A delta-class glutathione transferase from the Chinese mitten crab Eriocheir sinensis: cDNA cloning, characterization and mRNA expression

De, Zhao; Chen, Liqiao; Qin, Chuanjie; Zhang, Hao; Wu, Ping; Zhang, Fengying

doi:10.1016/j.fsi.2010.06.002

Cited by 42 publications

(25 citation statements)

References 31 publications

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“…4A). This was different with other invertebrate GSTs, whose mRNA expression were significantly up-regulated by gram-negative bacteria challenge [9,19,20], suggesting activation of various GST might on different mechanisms. Compared with LPS, PGN and b-1, 3-glucan could both induce the high expression of SgGST-S1 (Fig.…”

Section: Temporal Expression Of Sggst-s1 Mrna Post Microorganism Glyccontrasting

confidence: 76%

“…After that, it was necessary for hosts to clear the excessive ROS, and several invertebrate GSTs were suspected to be involved in such process, for example, both GST activity and mRNA expression were significantly increased after H. diversicolor was challenged by bacteria [19], while GSTs from E. sinensis and P. martensii transcript levels were also observed to be up-regulated [9,20].…”

Section: Temporal Expression Of Sggst-s1 Mrna Post Microorganism Glycmentioning

confidence: 99%

“…Low concentration of ROS is beneficial for eliminating invaders [6], but the excessive ROS may be detrimental to the hosts' cells [7]. To protect against oxidative stress, aerobic cells employ several antioxidant enzymes to regulate excessive ROS [8,9]. Among of the antioxidant enzymes, glutathione S-transferase (GST) is a superfamily of multifunctional enzymes, which have widely varying catalytic roles [10].…”

Section: Introductionmentioning

confidence: 99%

“…Since the first study in corn 40 years ago [11], increasing GSTs have been identified in other plants and animals [9,12,13]. Based on the similarity of amino acid sequence, over 14 classes cytosolic GSTs have been recognized [10,14].…”

Section: Introductionmentioning

confidence: 99%

“…Several marine GSTs are involved in the immune response against bacterial pathogens [9,19e21]. For instance, the GST activity is activated after Haliotis diversicolor is challenged by bacteria [19], and transcript levels of the GST genes from other marine invertebrates, such as Eriocheir sinensis, Pinctada martensii and Cristaria plicata, can be induced by bacterial pathogen [9,20,21]. Though several GSTs have been identified from aforementioned mollusk, the river of knowledge on molluscan GSTs does not run very deep.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A sigma-class glutathione S-transferase from Solen grandis that responded to microorganism glycan and organic contaminants

Yang

Wei

et al. 2012

Fish & Shellfish Immunology

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Section: Temporal Expression Of Sggst-s1 Mrna Post Microorganism Glyccontrasting

confidence: 76%

Section: Temporal Expression Of Sggst-s1 Mrna Post Microorganism Glycmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A sigma-class glutathione S-transferase from Solen grandis that responded to microorganism glycan and organic contaminants

Yang

Wei

et al. 2012

Fish & Shellfish Immunology

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Genomic and transcriptomic analyses of glutathione S‐transferases in an endoparasitoid wasp, Pteromalus puparum

Teng

et al. 2019

Arch Insect Biochem Physiol

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Pteromalus puparum is a gregarious pupal endoparasitoid with a wide host range. It deposits eggs into pierid and papilionid butterfly pupae. Glutathione S‐transferases (GSTs) are a family of multifunctional detoxification enzymes that act in xenobiotic metabolism in insects. Insect genome projects have facilitated identification and characterization of GST family members. We identified 20 putative GSTs in the P. puparum genome, including 19 cytosolic and one microsomal. Phylogenetic analysis showed that P. puparum GSTs are clustered into Hymenoptera‐specific branches. Transcriptomic data of embryos, larvae, female pupae, male pupae, female adults, male adults, venom glands, carcass, salivary glands, and ovaries revealed stage‐, sex‐, and tissue‐specific expression patterns of GSTs in P. puparum. This is the most comprehensive study of genome‐wide identification, characterization, and expression profiling of GST family in hymenopterans. Our results provide valuable information for understanding the metabolic adaptation of this wasp.

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Identification and expression profile of Delta‐GST in black tiger shrimp (Penaeus monodon) exposed to aflatoxin B1 (AFB1)

et al. 2019

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Delta GST is an insect‐specific class and a prominent class of the glutathione S‐transferases family that is involved in xenobiotic detoxification and antioxidant defense. The full‐length complementary DNA of delta‐class GST from Penaeus monodon (PmDeltaGST; 839 bp long with a 657 bp coding region) was cloned. The encoded polypeptide of 218 amino acids had a predicted molecular mass of 24.30 kDa. Sequence homology and phylogenetic analysis showed that PmDeltaGST was significant similarity to GST genes in crustaceans and insects. Tissue expression profile analysis by quantitative real‐time reverse‐transcription polymerase chain showed that PmDeltaGST was constitutively expressed in all the examined tissues, with the highest expression in hepatopancreas and intestine and the weakest expression in ovary. PmDeltaGST messenger RNA expression and protein levels in hepatopancreas was significantly increased at 14 days postexposure of aflatoxin B1 (AFB1), keeping on the high level at 28 days, but decreased at 56 days. The results suggested that PmDeltaGST was involved in the response to AFB1 exposure.

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A delta-class glutathione transferase from the Chinese mitten crab Eriocheir sinensis: cDNA cloning, characterization and mRNA expression

Cited by 42 publications

References 31 publications

A sigma-class glutathione S-transferase from Solen grandis that responded to microorganism glycan and organic contaminants

A sigma-class glutathione S-transferase from Solen grandis that responded to microorganism glycan and organic contaminants

Genomic and transcriptomic analyses of glutathione S‐transferases in an endoparasitoid wasp, Pteromalus puparum

Identification and expression profile of Delta‐GST in black tiger shrimp (Penaeus monodon) exposed to aflatoxin B1 (AFB1)

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