Interstitial cells of Cajal in human gut and gastrointestinal disease

Vanderwinden, Jean‐Marie; Rumessen, Jüri Johannes

doi:10.1002/(sici)1097-0029(19991201)47:5<344::aid-jemt6>3.0.co;2-1

Cited by 187 publications

(162 citation statements)

References 170 publications

(200 reference statements)

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“…The absence of any effects on ICC-MY may be one of the useful merits of DKT to apply to gastrointestinal disorders, since it has been reported that a number of ICC is decreased in many kinds of gastrointestinal disease (Vanderwinden and Rumessen, 1999). DKT seems to improve gastrointestinal symptoms by exerting multiple actions on intestinal motility.…”

Section: Discussionmentioning

confidence: 99%

Effects of Dai-kenchu-to on spontaneous activity in the mouse small intestine

Kito

Suzuki

2006

J. Smooth Muscle Res.

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The effects of Dai-kenchu-to (DKT), a Chinese medicine, on spontaneous activity of mouse small intestine were investigated. Experiments were carried out with tension recording and intracellular recording. DKT contracted mouse longitudinal smooth muscles in a dose dependent manner (0.1-10 mg/ml). Low concentration of DKT (0.1 mg/ml) did not contract the longitudinal muscles of mouse small intestine. DKT (0.1 mg/ml) inhibited contraction elicited by transmural nerve stimulation (TNS). DKT (1 mg/ml) evoked relaxation before contraction. The initial relaxation was abolished by N ω -nitro-L-arginine (L-NNA). DKT (10 mg/ml)-induced contraction had two components: a transient rapid contraction and a following slow contraction. Atropine inhibited DKT (1 mg/ml)-induced contraction to about 50% of control. In the presence of atropine, tetrodotoxin (TTX) inhibited the contraction elicited by DKT (1 mg/ml) to about 80%. DKT depolarized the membrane and decreased the amplitude of pacemaker potentials recorded from in situ myenteric interstitial cells of Cajal (ICC-MY) with no alteration to the frequency, duration and maximum rates of rise in the presence of nifedipine and TTX.The same results were obtained in slow waves recorded from circular smooth muscle cells. These results indicate that DKT evoked both contraction and relaxation by releasing acetylcholine, nitric oxide and other excitatory neurotransmitters in mouse small intestine. DKT had no effects on pacemaker mechanisms and electrical coupling between ICC-MY and smooth muscle cells in mouse small intestine. The results also suggest that DKT may contract smooth muscles by depolarizing the membrane directly.

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Section: Discussionmentioning

confidence: 99%

Effects of Dai-kenchu-to on spontaneous activity in the mouse small intestine

Kito

Suzuki

2006

J. Smooth Muscle Res.

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“…Enteric neurons also innervate smooth muscle and are essential for peristalsis in GI motility [6][7][8][9]. Recently, a large number of studies have reported that loss or defects in ICC and/or enteric neurons could be related to pathophysiology in a variety of motility disorders [8][9][10][11][12]. Thus, there is great interest in understanding the mechanisms that govern the differentiation of ICC and enteric neurons, not only to understand GI motility but also to elucidate motility disorders.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Functional Gut-Like Organ Formation from Mouse Embryonic Stem Cells

et al. 2002

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Background and Aims. Embryonic stem (ES) cellshave a pluripotent ability to differentiate into a variety of cell lineages in vitro. We have recently found that ES cells can give rise to a functional gut-like unit, which forms a three-dimensional dome-like structure with lumen and exhibits mechanical activity, such as spontaneous contraction and peristalsis. The aim of the present study was to investigate the electrophysiological and morphological properties of ES cell-derived contracting clusters.Methods. Electrical activity was examined by an extracellular recording. Morphology and cellular components were investigated by immunohistochemistry and electron microscopy.Results. Clusters with rhythmic contractions displayed electrical slow waves at a regular rhythm, and clusters with highly coordinated peristalsis showed regular slow

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“…In humans, under certain pathophysiological conditions, loss or defects in ICC networks appear to play a role in the generation of certain motility disorders (Vanderwinden et al, 1999;Horisawa et al, 1998). Recent evidence suggests that loss of ICC is associated with numerous gastrointestinal disorders ranging from gastroparesis, pseudoobstruction and idiopathic constipation (Vanderwinden et al, 1999;Sanders et al, 1999;Burns et al, 2007;Rolle et al, 2007;Farrugia et al, 2008). An animal model of ICC loss, the W/WV mouse, has been used extensively to examine functional changes resulting from lesions in ICC (Maeda et al, 1992;Ward et al, 1994;.…”

Section: Pathophysiology Of Icc In Gastrointestinal Tractmentioning

confidence: 99%

“…Indeed, loss of ICCs or disruption of ICC networks has been reported in a wide range of GI diseases, including achalasia, chronic intestinal pseudoobstruction, Hirschsprung disease, inflammatory bowel diseases, slow transit constipation, and others (Vanderwinden et al, 1999;Sanders et al, 2002). Intestinal obstruction may ensue from mechanical or functional causes and, when complete, it may represent a fatal complication of several gastrointestinal disorders.…”

Section: Pathophysiology Of Icc In Other Gastroenteropathymentioning

confidence: 99%

Advances in Research on Pacemaking Function of Interstitial Cell of Cajal in Gastrointestinal Tract

Xu¹

2011

Modern Pacemakers - Present and Future

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Interstitial cells of Cajal in human gut and gastrointestinal disease

Cited by 187 publications

References 170 publications

Effects of Dai-kenchu-to on spontaneous activity in the mouse small intestine

Effects of Dai-kenchu-to on spontaneous activity in the mouse small intestine

In Vitro Functional Gut-Like Organ Formation from Mouse Embryonic Stem Cells

Advances in Research on Pacemaking Function of Interstitial Cell of Cajal in Gastrointestinal Tract

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