Genomic and transcriptomic analyses of glutathione S‐transferases in an endoparasitoid wasp, <i>Pteromalus puparum</i>

Xu, Gang; Teng, Ziwen; Gu, Gui-Xiang; Guo, Lei; Wang, Fei; Xiao, Shan; Wang, Jia‐Le; Wang, Beibei; Fang, Qi; Wang, Fang; Song, Qisheng; Stanley, David; Yè, Gōngyín

doi:10.1002/arch.21634

Cited by 4 publications

(3 citation statements)

References 78 publications

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“…Parasitoids frequently encounter various host defenses, including the production of toxic substances as part of host immune responses, thus gene families involved in detoxification and responses to such toxins are crucial for parasitic worms to withstand and overcome these challenges. For instance, studies have highlighted the significance of detoxification enzymes like glutathione S-transferases in facilitating the metabolic adjustments required for the endopar-asitoid wasp Pteromalus puparum to adapt to papilionid butterfly pupae (Xu et al . 2020).…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of parasitoid lifestyle as exemplified by Mermithidae and Nematomorpha

Kirangwa,

Bednarski,

Guiglielmoni

et al. 2024

Preprint

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Mermithidae and Nematomorpha are parasitoids united by the commonalities in their lifestyle – immature stages infect arthropod hosts, species from both phyla can manipulate their host to induce a similar water-seeking behaviour, and both have a final free-living non-feeding adult reproductive stage, often killing their host upon emergence. Some of these species are of great economic importance, being evaluated as biological control agents against mosquito vectors responsible for diseases like malaria, and other insect pests, but with scarce genomic resources currently available. Nematomorpha, despite being closely related to Nematoda, received insufficient attention in genomic research, leading to gaps in our understanding of their diverse genetic makeup. This study aimed to investigate the genetic features encoded in the genomes of both parasitoid taxa to identify similarities and parallels linked to their ecological lifestyles. We performed a comparative analysis of 12 genomes, comprising parasitoid, parasitic and free-living worms. The investigation revealed genomic signatures unique to parasitoid species, including expanded gene families enriched in neural transmission modulation, likely linked to the known host manipulation that both mermithids and nematomorphs exert on their hosts. The analysis also uncovered a diverse array of conserved transposable element superfamilies across both lineages. The findings from this study provide valuable insights into the potential genomic adaptations associated with parasitoidism in nematode and nematomorph worms. The identification of expanded gene families and conserved transposable element superfamilies sheds light on the molecular underpinnings of their unique biological traits. Additionally, the core set of orthologs specific to parasitoid worms offers new avenues for understanding the evolution of parasitism within these groups of organisms.

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Section: Discussionmentioning

confidence: 99%

Comparative genomics of parasitoid lifestyle as exemplified by Mermithidae and Nematomorpha

Kirangwa,

Bednarski,

Guiglielmoni

et al. 2024

Preprint

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“…The annotation of P. puparum related to immune genes, cuticle genes, SPs and their homologs, and GSTs was readily available for DEG analysis [32,33,77,78]. The annotation of other DEGs was obtained from Pfam and Swissprot databases [79,80].…”

Section: Illumina Sequencing and Deg Analysismentioning

confidence: 99%

Altered Gene Expression of the Parasitoid Pteromalus puparum after Entomopathogenic Fungus Beauveria bassiana Infection

Yang,

Li,

Yang

et al. 2023

IJMS

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Both parasitoids and entomopathogenic fungi are becoming increasingly crucial for managing pest populations. Therefore, it is essential to carefully consider the potential impact of entomopathogenic fungi on parasitoids due to their widespread pathogenicity and the possible overlap between these biological control tools during field applications. However, despite their importance, little research has been conducted on the pathogenicity of entomopathogenic fungi on parasitoids. In our study, we aimed to address this knowledge gap by investigating the interaction between the well-known entomopathogenic fungus Beauveria bassiana, and the pupal endoparasitoid Pteromalus puparum. Our results demonstrated that the presence of B. bassiana significantly affected the survival rates of P. puparum under laboratory conditions. The pathogenicity of B. bassiana on P. puparum was dose- and time-dependent, as determined via through surface spraying or oral ingestion. RNA-Seq analysis revealed that the immune system plays a primary and crucial role in defending against B. bassiana. Notably, several upregulated differentially expressed genes (DEGs) involved in the Toll and IMD pathways, which are key components of the insect immune system, and antimicrobial peptides were rapidly induced during both the early and late stages of infection. In contrast, a majority of genes involved in the activation of prophenoloxidase and antioxidant mechanisms were downregulated. Additionally, we identified downregulated DEGs related to cuticle formation, olfactory mechanisms, and detoxification processes. In summary, our study provides valuable insights into the interactions between P. puparum and B. bassiana, shedding light on the changes in gene expression during fungal infection. These findings have significant implications for the development of more effective and sustainable strategies for pest management in agriculture.

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“…1 GST levels are positively associated with expression of highmobility group box-1 (HMGB1) in some diseases. 9,10 HMGB1 acts via the NRF2/GPX4 axis to repress ferroptosis in mesangial cells in response to high glucose. 11 HMGB1 has also been shown to induce ferroptosis via the RAS-JNK/ p38 signaling pathway in leukemia.…”

Section: Introductionmentioning

confidence: 99%

Glutathione S-transferase zeta 1 alters the HMGB1/GPX4 axis to drive ferroptosis in bladder cancer cells

et al. 2023

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Objective The ability of glutathione S-transferase zeta 1 (GSTZ1) to modulate homeostasis of cellular redox and induce ferroptosis was explored in bladder cancer cells, and the involvement of the high mobility group protein 1/glutathione peroxidase 4 (HMGB1/GPX4) in these effects was studied. Methods BIU-87 cells stably overexpressing GSTZ1 were transfected with appropriate plasmids to deplete HMGB1 or overexpress GPX4, then treated with deferoxamine and ferrostatin-1. Antiproliferative effects were assessed by quantifying levels of ferroptosis markers, such as iron, glutathione (GSH), malondialdehyde (MDA), reactive oxygen species (ROS), GPX4, transferrin, and ferritin. Results GSTZ1 was significantly downregulated in bladder cancer cells. GSTZ1 overexpression downregulated GPX4 and GSH, while greatly increasing levels of iron, MDA, ROS, and transferrin. GSTZ1 overexpression also decreased proliferation of BIU-87 cells and activated HMGB1/GPX4 signaling. The effects of GSTZ1 on ferroptosis and proliferation were antagonized by HMGB1 knockdown or GPX4 overexpression. Conclusion GSTZ1 induces ferroptotic cell death and alters cellular redox homeostasis in bladder cancer cells, and these effects involve activation of the HMGB1/GPX4 axis.

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Genomic and transcriptomic analyses of glutathione S‐transferases in an endoparasitoid wasp, Pteromalus puparum

Cited by 4 publications

References 78 publications

Comparative genomics of parasitoid lifestyle as exemplified by Mermithidae and Nematomorpha

Comparative genomics of parasitoid lifestyle as exemplified by Mermithidae and Nematomorpha

Altered Gene Expression of the Parasitoid Pteromalus puparum after Entomopathogenic Fungus Beauveria bassiana Infection

Glutathione S-transferase zeta 1 alters the HMGB1/GPX4 axis to drive ferroptosis in bladder cancer cells

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