A deficiency of interstitial cells of cajal (ICC) in chagasic megacolon

Hagger, R; Finlayson, C.; Kahn, Fredrik; Oliveira, Rsb; Chimelli, Leila; Kumar, Deva Sumanth

doi:10.1016/s0016-5085(98)83104-1

Cited by 6 publications

(6 citation statements)

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“…Neural responses were more resistant to recovery; however, distinct responses were observed in one animal 60 days after removal of the obstruction. Loss of ICC would be expected to result in dramatic motor dysfunction, and numerous studies have documented loss of ICC in GI motor disorders (Faussone‐Pellegrini & Cortesini, 1985; Rumessen, 1996; Vanderwinden et al 1996 a , b ; Isozaki et al 1997; Kenny et al 1998; Hagger et al 1998; He et al 2000; Ordog et al 2000). The fact that ICC losses have been noted in such a variety of GI motor disorders is likely to be very important, however, and it is possible that common molecular signals generated during a variety of pathophysiological processes might converge on factors regulating the ICC phenotype.…”

Section: Discussionmentioning

confidence: 99%

Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction

Chang

Glasgow

Takayama

et al. 2001

The Journal of Physiology

181

192

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1. Partial obstruction of the murine ileum led to changes in the gross morphology and ultrastructure of the tunica muscularis. Populations of interstitial cells of Cajal (ICC) decreased oral, but not aboral, to the site of obstruction. Since ICC generate and propagate electrical slow waves in gastrointestinal muscles, we investigated whether the loss of ICC leads to loss of function in partial bowel obstruction.2. Changes in ICC networks and electrical activity were monitored in the obstructed murine intestine using immunohistochemistry, electron microscopy and intracellular electrophysiological techniques.3. Two weeks following the onset of a partial obstruction, the bowel increased in diameter and hypertrophy of the tunica muscularis was observed oral to the obstruction site. ICC networks were disrupted oral to the obstruction, and this disruption was accompanied by the loss of electrical slow waves and responses to enteric nerve stimulation. These defects were not observed aboral to the obstruction. 6. These data describe the plasticity of ICC networks in response to partial obstruction. After obstruction the ICC phenotype was lost, but these cells regenerated when the obstruction was removed. This model may be an important tool for evaluating the cellular/molecular factors responsible for the regulation and maintenance of the ICC phenotype.

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Section: Discussionmentioning

confidence: 99%

Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction

Chang

Glasgow

Takayama

et al. 2001

The Journal of Physiology

181

192

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“…Kit-ir ICC were reportedly absent, or very rare, in six cases of Chagasic megacolon whereas Kit-ir mast cells were readily identified (Hagger et al, 1998c).…”

Section: Chagasic Megacolonmentioning

confidence: 91%

Interstitial cells of Cajal in human gut and gastrointestinal disease

Vanderwinden

Rumessen

1999

Microsc. Res. Tech.

187

149

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“…Kit labelling has also offered us a greater appreciation for the structure of the anastomosing networks that these cells form with one another and with other cell types throughout the GI tract 32 . Kit immunohistochemistry has recently provided significant information about the loss or disruption of ICC networks in a variety of GI motility disorders 10 including achalasia, 11 diabetes, 12 chronic idiopathic pseudo‐obstruction, 13 Hirschsprung's disease, 16 infantile hypertrophic pyloric stenosis 17 and Chagas disease 18 …”

Section: Discussionmentioning

confidence: 99%

“…Numerous recent studies have utilized Kit labelling to define the loss or changes in these cells that are associated with a variety of GI motility disorders 10 . Loss or disruption in the normal networks of ICC have been reported to occur in patients with achalasia or hypertensive sphincter, 11 diabetes, 12 chronic idiopathic pseudo‐obstruction, 13 partial intestinal obstructions, 14 slow‐transet constipation, 15 Hirschsprung's disease, 16 infantile hypertrophic pyloric stenosis, 17 chagasic megacolon, 18 and GI stromal tumours 19…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical identification of interstitial cells of Cajal in the murine fundus using a live‐labelling technique

Stratton

Ward

Horiguchi

et al. 2006

Neurogastroenterology Motil

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Interstitial cells of Cajal (ICC) within the gastrointestinal (GI) tract play a critical role in the generation of electrical slow waves and as mediators of enteric motor neurotransmission. Kit immunohistochemistry has proven to be a reliable method to identify the location of these cells within the tunica muscularis and to provide information on how the distribution and density of these cells change in a variety of GI motility disorders. Because of the labile nature of Kit or its detection, ultrastructural immunocytochemistry using conventional chemical fixation methods has been difficult. We describe a novel in vivo technique to label ICC within GI tissues. Using antibodies directed against the extracellular domain of the Kit receptor, we have been able to live-label the stomach with Kit while the animal is under anaesthesia and the organ is still receiving normal blood supply. This approach provided optimum maintenance of ultrastructural features with significant binding of antibody to the Kit receptor. The loss of ICC in many human motility disorders suggests exciting new hypotheses for their aetiology. This method will prove useful to investigate the ultrastructural changes that occur in ICC networks in animal models of motility disorders that are associated with the loss of these cells.

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A deficiency of interstitial cells of cajal (ICC) in chagasic megacolon

Cited by 6 publications

References 0 publications

Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction

Loss of interstitial cells of Cajal and development of electrical dysfunction in murine small bowel obstruction

Interstitial cells of Cajal in human gut and gastrointestinal disease

Immunocytochemical identification of interstitial cells of Cajal in the murine fundus using a live‐labelling technique

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