosa . Recent evidence of cell-cell communication between these pathogens has implications for the development of novel pharmacological therapies. Animal models of S. maltophilia infection have provided useful information about the type of host immune response induced by this opportunistic pathogen. Current and emerging treatments for patients infected with S. maltophilia are discussed.

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“…We isolated Stenotrophomonas maltophilia strain WR-C from a clogged septic tank system that consistently formed biofilms on sand grains, produced exopolysaccharides (EPS), and caused clogging in sand columns (33). S. maltophilia is a gram-negative, rod-shaped, and obligate aerobic bacterium with polar flagella in the ␥-␤ subdivision of Proteobacteria (3,14). It is found in various environments and recently has emerged as an important human pathogen.…”

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confidence: 99%

Differential Biofilm Formation and Motility Associated with Lipopolysaccharide/Exopolysaccharide-Coupled Biosynthetic Genes inStenotrophomonas maltophilia

2006

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Stenotrophomonas maltophilia WR-C is capable of forming biofilm on polystyrene and glass. The lipopolysaccharide/exopolysaccharide-coupled biosynthetic genes rmlA, rmlC, and xanB are necessary for biofilm formation and twitching motility. Mutants with mutations in rmlAC and xanB display contrasting biofilm phenotypes on polystyrene and glass and differ in swimming motility.Microorganisms can develop biofilms or clogging mats, causing the failure of septic tanks, systems for on-site wastewater disposal. If water in these clogged systems were contaminated by pathogens, it would pose a threat to human health. We isolated Stenotrophomonas maltophilia strain WR-C from a clogged septic tank system that consistently formed biofilms on sand grains, produced exopolysaccharides (EPS), and caused clogging in sand columns (33). S. maltophilia is a gram-negative, rod-shaped, and obligate aerobic bacterium with polar flagella in the ␥-␤ subdivision of Proteobacteria (3,14). It is found in various environments and recently has emerged as an important human pathogen. Very little is known about the mechanisms of biofilm formation by S. maltophilia. It has been shown to adhere to HEp-2 cells as well as abiotic surfaces, such as plastic, glass, and Teflon (7-10, 16).To identify genes that are involved in biofilm formation by S. maltophilia WR-C, about 4,500 transposon mutants generated with the EZ::TN Ͻ R6K␥ori/KAN-2 Ͼ Tnp transposome (Epicenter, Madison, Wis.) were screened for defects in biofilm formation in 96-well polystyrene plates (Becton-Dickinson Labware, Franklin Lakes, N.J.) by using a modified microtiter plate assay (11,29). Briefly, wells containing 200 l Trypticase soy (TS) broth were inoculated with overnight cultures and incubated at 30°C, 50 rpm for 2 days. Biofilm cells were stained with 0.1% crystal violet and washed, the stain remaining in the cells was solubilized with 70% ethanol, and the optical density at 590 nm was determined. Three mutants, TPH7, TPH11, and TPH13, whose growth was similar to that of the wild type but which were deficient in biofilm formation, were selected for further characterization.The transposon flanking regions were rescued by "rescue cloning" as described by the manufacturer and sequenced by using the primers KAN-2 FP-1 and R6KAN-2 RP-1 (Table 1). The transposon-inserted genes are homologous to three genes involved in the biosynthesis of nucleotide sugar precursors of lipopolysaccharide (LPS) and EPS in Xanthomonas campestris pv. campestris ATCC 33913 (GenBank accession no. AE008922) (6). The genes are rmlC (for TPH7, 74% identity), rmlA (for TPH11, 78% identity), and xanB (for TPH13, 80% identity), which are located in the rml and xan operons of X. campestris pv. campestris (19,20). As no genome sequence is available and the gene cluster involved in LPS/EPS biosynthesis has not been reported for S. maltophilia, the DNA segments flanking the transposon in TPH7, TPH11, and TPH13 were sequenced. The complete sequences of the rmlBACD and xanAB operons and their flanking genes were deter...

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A description of the type strain of Pseudomonas maltophilia

Cited by 33 publications

References 0 publications

The Aerobic Pseudomonads a Taxonomic Study

The Aerobic Pseudomonads a Taxonomic Study

Stenotrophomonas maltophilia: an Emerging Global Opportunistic Pathogen

Differential Biofilm Formation and Motility Associated with Lipopolysaccharide/Exopolysaccharide-Coupled Biosynthetic Genes inStenotrophomonas maltophilia

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